Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt moaB and moaC are separated by 2 nucleotides moaC and moaD overlap by 8 nucleotides moaD and moaE are separated by 1 nucleotides moaE and ybhL are separated by 136 nucleotides
b0782: moaB - molybdopterin biosynthesis protein B (NCBI), at 817,278 to 817,790
moaB
b0783: moaC - molybdenum cofactor biosynthesis protein C (NCBI), at 817,793 to 818,278
moaC
b0784: moaD - molybdopterin synthase, small subunit (NCBI), at 818,271 to 818,516
moaD
b0785: moaE - molybdopterin synthase, large subunit (NCBI), at 818,518 to 818,970
moaE
b0786: ybhL - predicted inner membrane protein (NCBI), at 819,107 to 819,811
ybhL
Position (kb) 818
819 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 817.818 kb on - strand at 817.818 kb on - strand at 817.852 kb on - strand, within moaC at 817.852 kb on - strand, within moaC at 817.872 kb on - strand, within moaC at 817.912 kb on + strand, within moaC at 817.912 kb on + strand, within moaC at 818.006 kb on - strand, within moaC at 818.012 kb on - strand, within moaC at 818.167 kb on - strand, within moaC at 818.211 kb on + strand, within moaC at 818.219 kb on - strand, within moaC at 818.219 kb on - strand, within moaC at 818.252 kb on + strand at 818.252 kb on + strand at 818.369 kb on + strand, within moaD at 818.455 kb on + strand, within moaD at 818.455 kb on + strand, within moaD at 818.566 kb on - strand, within moaE at 818.566 kb on - strand, within moaE at 818.566 kb on - strand, within moaE at 818.677 kb on - strand, within moaE at 818.687 kb on - strand, within moaE at 818.764 kb on - strand, within moaE at 818.892 kb on - strand, within moaE at 818.926 kb on + strand at 818.940 kb on - strand at 819.022 kb on + strand at 819.025 kb on - strand at 819.154 kb on + strand at 819.210 kb on + strand, within ybhL at 819.259 kb on + strand, within ybhL at 819.314 kb on + strand, within ybhL at 819.314 kb on + strand, within ybhL at 819.354 kb on + strand, within ybhL at 819.408 kb on + strand, within ybhL at 819.490 kb on + strand, within ybhL at 819.490 kb on + strand, within ybhL at 819.493 kb on + strand, within ybhL at 819.500 kb on + strand, within ybhL at 819.500 kb on + strand, within ybhL at 819.553 kb on - strand, within ybhL at 819.568 kb on - strand, within ybhL at 819.568 kb on - strand, within ybhL at 819.599 kb on + strand, within ybhL at 819.606 kb on + strand, within ybhL at 819.612 kb on - strand, within ybhL at 819.614 kb on + strand, within ybhL at 819.614 kb on + strand, within ybhL at 819.614 kb on + strand, within ybhL at 819.616 kb on + strand, within ybhL at 819.617 kb on + strand, within ybhL at 819.625 kb on - strand, within ybhL at 819.647 kb on - strand, within ybhL at 819.657 kb on + strand, within ybhL at 819.691 kb on + strand, within ybhL at 819.691 kb on + strand, within ybhL at 819.691 kb on + strand, within ybhL at 819.762 kb on + strand at 819.770 kb on - strand at 819.825 kb on + strand at 819.825 kb on + strand at 819.825 kb on + strand at 819.825 kb on + strand at 819.825 kb on + strand at 819.825 kb on + strand at 819.830 kb on + strand at 819.830 kb on + strand at 819.833 kb on - strand at 819.833 kb on - strand at 819.887 kb on + strand at 819.888 kb on + strand at 819.888 kb on + strand at 819.961 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 817,818 - +1.7 817,818 - +2.4 817,852 - moaC b0783 0.12 +2.3 817,852 - moaC b0783 0.12 +1.1 817,872 - moaC b0783 0.16 +1.7 817,912 + moaC b0783 0.24 -0.5 817,912 + moaC b0783 0.24 +2.4 818,006 - moaC b0783 0.44 +2.3 818,012 - moaC b0783 0.45 +1.5 818,167 - moaC b0783 0.77 +1.4 818,211 + moaC b0783 0.86 +2.2 818,219 - moaC b0783 0.88 +1.0 818,219 - moaC b0783 0.88 +1.6 818,252 + +1.2 818,252 + -0.1 818,369 + moaD b0784 0.40 +1.6 818,455 + moaD b0784 0.75 +1.3 818,455 + moaD b0784 0.75 +1.6 818,566 - moaE b0785 0.11 +1.5 818,566 - moaE b0785 0.11 +1.6 818,566 - moaE b0785 0.11 +1.1 818,677 - moaE b0785 0.35 +1.8 818,687 - moaE b0785 0.37 +3.0 818,764 - moaE b0785 0.54 +2.0 818,892 - moaE b0785 0.83 +1.8 818,926 + +1.2 818,940 - +1.9 819,022 + -1.8 819,025 - +0.3 819,154 + +0.2 819,210 + ybhL b0786 0.15 +0.5 819,259 + ybhL b0786 0.22 +0.3 819,314 + ybhL b0786 0.29 +0.5 819,314 + ybhL b0786 0.29 +0.7 819,354 + ybhL b0786 0.35 +1.8 819,408 + ybhL b0786 0.43 +0.3 819,490 + ybhL b0786 0.54 +1.2 819,490 + ybhL b0786 0.54 -0.7 819,493 + ybhL b0786 0.55 +0.2 819,500 + ybhL b0786 0.56 -0.9 819,500 + ybhL b0786 0.56 -1.8 819,553 - ybhL b0786 0.63 -0.1 819,568 - ybhL b0786 0.65 +0.5 819,568 - ybhL b0786 0.65 -0.5 819,599 + ybhL b0786 0.70 -0.5 819,606 + ybhL b0786 0.71 -0.3 819,612 - ybhL b0786 0.72 +0.8 819,614 + ybhL b0786 0.72 +0.6 819,614 + ybhL b0786 0.72 -0.7 819,614 + ybhL b0786 0.72 +0.9 819,616 + ybhL b0786 0.72 -1.0 819,617 + ybhL b0786 0.72 +0.4 819,625 - ybhL b0786 0.73 +0.3 819,647 - ybhL b0786 0.77 -0.8 819,657 + ybhL b0786 0.78 +0.5 819,691 + ybhL b0786 0.83 +0.9 819,691 + ybhL b0786 0.83 -0.1 819,691 + ybhL b0786 0.83 -1.7 819,762 + -2.5 819,770 - +0.3 819,825 + -2.9 819,825 + +0.5 819,825 + -0.2 819,825 + -0.3 819,825 + -0.5 819,825 + +0.7 819,830 + +0.1 819,830 + -0.8 819,833 - +0.4 819,833 - -0.9 819,887 + -0.5 819,888 + +0.7 819,888 + +0.9 819,961 + +0.7
Or see this region's nucleotide sequence