Strain Fitness in Escherichia coli BW25113 around b1294

Experiment: Spectinomycin 0.00625 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Spectinomycin 0.00625 mg/ml
1,352,770	-	sapB	b1293	0.25	-2.3
1,352,770	-	sapB	b1293	0.25	+0.3
1,352,790	+	sapB	b1293	0.27	-0.7
1,353,131	+	sapB	b1293	0.62	-2.0
1,353,522	+				-1.7
1,353,566	-				+1.0
1,353,747	+	sapA	b1294	0.16	+1.0
1,353,747	+	sapA	b1294	0.16	+1.1
1,353,825	+	sapA	b1294	0.20	-0.3
1,353,825	+	sapA	b1294	0.20	-1.0
1,353,841	+	sapA	b1294	0.21	-1.7
1,353,841	+	sapA	b1294	0.21	-2.0
1,353,844	-	sapA	b1294	0.21	+3.2
1,353,890	-	sapA	b1294	0.24	+2.0
1,353,890	-	sapA	b1294	0.24	+1.3
1,353,912	-	sapA	b1294	0.26	+0.8
1,353,987	+	sapA	b1294	0.30	+0.5
1,354,118	-	sapA	b1294	0.38	+0.9
1,354,136	+	sapA	b1294	0.39	-1.0
1,354,136	+	sapA	b1294	0.39	+0.5
1,354,136	+	sapA	b1294	0.39	-0.1
1,354,136	+	sapA	b1294	0.39	-1.6
1,354,320	-	sapA	b1294	0.50	-0.7
1,354,330	-	sapA	b1294	0.51	-0.8
1,354,427	-	sapA	b1294	0.57	+1.6
1,354,486	-	sapA	b1294	0.61	+1.5
1,354,486	-	sapA	b1294	0.61	+2.0
1,354,640	+	sapA	b1294	0.70	-1.5
1,354,789	-	sapA	b1294	0.79	-0.7
1,354,915	-	sapA	b1294	0.87	-0.1
1,354,915	-	sapA	b1294	0.87	+0.5
1,354,998	-				-2.5
1,355,215	+				-1.1
1,355,225	-				+1.3
1,355,225	-				+1.3
1,355,370	-				-0.7
1,355,370	-				+1.3
1,355,445	-				+0.5
1,355,485	+	ymjA	b1295	0.15	-0.3
1,355,488	-	ymjA	b1295	0.17	+0.8
1,355,488	-	ymjA	b1295	0.17	+0.3
1,355,507	-	ymjA	b1295	0.24	-0.7
1,355,713	-				+0.3
1,355,723	-				+0.4
1,355,726	-				+0.7
1,355,728	-				+0.6
1,355,853	-				+0.2
1,355,862	+				-1.6