Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fhuA and fhuC are separated by 50 nucleotides fhuC and fhuD overlap by 1 nucleotides fhuD and fhuB overlap by 4 nucleotides
b0150: fhuA - ferrichrome outer membrane transporter (NCBI), at 167,484 to 169,727
fhuA
b0151: fhuC - iron-hydroxamate transporter subunit (NCBI), at 169,778 to 170,575
fhuC
b0152: fhuD - iron-hydroxamate transporter subunit (NCBI), at 170,575 to 171,465
fhuD
b0153: fhuB - fused iron-hydroxamate transporter subunits of ABC superfamily: membrane components (NCBI), at 171,462 to 173,444
fhuB
Position (kb) 170
171
172 Strain fitness (log2 ratio) -1
0
1
2
3 at 169.594 kb on - strand at 169.620 kb on + strand at 169.629 kb on - strand at 169.635 kb on - strand at 169.635 kb on - strand at 169.639 kb on - strand at 169.684 kb on - strand at 169.827 kb on - strand at 169.926 kb on + strand, within fhuC at 169.926 kb on + strand, within fhuC at 169.926 kb on + strand, within fhuC at 169.929 kb on - strand, within fhuC at 169.965 kb on + strand, within fhuC at 169.966 kb on + strand, within fhuC at 169.966 kb on + strand, within fhuC at 169.989 kb on + strand, within fhuC at 170.210 kb on + strand, within fhuC at 170.210 kb on + strand, within fhuC at 170.250 kb on + strand, within fhuC at 170.250 kb on - strand, within fhuC at 170.255 kb on + strand, within fhuC at 170.257 kb on + strand, within fhuC at 170.390 kb on - strand, within fhuC at 170.390 kb on - strand, within fhuC at 170.395 kb on - strand, within fhuC at 170.654 kb on - strand at 170.671 kb on + strand, within fhuD at 170.671 kb on + strand, within fhuD at 170.689 kb on - strand, within fhuD at 170.739 kb on + strand, within fhuD at 170.739 kb on + strand, within fhuD at 170.755 kb on - strand, within fhuD at 170.755 kb on - strand, within fhuD at 170.775 kb on + strand, within fhuD at 170.831 kb on + strand, within fhuD at 170.869 kb on + strand, within fhuD at 170.869 kb on + strand, within fhuD at 170.895 kb on - strand, within fhuD at 170.895 kb on - strand, within fhuD at 170.972 kb on - strand, within fhuD at 171.065 kb on + strand, within fhuD at 171.199 kb on + strand, within fhuD at 171.199 kb on + strand, within fhuD at 171.219 kb on + strand, within fhuD at 171.225 kb on + strand, within fhuD at 171.225 kb on + strand, within fhuD at 171.228 kb on + strand, within fhuD at 171.228 kb on + strand, within fhuD at 171.238 kb on - strand, within fhuD at 171.238 kb on - strand, within fhuD at 171.307 kb on - strand, within fhuD at 171.336 kb on + strand, within fhuD at 171.336 kb on + strand, within fhuD at 171.423 kb on + strand at 171.423 kb on - strand at 171.423 kb on - strand at 171.506 kb on + strand at 171.514 kb on - strand at 171.533 kb on - strand at 171.678 kb on + strand, within fhuB at 171.678 kb on + strand, within fhuB at 171.680 kb on + strand, within fhuB at 171.680 kb on + strand, within fhuB at 171.680 kb on - strand, within fhuB at 171.680 kb on - strand, within fhuB at 171.695 kb on + strand, within fhuB at 171.742 kb on + strand, within fhuB at 171.794 kb on + strand, within fhuB at 171.867 kb on + strand, within fhuB at 171.910 kb on + strand, within fhuB at 171.910 kb on + strand, within fhuB at 172.063 kb on + strand, within fhuB at 172.088 kb on - strand, within fhuB at 172.202 kb on + strand, within fhuB at 172.218 kb on - strand, within fhuB at 172.222 kb on - strand, within fhuB at 172.261 kb on + strand, within fhuB at 172.281 kb on + strand, within fhuB at 172.281 kb on + strand, within fhuB at 172.289 kb on + strand, within fhuB at 172.290 kb on - strand, within fhuB at 172.293 kb on + strand, within fhuB at 172.293 kb on + strand, within fhuB at 172.314 kb on + strand, within fhuB at 172.369 kb on - strand, within fhuB
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 169,594 - -0.7 169,620 + -0.9 169,629 - +0.4 169,635 - -0.5 169,635 - +1.4 169,639 - +0.7 169,684 - +0.5 169,827 - +0.1 169,926 + fhuC b0151 0.19 -1.0 169,926 + fhuC b0151 0.19 +0.4 169,926 + fhuC b0151 0.19 +0.8 169,929 - fhuC b0151 0.19 +0.1 169,965 + fhuC b0151 0.23 +0.7 169,966 + fhuC b0151 0.24 +1.7 169,966 + fhuC b0151 0.24 +0.6 169,989 + fhuC b0151 0.26 -0.4 170,210 + fhuC b0151 0.54 +0.7 170,210 + fhuC b0151 0.54 +0.9 170,250 + fhuC b0151 0.59 -1.1 170,250 - fhuC b0151 0.59 +1.2 170,255 + fhuC b0151 0.60 +0.9 170,257 + fhuC b0151 0.60 -1.0 170,390 - fhuC b0151 0.77 +0.7 170,390 - fhuC b0151 0.77 -0.5 170,395 - fhuC b0151 0.77 +0.2 170,654 - +1.7 170,671 + fhuD b0152 0.11 +0.4 170,671 + fhuD b0152 0.11 +0.9 170,689 - fhuD b0152 0.13 +0.6 170,739 + fhuD b0152 0.18 +0.5 170,739 + fhuD b0152 0.18 +1.2 170,755 - fhuD b0152 0.20 +1.9 170,755 - fhuD b0152 0.20 +0.6 170,775 + fhuD b0152 0.22 +0.9 170,831 + fhuD b0152 0.29 +0.9 170,869 + fhuD b0152 0.33 -0.9 170,869 + fhuD b0152 0.33 +0.6 170,895 - fhuD b0152 0.36 +0.0 170,895 - fhuD b0152 0.36 +0.6 170,972 - fhuD b0152 0.45 -0.6 171,065 + fhuD b0152 0.55 +0.5 171,199 + fhuD b0152 0.70 +0.9 171,199 + fhuD b0152 0.70 +0.1 171,219 + fhuD b0152 0.72 -0.4 171,225 + fhuD b0152 0.73 +0.5 171,225 + fhuD b0152 0.73 -0.5 171,228 + fhuD b0152 0.73 +0.3 171,228 + fhuD b0152 0.73 +1.3 171,238 - fhuD b0152 0.74 +2.8 171,238 - fhuD b0152 0.74 +3.4 171,307 - fhuD b0152 0.82 +0.9 171,336 + fhuD b0152 0.85 -1.5 171,336 + fhuD b0152 0.85 +0.3 171,423 + -1.7 171,423 - +0.7 171,423 - -0.7 171,506 + +0.1 171,514 - +0.3 171,533 - +0.0 171,678 + fhuB b0153 0.11 +1.0 171,678 + fhuB b0153 0.11 -1.0 171,680 + fhuB b0153 0.11 +0.4 171,680 + fhuB b0153 0.11 +0.0 171,680 - fhuB b0153 0.11 -0.1 171,680 - fhuB b0153 0.11 +0.6 171,695 + fhuB b0153 0.12 +0.4 171,742 + fhuB b0153 0.14 -0.4 171,794 + fhuB b0153 0.17 -0.6 171,867 + fhuB b0153 0.20 +0.0 171,910 + fhuB b0153 0.23 -1.1 171,910 + fhuB b0153 0.23 -1.1 172,063 + fhuB b0153 0.30 -1.0 172,088 - fhuB b0153 0.32 +0.1 172,202 + fhuB b0153 0.37 +0.7 172,218 - fhuB b0153 0.38 +0.9 172,222 - fhuB b0153 0.38 -0.1 172,261 + fhuB b0153 0.40 +1.9 172,281 + fhuB b0153 0.41 +0.2 172,281 + fhuB b0153 0.41 +1.2 172,289 + fhuB b0153 0.42 -0.1 172,290 - fhuB b0153 0.42 +0.9 172,293 + fhuB b0153 0.42 +0.3 172,293 + fhuB b0153 0.42 +0.3 172,314 + fhuB b0153 0.43 +0.2 172,369 - fhuB b0153 0.46 -0.2
Or see this region's nucleotide sequence