Experiment: 150_an_nonmag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS09350 and AMB_RS09355 overlap by 4 nucleotides AMB_RS09355 and AMB_RS09360 are separated by 23 nucleotides AMB_RS09360 and AMB_RS09365 are separated by 14 nucleotides
AMB_RS09350: AMB_RS09350 - SufS family cysteine desulfurase, at 1,990,714 to 1,992,498
_RS09350
AMB_RS09355: AMB_RS09355 - hypothetical protein, at 1,992,495 to 1,993,448
_RS09355
AMB_RS09360: AMB_RS09360 - DUF2325 domain-containing protein, at 1,993,472 to 1,993,807
_RS09360
AMB_RS09365: AMB_RS09365 - serine O-acetyltransferase, at 1,993,822 to 1,994,709
_RS09365
Position (kb)
1992
1993
1994 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1991.539 kb on + strand, within AMB_RS09350 at 1991.540 kb on - strand, within AMB_RS09350 at 1991.801 kb on + strand, within AMB_RS09350 at 1992.079 kb on + strand, within AMB_RS09350 at 1992.079 kb on + strand, within AMB_RS09350 at 1992.080 kb on - strand, within AMB_RS09350 at 1992.089 kb on - strand, within AMB_RS09350 at 1992.089 kb on - strand, within AMB_RS09350 at 1992.514 kb on + strand at 1992.523 kb on + strand at 1992.524 kb on - strand at 1992.524 kb on - strand at 1992.524 kb on - strand at 1992.524 kb on - strand at 1992.589 kb on + strand at 1992.590 kb on - strand at 1992.590 kb on - strand at 1992.604 kb on + strand, within AMB_RS09355 at 1992.605 kb on - strand, within AMB_RS09355 at 1993.048 kb on + strand, within AMB_RS09355 at 1993.049 kb on - strand, within AMB_RS09355 at 1993.120 kb on + strand, within AMB_RS09355 at 1993.121 kb on - strand, within AMB_RS09355 at 1993.121 kb on - strand, within AMB_RS09355 at 1993.121 kb on - strand, within AMB_RS09355 at 1993.121 kb on - strand, within AMB_RS09355 at 1993.297 kb on + strand, within AMB_RS09355 at 1993.298 kb on - strand, within AMB_RS09355 at 1993.547 kb on - strand, within AMB_RS09360 at 1993.547 kb on - strand, within AMB_RS09360 at 1993.755 kb on + strand, within AMB_RS09360 at 1993.755 kb on + strand, within AMB_RS09360 at 1993.756 kb on - strand, within AMB_RS09360 at 1993.756 kb on - strand, within AMB_RS09360 at 1993.756 kb on - strand, within AMB_RS09360 at 1993.756 kb on - strand, within AMB_RS09360 at 1993.832 kb on + strand at 1993.833 kb on - strand at 1993.833 kb on - strand at 1993.833 kb on - strand at 1993.833 kb on - strand at 1993.847 kb on + strand at 1993.914 kb on + strand, within AMB_RS09365 at 1994.249 kb on + strand, within AMB_RS09365 at 1994.250 kb on - strand, within AMB_RS09365 at 1994.331 kb on - strand, within AMB_RS09365 at 1994.331 kb on - strand, within AMB_RS09365
Per-strain Table
Position Strand Gene LocusTag Fraction 150_an_nonmag_1 remove 1,991,539 + AMB_RS09350 0.46 +0.3 1,991,540 - AMB_RS09350 0.46 -2.1 1,991,801 + AMB_RS09350 0.61 +0.7 1,992,079 + AMB_RS09350 0.76 +0.6 1,992,079 + AMB_RS09350 0.76 +0.3 1,992,080 - AMB_RS09350 0.77 +3.1 1,992,089 - AMB_RS09350 0.77 -2.8 1,992,089 - AMB_RS09350 0.77 +0.4 1,992,514 + -2.0 1,992,523 + -0.7 1,992,524 - -0.9 1,992,524 - -0.1 1,992,524 - -0.0 1,992,524 - -0.4 1,992,589 + -0.4 1,992,590 - +0.4 1,992,590 - -0.9 1,992,604 + AMB_RS09355 0.11 -0.1 1,992,605 - AMB_RS09355 0.12 -2.0 1,993,048 + AMB_RS09355 0.58 -0.1 1,993,049 - AMB_RS09355 0.58 -1.1 1,993,120 + AMB_RS09355 0.66 +1.4 1,993,121 - AMB_RS09355 0.66 +0.4 1,993,121 - AMB_RS09355 0.66 -2.0 1,993,121 - AMB_RS09355 0.66 -2.8 1,993,121 - AMB_RS09355 0.66 -0.7 1,993,297 + AMB_RS09355 0.84 +1.4 1,993,298 - AMB_RS09355 0.84 -0.6 1,993,547 - AMB_RS09360 0.22 -2.7 1,993,547 - AMB_RS09360 0.22 +1.0 1,993,755 + AMB_RS09360 0.84 -3.0 1,993,755 + AMB_RS09360 0.84 -0.3 1,993,756 - AMB_RS09360 0.85 +0.1 1,993,756 - AMB_RS09360 0.85 -1.1 1,993,756 - AMB_RS09360 0.85 -0.6 1,993,756 - AMB_RS09360 0.85 +0.2 1,993,832 + -1.7 1,993,833 - -2.4 1,993,833 - -2.9 1,993,833 - -1.2 1,993,833 - -3.8 1,993,847 + -1.0 1,993,914 + AMB_RS09365 0.10 -1.4 1,994,249 + AMB_RS09365 0.48 +0.4 1,994,250 - AMB_RS09365 0.48 -1.1 1,994,331 - AMB_RS09365 0.57 -1.9 1,994,331 - AMB_RS09365 0.57 -0.6
Or see this region's nucleotide sequence