Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrC and yaaX are separated by 213 nucleotides yaaX and yaaA are separated by 152 nucleotides yaaA and yaaJ are separated by 69 nucleotides
b0004: thrC - threonine synthase (NCBI), at 3,734 to 5,020
thrC
b0005: yaaX - hypothetical protein (NCBI), at 5,234 to 5,530
yaaX
b0006: yaaA - hypothetical protein (NCBI), at 5,683 to 6,459
yaaA
b0007: yaaJ - predicted transporter (NCBI), at 6,529 to 7,959
yaaJ
Position (kb) 5
6 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4.253 kb on - strand, within thrC at 4.253 kb on - strand, within thrC at 4.464 kb on - strand, within thrC at 4.466 kb on + strand, within thrC at 4.619 kb on + strand, within thrC at 4.721 kb on + strand, within thrC at 4.729 kb on + strand, within thrC at 4.729 kb on - strand, within thrC at 4.729 kb on - strand, within thrC at 4.755 kb on - strand, within thrC at 4.755 kb on - strand, within thrC at 4.755 kb on - strand, within thrC at 4.771 kb on + strand, within thrC at 4.771 kb on + strand, within thrC at 4.779 kb on - strand, within thrC at 4.779 kb on - strand, within thrC at 4.796 kb on + strand, within thrC at 4.875 kb on + strand, within thrC at 4.932 kb on + strand at 4.932 kb on + strand at 4.993 kb on + strand at 5.238 kb on + strand at 5.241 kb on + strand at 5.241 kb on + strand at 5.273 kb on + strand, within yaaX at 5.273 kb on + strand, within yaaX at 5.280 kb on - strand, within yaaX at 5.283 kb on + strand, within yaaX at 5.285 kb on + strand, within yaaX at 5.358 kb on - strand, within yaaX at 5.470 kb on + strand, within yaaX at 5.470 kb on + strand, within yaaX at 5.639 kb on - strand at 5.699 kb on - strand at 5.699 kb on - strand at 5.788 kb on - strand, within yaaA at 5.788 kb on - strand, within yaaA at 5.835 kb on - strand, within yaaA at 5.879 kb on - strand, within yaaA at 5.891 kb on - strand, within yaaA at 5.898 kb on + strand, within yaaA at 5.958 kb on + strand, within yaaA at 5.976 kb on + strand, within yaaA at 5.976 kb on + strand, within yaaA at 5.995 kb on + strand, within yaaA at 6.005 kb on - strand, within yaaA at 6.027 kb on - strand, within yaaA at 6.071 kb on - strand, within yaaA at 6.073 kb on - strand, within yaaA at 6.073 kb on - strand, within yaaA at 6.087 kb on + strand, within yaaA at 6.087 kb on + strand, within yaaA at 6.108 kb on + strand, within yaaA at 6.108 kb on + strand, within yaaA at 6.278 kb on + strand, within yaaA at 6.278 kb on + strand, within yaaA at 6.285 kb on - strand, within yaaA at 6.288 kb on - strand, within yaaA at 6.288 kb on - strand, within yaaA at 6.317 kb on + strand, within yaaA at 6.317 kb on + strand, within yaaA at 6.317 kb on - strand, within yaaA at 6.317 kb on - strand, within yaaA at 6.348 kb on + strand, within yaaA at 6.348 kb on + strand, within yaaA at 6.348 kb on + strand, within yaaA at 6.355 kb on + strand, within yaaA at 6.420 kb on + strand at 6.456 kb on + strand at 6.456 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 4,253 - thrC b0004 0.40 +0.6 4,253 - thrC b0004 0.40 -1.1 4,464 - thrC b0004 0.57 +0.6 4,466 + thrC b0004 0.57 +2.7 4,619 + thrC b0004 0.69 +0.5 4,721 + thrC b0004 0.77 +0.9 4,729 + thrC b0004 0.77 +0.4 4,729 - thrC b0004 0.77 +0.2 4,729 - thrC b0004 0.77 +1.4 4,755 - thrC b0004 0.79 +0.1 4,755 - thrC b0004 0.79 +0.9 4,755 - thrC b0004 0.79 +0.1 4,771 + thrC b0004 0.81 +0.1 4,771 + thrC b0004 0.81 +0.6 4,779 - thrC b0004 0.81 -0.6 4,779 - thrC b0004 0.81 +0.1 4,796 + thrC b0004 0.83 +0.6 4,875 + thrC b0004 0.89 +0.9 4,932 + -1.7 4,932 + +0.7 4,993 + -1.0 5,238 + -1.0 5,241 + +1.2 5,241 + +0.1 5,273 + yaaX b0005 0.13 +0.9 5,273 + yaaX b0005 0.13 +1.0 5,280 - yaaX b0005 0.15 +2.2 5,283 + yaaX b0005 0.16 +0.5 5,285 + yaaX b0005 0.17 +0.1 5,358 - yaaX b0005 0.42 -0.6 5,470 + yaaX b0005 0.79 +0.4 5,470 + yaaX b0005 0.79 +0.2 5,639 - +0.3 5,699 - -1.5 5,699 - -0.3 5,788 - yaaA b0006 0.14 +0.6 5,788 - yaaA b0006 0.14 -0.6 5,835 - yaaA b0006 0.20 +0.1 5,879 - yaaA b0006 0.25 +0.0 5,891 - yaaA b0006 0.27 +0.7 5,898 + yaaA b0006 0.28 +0.6 5,958 + yaaA b0006 0.35 +0.1 5,976 + yaaA b0006 0.38 -0.7 5,976 + yaaA b0006 0.38 -1.7 5,995 + yaaA b0006 0.40 +0.2 6,005 - yaaA b0006 0.41 -0.6 6,027 - yaaA b0006 0.44 +0.6 6,071 - yaaA b0006 0.50 +0.5 6,073 - yaaA b0006 0.50 +0.9 6,073 - yaaA b0006 0.50 -0.4 6,087 + yaaA b0006 0.52 +2.9 6,087 + yaaA b0006 0.52 -1.5 6,108 + yaaA b0006 0.55 -2.1 6,108 + yaaA b0006 0.55 +0.3 6,278 + yaaA b0006 0.77 +0.3 6,278 + yaaA b0006 0.77 -0.3 6,285 - yaaA b0006 0.77 -0.7 6,288 - yaaA b0006 0.78 +0.4 6,288 - yaaA b0006 0.78 +0.4 6,317 + yaaA b0006 0.82 -1.0 6,317 + yaaA b0006 0.82 +0.2 6,317 - yaaA b0006 0.82 +0.7 6,317 - yaaA b0006 0.82 +0.7 6,348 + yaaA b0006 0.86 -0.4 6,348 + yaaA b0006 0.86 +1.1 6,348 + yaaA b0006 0.86 -0.0 6,355 + yaaA b0006 0.86 -0.1 6,420 + +0.6 6,456 + +1.9 6,456 + +1.4
Or see this region's nucleotide sequence