Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mntH and nupC are separated by 335 nucleotides nupC and yi81_3 are separated by 86 nucleotides
b2392: mntH - manganese transport protein MntH (NCBI), at 2,509,490 to 2,510,728
mntH
b2393: nupC - nucleoside (except guanosine) transporter (NCBI), at 2,511,064 to 2,512,266
nupC
b2394: yi81_3 - IS186 hypothetical protein (VIMSS), at 2,512,353 to 2,513,465
_3
Position (kb)
2511
2512
2513 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2510.130 kb on + strand, within mntH at 2510.130 kb on + strand, within mntH at 2510.198 kb on + strand, within mntH at 2510.243 kb on + strand, within mntH at 2510.243 kb on + strand, within mntH at 2510.251 kb on - strand, within mntH at 2510.435 kb on - strand, within mntH at 2510.454 kb on - strand, within mntH at 2510.533 kb on - strand, within mntH at 2510.595 kb on - strand, within mntH at 2510.607 kb on - strand at 2510.643 kb on - strand at 2510.708 kb on - strand at 2510.892 kb on + strand at 2510.921 kb on - strand at 2510.921 kb on - strand at 2510.972 kb on + strand at 2510.972 kb on + strand at 2510.974 kb on + strand at 2510.974 kb on + strand at 2510.974 kb on + strand at 2511.001 kb on + strand at 2511.001 kb on + strand at 2511.024 kb on - strand at 2511.061 kb on + strand at 2511.061 kb on + strand at 2511.118 kb on + strand at 2511.169 kb on - strand at 2511.250 kb on - strand, within nupC at 2511.284 kb on + strand, within nupC at 2511.365 kb on + strand, within nupC at 2511.504 kb on + strand, within nupC at 2511.504 kb on + strand, within nupC at 2511.564 kb on + strand, within nupC at 2511.623 kb on - strand, within nupC at 2511.623 kb on - strand, within nupC at 2511.690 kb on + strand, within nupC at 2511.730 kb on - strand, within nupC at 2511.918 kb on + strand, within nupC at 2511.930 kb on - strand, within nupC at 2511.993 kb on - strand, within nupC at 2511.995 kb on + strand, within nupC at 2511.995 kb on + strand, within nupC at 2512.099 kb on - strand, within nupC at 2512.099 kb on - strand, within nupC at 2512.222 kb on - strand at 2512.222 kb on - strand at 2512.227 kb on - strand at 2512.228 kb on + strand at 2512.331 kb on - strand at 2512.331 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 2,510,130 + mntH b2392 0.52 -0.4 2,510,130 + mntH b2392 0.52 -1.0 2,510,198 + mntH b2392 0.57 -3.6 2,510,243 + mntH b2392 0.61 +0.2 2,510,243 + mntH b2392 0.61 -1.7 2,510,251 - mntH b2392 0.61 -1.5 2,510,435 - mntH b2392 0.76 +0.6 2,510,454 - mntH b2392 0.78 -1.2 2,510,533 - mntH b2392 0.84 -1.2 2,510,595 - mntH b2392 0.89 -1.4 2,510,607 - -0.7 2,510,643 - -1.0 2,510,708 - -1.3 2,510,892 + -2.4 2,510,921 - -0.6 2,510,921 - +0.1 2,510,972 + -1.0 2,510,972 + -0.8 2,510,974 + +0.6 2,510,974 + -2.0 2,510,974 + -0.8 2,511,001 + -0.9 2,511,001 + +0.6 2,511,024 - -0.2 2,511,061 + +1.7 2,511,061 + +1.4 2,511,118 + +2.8 2,511,169 - +2.0 2,511,250 - nupC b2393 0.15 +1.5 2,511,284 + nupC b2393 0.18 +1.9 2,511,365 + nupC b2393 0.25 +2.6 2,511,504 + nupC b2393 0.37 +3.2 2,511,504 + nupC b2393 0.37 +2.3 2,511,564 + nupC b2393 0.42 +0.6 2,511,623 - nupC b2393 0.46 +1.7 2,511,623 - nupC b2393 0.46 +2.0 2,511,690 + nupC b2393 0.52 +0.8 2,511,730 - nupC b2393 0.55 +0.3 2,511,918 + nupC b2393 0.71 +2.0 2,511,930 - nupC b2393 0.72 +2.8 2,511,993 - nupC b2393 0.77 +0.8 2,511,995 + nupC b2393 0.77 +2.7 2,511,995 + nupC b2393 0.77 +1.9 2,512,099 - nupC b2393 0.86 +1.9 2,512,099 - nupC b2393 0.86 +1.5 2,512,222 - +0.5 2,512,222 - +2.0 2,512,227 - +1.7 2,512,228 + +1.9 2,512,331 - +1.0 2,512,331 - +0.9
Or see this region's nucleotide sequence