Experiment: Tetracycline 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygjK and fadH are separated by 425 nucleotides fadH and ygjM are separated by 44 nucleotides ygjM and ygjN overlap by 4 nucleotides
b3080: ygjK - predicted glycosyl hydrolase (NCBI), at 3,226,910 to 3,229,261
ygjK
b3081: fadH - 2,4-dienoyl-CoA reductase, NADH and FMN-linked (NCBI), at 3,229,687 to 3,231,705
fadH
b3082: ygjM - predicted DNA-binding transcriptional regulator (NCBI), at 3,231,750 to 3,232,166
ygjM
b3083: ygjN - hypothetical protein (NCBI), at 3,232,163 to 3,232,477
ygjN
Position (kb)
3229
3230
3231
3232 Strain fitness (log2 ratio)
-1
0
1
2 at 3228.714 kb on + strand, within ygjK at 3228.714 kb on + strand, within ygjK at 3228.726 kb on + strand, within ygjK at 3228.771 kb on + strand, within ygjK at 3228.771 kb on + strand, within ygjK at 3228.784 kb on - strand, within ygjK at 3228.797 kb on - strand, within ygjK at 3228.899 kb on + strand, within ygjK at 3228.918 kb on + strand, within ygjK at 3228.918 kb on + strand, within ygjK at 3228.939 kb on + strand, within ygjK at 3229.131 kb on - strand at 3229.170 kb on - strand at 3229.286 kb on + strand at 3229.356 kb on + strand at 3229.391 kb on + strand at 3229.491 kb on + strand at 3229.491 kb on + strand at 3229.560 kb on + strand at 3229.560 kb on + strand at 3229.595 kb on + strand at 3229.598 kb on - strand at 3229.598 kb on - strand at 3229.611 kb on - strand at 3229.757 kb on + strand at 3229.820 kb on - strand at 3229.906 kb on - strand, within fadH at 3229.927 kb on + strand, within fadH at 3230.012 kb on + strand, within fadH at 3230.012 kb on + strand, within fadH at 3230.026 kb on - strand, within fadH at 3230.139 kb on - strand, within fadH at 3230.144 kb on + strand, within fadH at 3230.159 kb on + strand, within fadH at 3230.159 kb on + strand, within fadH at 3230.167 kb on - strand, within fadH at 3230.174 kb on + strand, within fadH at 3230.213 kb on + strand, within fadH at 3230.213 kb on + strand, within fadH at 3230.216 kb on + strand, within fadH at 3230.290 kb on + strand, within fadH at 3230.323 kb on + strand, within fadH at 3230.442 kb on + strand, within fadH at 3230.466 kb on + strand, within fadH at 3230.466 kb on + strand, within fadH at 3230.709 kb on - strand, within fadH at 3230.709 kb on - strand, within fadH at 3230.714 kb on - strand, within fadH at 3230.714 kb on - strand, within fadH at 3230.714 kb on - strand, within fadH at 3230.922 kb on + strand, within fadH at 3230.928 kb on - strand, within fadH at 3231.019 kb on + strand, within fadH at 3231.139 kb on + strand, within fadH at 3231.163 kb on - strand, within fadH at 3231.199 kb on - strand, within fadH at 3231.267 kb on + strand, within fadH at 3231.267 kb on + strand, within fadH at 3231.356 kb on + strand, within fadH at 3231.399 kb on - strand, within fadH at 3231.427 kb on - strand, within fadH at 3231.428 kb on + strand, within fadH at 3231.428 kb on + strand, within fadH at 3231.428 kb on + strand, within fadH at 3231.428 kb on + strand, within fadH at 3231.504 kb on - strand at 3231.530 kb on + strand at 3231.530 kb on + strand at 3231.547 kb on + strand at 3231.552 kb on + strand at 3231.643 kb on + strand at 3231.673 kb on + strand at 3231.812 kb on - strand, within ygjM at 3231.855 kb on - strand, within ygjM at 3231.856 kb on - strand, within ygjM at 3231.892 kb on - strand, within ygjM at 3231.892 kb on - strand, within ygjM at 3232.179 kb on + strand at 3232.179 kb on + strand at 3232.181 kb on + strand at 3232.186 kb on + strand at 3232.189 kb on - strand at 3232.233 kb on + strand, within ygjN at 3232.267 kb on + strand, within ygjN at 3232.272 kb on - strand, within ygjN at 3232.302 kb on + strand, within ygjN at 3232.394 kb on + strand, within ygjN at 3232.416 kb on + strand, within ygjN at 3232.465 kb on - strand at 3232.465 kb on - strand at 3232.465 kb on - strand at 3232.471 kb on + strand at 3232.603 kb on + strand at 3232.603 kb on + strand at 3232.704 kb on - strand at 3232.704 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0008 mg/ml remove 3,228,714 + ygjK b3080 0.77 -0.6 3,228,714 + ygjK b3080 0.77 +0.6 3,228,726 + ygjK b3080 0.77 -0.4 3,228,771 + ygjK b3080 0.79 -0.1 3,228,771 + ygjK b3080 0.79 +1.4 3,228,784 - ygjK b3080 0.80 -0.1 3,228,797 - ygjK b3080 0.80 -1.0 3,228,899 + ygjK b3080 0.85 -0.5 3,228,918 + ygjK b3080 0.85 -0.5 3,228,918 + ygjK b3080 0.85 -0.4 3,228,939 + ygjK b3080 0.86 +0.4 3,229,131 - +0.4 3,229,170 - -0.1 3,229,286 + +1.1 3,229,356 + +0.7 3,229,391 + +0.5 3,229,491 + +1.7 3,229,491 + +0.7 3,229,560 + +0.7 3,229,560 + -0.2 3,229,595 + +0.5 3,229,598 - -0.2 3,229,598 - +0.2 3,229,611 - +0.4 3,229,757 + +0.2 3,229,820 - +0.0 3,229,906 - fadH b3081 0.11 -0.6 3,229,927 + fadH b3081 0.12 +0.3 3,230,012 + fadH b3081 0.16 +0.5 3,230,012 + fadH b3081 0.16 -1.3 3,230,026 - fadH b3081 0.17 +0.2 3,230,139 - fadH b3081 0.22 -0.4 3,230,144 + fadH b3081 0.23 -0.8 3,230,159 + fadH b3081 0.23 +0.5 3,230,159 + fadH b3081 0.23 -0.5 3,230,167 - fadH b3081 0.24 -0.0 3,230,174 + fadH b3081 0.24 -0.1 3,230,213 + fadH b3081 0.26 +1.5 3,230,213 + fadH b3081 0.26 -1.1 3,230,216 + fadH b3081 0.26 -0.1 3,230,290 + fadH b3081 0.30 +0.5 3,230,323 + fadH b3081 0.32 +0.1 3,230,442 + fadH b3081 0.37 -0.8 3,230,466 + fadH b3081 0.39 -0.3 3,230,466 + fadH b3081 0.39 -0.0 3,230,709 - fadH b3081 0.51 +1.0 3,230,709 - fadH b3081 0.51 -0.6 3,230,714 - fadH b3081 0.51 +0.2 3,230,714 - fadH b3081 0.51 -1.2 3,230,714 - fadH b3081 0.51 +0.7 3,230,922 + fadH b3081 0.61 -1.0 3,230,928 - fadH b3081 0.61 -0.2 3,231,019 + fadH b3081 0.66 -1.1 3,231,139 + fadH b3081 0.72 -1.0 3,231,163 - fadH b3081 0.73 +0.5 3,231,199 - fadH b3081 0.75 -0.1 3,231,267 + fadH b3081 0.78 -0.3 3,231,267 + fadH b3081 0.78 -0.2 3,231,356 + fadH b3081 0.83 -0.6 3,231,399 - fadH b3081 0.85 -0.7 3,231,427 - fadH b3081 0.86 +0.0 3,231,428 + fadH b3081 0.86 -1.1 3,231,428 + fadH b3081 0.86 -1.0 3,231,428 + fadH b3081 0.86 -0.5 3,231,428 + fadH b3081 0.86 +1.7 3,231,504 - -0.7 3,231,530 + +0.9 3,231,530 + +0.2 3,231,547 + -0.9 3,231,552 + +0.0 3,231,643 + +0.3 3,231,673 + +0.5 3,231,812 - ygjM b3082 0.15 -1.1 3,231,855 - ygjM b3082 0.25 +0.0 3,231,856 - ygjM b3082 0.25 +0.0 3,231,892 - ygjM b3082 0.34 -1.3 3,231,892 - ygjM b3082 0.34 -1.0 3,232,179 + +0.2 3,232,179 + -0.3 3,232,181 + +1.0 3,232,186 + -0.5 3,232,189 - -0.7 3,232,233 + ygjN b3083 0.22 +0.2 3,232,267 + ygjN b3083 0.33 +0.0 3,232,272 - ygjN b3083 0.35 +1.0 3,232,302 + ygjN b3083 0.44 +0.3 3,232,394 + ygjN b3083 0.73 +0.2 3,232,416 + ygjN b3083 0.80 +0.8 3,232,465 - -1.0 3,232,465 - +1.0 3,232,465 - -0.5 3,232,471 + -0.5 3,232,603 + -0.3 3,232,603 + +2.0 3,232,704 - +0.5 3,232,704 - +0.2
Or see this region's nucleotide sequence