Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybiS and ybiT are separated by 218 nucleotides ybiT and ybiU are separated by 240 nucleotides
b0819: ybiS - hypothetical protein (NCBI), at 854,047 to 854,967
ybiS
b0820: ybiT - fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 855,186 to 856,778
ybiT
b0821: ybiU - hypothetical protein (NCBI), at 857,019 to 858,284
ybiU
Position (kb) 855
856
857 Strain fitness (log2 ratio) -2
-1
0
1
2 at 854.212 kb on - strand, within ybiS at 854.264 kb on + strand, within ybiS at 854.264 kb on + strand, within ybiS at 854.285 kb on - strand, within ybiS at 854.290 kb on - strand, within ybiS at 854.391 kb on - strand, within ybiS at 854.405 kb on + strand, within ybiS at 854.405 kb on + strand, within ybiS at 854.415 kb on + strand, within ybiS at 854.422 kb on - strand, within ybiS at 854.565 kb on + strand, within ybiS at 854.565 kb on + strand, within ybiS at 854.612 kb on + strand, within ybiS at 854.646 kb on + strand, within ybiS at 854.678 kb on - strand, within ybiS at 854.689 kb on - strand, within ybiS at 854.705 kb on + strand, within ybiS at 854.718 kb on - strand, within ybiS at 854.718 kb on - strand, within ybiS at 854.718 kb on - strand, within ybiS at 854.718 kb on - strand, within ybiS at 854.819 kb on + strand, within ybiS at 854.900 kb on - strand at 854.900 kb on - strand at 854.909 kb on - strand at 854.909 kb on - strand at 854.911 kb on + strand at 854.911 kb on + strand at 855.074 kb on - strand at 855.101 kb on - strand at 855.107 kb on + strand at 855.353 kb on - strand, within ybiT at 855.382 kb on + strand, within ybiT at 855.382 kb on + strand, within ybiT at 855.390 kb on - strand, within ybiT at 855.390 kb on - strand, within ybiT at 855.580 kb on - strand, within ybiT at 855.624 kb on - strand, within ybiT at 855.754 kb on - strand, within ybiT at 855.810 kb on + strand, within ybiT at 855.837 kb on + strand, within ybiT at 855.878 kb on - strand, within ybiT at 855.908 kb on - strand, within ybiT at 856.095 kb on - strand, within ybiT at 856.097 kb on - strand, within ybiT at 856.108 kb on + strand, within ybiT at 856.126 kb on - strand, within ybiT at 856.233 kb on + strand, within ybiT at 856.418 kb on - strand, within ybiT at 856.454 kb on - strand, within ybiT at 856.454 kb on - strand, within ybiT at 856.467 kb on + strand, within ybiT at 856.578 kb on + strand, within ybiT at 856.634 kb on + strand at 856.634 kb on + strand at 856.840 kb on + strand at 856.942 kb on - strand at 856.985 kb on + strand at 857.069 kb on + strand at 857.077 kb on - strand at 857.077 kb on - strand at 857.202 kb on - strand, within ybiU at 857.248 kb on + strand, within ybiU at 857.288 kb on - strand, within ybiU at 857.334 kb on + strand, within ybiU at 857.334 kb on + strand, within ybiU at 857.485 kb on - strand, within ybiU at 857.497 kb on - strand, within ybiU at 857.507 kb on - strand, within ybiU at 857.507 kb on - strand, within ybiU at 857.550 kb on - strand, within ybiU at 857.555 kb on - strand, within ybiU at 857.739 kb on - strand, within ybiU at 857.743 kb on - strand, within ybiU at 857.743 kb on - strand, within ybiU at 857.766 kb on + strand, within ybiU
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 854,212 - ybiS b0819 0.18 -0.2 854,264 + ybiS b0819 0.24 +0.1 854,264 + ybiS b0819 0.24 -0.6 854,285 - ybiS b0819 0.26 -0.2 854,290 - ybiS b0819 0.26 -1.0 854,391 - ybiS b0819 0.37 +1.8 854,405 + ybiS b0819 0.39 +2.0 854,405 + ybiS b0819 0.39 -0.0 854,415 + ybiS b0819 0.40 +0.5 854,422 - ybiS b0819 0.41 +0.5 854,565 + ybiS b0819 0.56 +0.8 854,565 + ybiS b0819 0.56 -0.0 854,612 + ybiS b0819 0.61 +0.3 854,646 + ybiS b0819 0.65 +1.0 854,678 - ybiS b0819 0.69 -0.1 854,689 - ybiS b0819 0.70 -0.0 854,705 + ybiS b0819 0.71 -0.7 854,718 - ybiS b0819 0.73 -0.5 854,718 - ybiS b0819 0.73 -0.6 854,718 - ybiS b0819 0.73 +0.0 854,718 - ybiS b0819 0.73 -1.0 854,819 + ybiS b0819 0.84 +0.4 854,900 - +1.7 854,900 - +0.2 854,909 - -2.5 854,909 - -0.2 854,911 + -0.5 854,911 + +0.5 855,074 - +1.0 855,101 - +0.5 855,107 + -0.6 855,353 - ybiT b0820 0.10 +0.5 855,382 + ybiT b0820 0.12 -0.5 855,382 + ybiT b0820 0.12 -0.0 855,390 - ybiT b0820 0.13 -0.2 855,390 - ybiT b0820 0.13 -1.6 855,580 - ybiT b0820 0.25 -0.0 855,624 - ybiT b0820 0.27 +0.3 855,754 - ybiT b0820 0.36 +0.8 855,810 + ybiT b0820 0.39 -0.0 855,837 + ybiT b0820 0.41 +0.8 855,878 - ybiT b0820 0.43 -0.6 855,908 - ybiT b0820 0.45 +1.1 856,095 - ybiT b0820 0.57 -0.4 856,097 - ybiT b0820 0.57 +0.5 856,108 + ybiT b0820 0.58 +0.0 856,126 - ybiT b0820 0.59 +0.3 856,233 + ybiT b0820 0.66 +1.3 856,418 - ybiT b0820 0.77 -0.5 856,454 - ybiT b0820 0.80 +0.5 856,454 - ybiT b0820 0.80 -0.1 856,467 + ybiT b0820 0.80 +0.2 856,578 + ybiT b0820 0.87 +0.2 856,634 + +0.4 856,634 + -0.9 856,840 + +0.2 856,942 - +0.2 856,985 + -0.5 857,069 + -0.5 857,077 - -0.6 857,077 - +1.5 857,202 - ybiU b0821 0.14 -1.3 857,248 + ybiU b0821 0.18 -1.0 857,288 - ybiU b0821 0.21 -0.5 857,334 + ybiU b0821 0.25 +1.1 857,334 + ybiU b0821 0.25 +2.0 857,485 - ybiU b0821 0.37 +1.4 857,497 - ybiU b0821 0.38 -0.5 857,507 - ybiU b0821 0.39 +0.2 857,507 - ybiU b0821 0.39 -0.9 857,550 - ybiU b0821 0.42 +1.0 857,555 - ybiU b0821 0.42 +0.3 857,739 - ybiU b0821 0.57 -0.0 857,743 - ybiU b0821 0.57 +0.5 857,743 - ybiU b0821 0.57 +0.1 857,766 + ybiU b0821 0.59 -1.6
Or see this region's nucleotide sequence