Experiment: 150_an_nonmag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS11815 and AMB_RS11820 are separated by 69 nucleotides AMB_RS11820 and AMB_RS11825 are separated by 31 nucleotides AMB_RS11825 and AMB_RS11830 are separated by 18 nucleotides AMB_RS11830 and AMB_RS11835 overlap by 29 nucleotides
AMB_RS11815: AMB_RS11815 - serine hydroxymethyltransferase, at 2,545,611 to 2,546,894
_RS11815
AMB_RS11820: AMB_RS11820 - transcriptional regulator NrdR, at 2,546,964 to 2,547,425
_RS11820
AMB_RS11825: AMB_RS11825 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 2,547,457 to 2,548,533
_RS11825
AMB_RS11830: AMB_RS11830 - riboflavin synthase, at 2,548,552 to 2,549,144
_RS11830
AMB_RS11835: AMB_RS11835 - 3,4-dihydroxy-2-butanone-4-phosphate synthase, at 2,549,116 to 2,550,264
_RS11835
Position (kb)
2547
2548
2549 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2546.886 kb on + strand at 2546.887 kb on - strand at 2546.887 kb on - strand at 2546.892 kb on + strand at 2546.893 kb on - strand at 2547.026 kb on + strand, within AMB_RS11820 at 2547.185 kb on - strand, within AMB_RS11820 at 2547.333 kb on + strand, within AMB_RS11820 at 2547.333 kb on + strand, within AMB_RS11820 at 2548.529 kb on + strand at 2548.531 kb on + strand at 2548.531 kb on + strand at 2549.170 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_an_nonmag_1 remove 2,546,886 + -1.5 2,546,887 - -2.2 2,546,887 - -0.8 2,546,892 + +1.5 2,546,893 - -4.3 2,547,026 + AMB_RS11820 0.13 +1.5 2,547,185 - AMB_RS11820 0.48 -4.1 2,547,333 + AMB_RS11820 0.80 -1.4 2,547,333 + AMB_RS11820 0.80 -1.6 2,548,529 + +0.4 2,548,531 + -1.5 2,548,531 + -2.7 2,549,170 + -0.3
Or see this region's nucleotide sequence