Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mhpC and mhpD are separated by 9 nucleotides mhpD and mhpF overlap by 4 nucleotides mhpF and mhpE overlap by 4 nucleotides
b0349: mhpC - 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase (VIMSS), at 370,448 to 371,329
mhpC
b0350: mhpD - 2-keto-4-pentenoate hydratase (VIMSS), at 371,339 to 372,148
mhpD
b0351: mhpF - acetaldehyde dehydrogenase (NCBI), at 372,145 to 373,095
mhpF
b0352: mhpE - 4-hydroxy-2-ketovalerate aldolase (NCBI), at 373,092 to 374,105
mhpE
Position (kb) 372
373
374 Strain fitness (log2 ratio) -1
0
1
2 at 371.194 kb on + strand, within mhpC at 371.196 kb on + strand, within mhpC at 371.237 kb on + strand, within mhpC at 371.247 kb on - strand at 371.280 kb on - strand at 371.325 kb on + strand at 371.331 kb on - strand at 371.331 kb on - strand at 371.331 kb on - strand at 371.360 kb on - strand at 371.472 kb on - strand, within mhpD at 371.489 kb on + strand, within mhpD at 371.511 kb on + strand, within mhpD at 371.522 kb on + strand, within mhpD at 371.522 kb on + strand, within mhpD at 371.530 kb on - strand, within mhpD at 371.753 kb on - strand, within mhpD at 371.791 kb on + strand, within mhpD at 371.815 kb on + strand, within mhpD at 371.936 kb on + strand, within mhpD at 371.943 kb on - strand, within mhpD at 371.982 kb on + strand, within mhpD at 371.996 kb on - strand, within mhpD at 371.996 kb on - strand, within mhpD at 371.999 kb on + strand, within mhpD at 371.999 kb on + strand, within mhpD at 372.009 kb on - strand, within mhpD at 372.028 kb on - strand, within mhpD at 372.181 kb on - strand at 372.181 kb on - strand at 372.191 kb on - strand at 372.191 kb on - strand at 372.193 kb on + strand at 372.193 kb on + strand at 372.226 kb on + strand at 372.226 kb on + strand at 372.271 kb on + strand, within mhpF at 372.307 kb on + strand, within mhpF at 372.308 kb on + strand, within mhpF at 372.308 kb on + strand, within mhpF at 372.308 kb on + strand, within mhpF at 372.619 kb on - strand, within mhpF at 372.648 kb on + strand, within mhpF at 372.779 kb on - strand, within mhpF at 372.779 kb on - strand, within mhpF at 372.793 kb on + strand, within mhpF at 372.814 kb on + strand, within mhpF at 372.820 kb on + strand, within mhpF at 372.844 kb on - strand, within mhpF at 372.854 kb on - strand, within mhpF at 372.903 kb on + strand, within mhpF at 372.991 kb on + strand, within mhpF at 372.991 kb on + strand, within mhpF at 373.062 kb on + strand at 373.130 kb on + strand at 373.130 kb on + strand at 373.393 kb on + strand, within mhpE at 373.429 kb on + strand, within mhpE at 373.429 kb on + strand, within mhpE at 373.437 kb on - strand, within mhpE at 373.552 kb on - strand, within mhpE at 373.576 kb on + strand, within mhpE at 373.576 kb on + strand, within mhpE at 373.640 kb on + strand, within mhpE at 373.640 kb on + strand, within mhpE at 373.736 kb on - strand, within mhpE at 373.787 kb on - strand, within mhpE at 373.787 kb on - strand, within mhpE at 373.857 kb on - strand, within mhpE at 373.857 kb on - strand, within mhpE at 373.912 kb on - strand, within mhpE
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 371,194 + mhpC b0349 0.85 +0.3 371,196 + mhpC b0349 0.85 -0.4 371,237 + mhpC b0349 0.89 +0.1 371,247 - +0.9 371,280 - +0.3 371,325 + -0.5 371,331 - +0.7 371,331 - +1.9 371,331 - +0.2 371,360 - +0.7 371,472 - mhpD b0350 0.16 +0.7 371,489 + mhpD b0350 0.19 -0.3 371,511 + mhpD b0350 0.21 -1.4 371,522 + mhpD b0350 0.23 +1.6 371,522 + mhpD b0350 0.23 -0.3 371,530 - mhpD b0350 0.24 +0.4 371,753 - mhpD b0350 0.51 -0.2 371,791 + mhpD b0350 0.56 +1.3 371,815 + mhpD b0350 0.59 -0.8 371,936 + mhpD b0350 0.74 +2.2 371,943 - mhpD b0350 0.75 -0.2 371,982 + mhpD b0350 0.79 -0.3 371,996 - mhpD b0350 0.81 +1.1 371,996 - mhpD b0350 0.81 -0.3 371,999 + mhpD b0350 0.81 -0.3 371,999 + mhpD b0350 0.81 -0.1 372,009 - mhpD b0350 0.83 +0.2 372,028 - mhpD b0350 0.85 +0.0 372,181 - +0.2 372,181 - +1.0 372,191 - -0.3 372,191 - +0.7 372,193 + -0.8 372,193 + -0.2 372,226 + +0.7 372,226 + +1.0 372,271 + mhpF b0351 0.13 +0.3 372,307 + mhpF b0351 0.17 +0.1 372,308 + mhpF b0351 0.17 -0.2 372,308 + mhpF b0351 0.17 +0.7 372,308 + mhpF b0351 0.17 +2.2 372,619 - mhpF b0351 0.50 +1.3 372,648 + mhpF b0351 0.53 +0.1 372,779 - mhpF b0351 0.67 +0.7 372,779 - mhpF b0351 0.67 -1.5 372,793 + mhpF b0351 0.68 +0.2 372,814 + mhpF b0351 0.70 +0.2 372,820 + mhpF b0351 0.71 +0.2 372,844 - mhpF b0351 0.74 -0.1 372,854 - mhpF b0351 0.75 +0.6 372,903 + mhpF b0351 0.80 +0.1 372,991 + mhpF b0351 0.89 +1.2 372,991 + mhpF b0351 0.89 -0.9 373,062 + +0.1 373,130 + +0.7 373,130 + +0.7 373,393 + mhpE b0352 0.30 +0.0 373,429 + mhpE b0352 0.33 +0.4 373,429 + mhpE b0352 0.33 +0.4 373,437 - mhpE b0352 0.34 +0.7 373,552 - mhpE b0352 0.45 +1.8 373,576 + mhpE b0352 0.48 -0.1 373,576 + mhpE b0352 0.48 -0.3 373,640 + mhpE b0352 0.54 -0.3 373,640 + mhpE b0352 0.54 +0.1 373,736 - mhpE b0352 0.64 +0.4 373,787 - mhpE b0352 0.69 -0.1 373,787 - mhpE b0352 0.69 +0.4 373,857 - mhpE b0352 0.75 +0.4 373,857 - mhpE b0352 0.75 +0.0 373,912 - mhpE b0352 0.81 +0.8
Or see this region's nucleotide sequence