Experiment: Tetracycline 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rsxA and rsxB overlap by 1 nucleotides rsxB and rsxC overlap by 8 nucleotides rsxC and rsxD are separated by 0 nucleotides
b1627: rsxA - Na(+)-translocating NADH-quinone reductase subunit E (NCBI), at 1,703,791 to 1,704,372
rsxA
b1628: rsxB - electron transport complex protein RnfB (NCBI), at 1,704,372 to 1,704,950
rsxB
b1629: rsxC - electron transport complex protein RnfC (NCBI), at 1,704,943 to 1,707,165
rsxC
b1630: rsxD - electron transport complex protein RnfD (NCBI), at 1,707,166 to 1,708,224
rsxD
Position (kb)
1704
1705
1706
1707
1708 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1704.048 kb on + strand, within rsxA at 1704.090 kb on - strand, within rsxA at 1704.235 kb on - strand, within rsxA at 1704.376 kb on + strand at 1704.376 kb on + strand at 1704.377 kb on - strand at 1704.525 kb on - strand, within rsxB at 1704.772 kb on + strand, within rsxB at 1704.806 kb on + strand, within rsxB at 1705.039 kb on + strand at 1705.042 kb on - strand at 1705.235 kb on - strand, within rsxC at 1705.290 kb on + strand, within rsxC at 1705.290 kb on + strand, within rsxC at 1705.308 kb on - strand, within rsxC at 1705.308 kb on - strand, within rsxC at 1705.459 kb on + strand, within rsxC at 1705.459 kb on + strand, within rsxC at 1705.529 kb on - strand, within rsxC at 1705.579 kb on + strand, within rsxC at 1705.727 kb on - strand, within rsxC at 1705.812 kb on + strand, within rsxC at 1705.999 kb on + strand, within rsxC at 1706.363 kb on + strand, within rsxC at 1706.547 kb on - strand, within rsxC at 1706.829 kb on + strand, within rsxC at 1706.859 kb on + strand, within rsxC at 1706.859 kb on + strand, within rsxC at 1706.883 kb on - strand, within rsxC at 1706.883 kb on - strand, within rsxC at 1706.924 kb on + strand, within rsxC at 1707.252 kb on + strand at 1707.252 kb on + strand at 1707.328 kb on + strand, within rsxD at 1707.414 kb on + strand, within rsxD at 1707.414 kb on + strand, within rsxD at 1707.473 kb on - strand, within rsxD at 1707.579 kb on - strand, within rsxD at 1707.696 kb on - strand, within rsxD at 1707.716 kb on + strand, within rsxD at 1707.793 kb on + strand, within rsxD at 1707.803 kb on - strand, within rsxD at 1707.895 kb on + strand, within rsxD at 1707.914 kb on - strand, within rsxD at 1707.924 kb on + strand, within rsxD at 1708.021 kb on + strand, within rsxD at 1708.161 kb on + strand at 1708.161 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0008 mg/ml remove 1,704,048 + rsxA b1627 0.44 +1.4 1,704,090 - rsxA b1627 0.51 +2.3 1,704,235 - rsxA b1627 0.76 +3.2 1,704,376 + +3.2 1,704,376 + +2.7 1,704,377 - +2.3 1,704,525 - rsxB b1628 0.26 +2.9 1,704,772 + rsxB b1628 0.69 +3.1 1,704,806 + rsxB b1628 0.75 +2.0 1,705,039 + +3.3 1,705,042 - +3.2 1,705,235 - rsxC b1629 0.13 +2.4 1,705,290 + rsxC b1629 0.16 +1.9 1,705,290 + rsxC b1629 0.16 +2.4 1,705,308 - rsxC b1629 0.16 +0.6 1,705,308 - rsxC b1629 0.16 +1.6 1,705,459 + rsxC b1629 0.23 +1.5 1,705,459 + rsxC b1629 0.23 +2.9 1,705,529 - rsxC b1629 0.26 +2.2 1,705,579 + rsxC b1629 0.29 +2.9 1,705,727 - rsxC b1629 0.35 +2.4 1,705,812 + rsxC b1629 0.39 +1.7 1,705,999 + rsxC b1629 0.48 +2.4 1,706,363 + rsxC b1629 0.64 +2.2 1,706,547 - rsxC b1629 0.72 +2.2 1,706,829 + rsxC b1629 0.85 +1.3 1,706,859 + rsxC b1629 0.86 +0.6 1,706,859 + rsxC b1629 0.86 +1.0 1,706,883 - rsxC b1629 0.87 +1.4 1,706,883 - rsxC b1629 0.87 +2.8 1,706,924 + rsxC b1629 0.89 +0.3 1,707,252 + +3.9 1,707,252 + +2.4 1,707,328 + rsxD b1630 0.15 +2.6 1,707,414 + rsxD b1630 0.23 +3.2 1,707,414 + rsxD b1630 0.23 +1.7 1,707,473 - rsxD b1630 0.29 +1.9 1,707,579 - rsxD b1630 0.39 +2.6 1,707,696 - rsxD b1630 0.50 +3.2 1,707,716 + rsxD b1630 0.52 +1.9 1,707,793 + rsxD b1630 0.59 +3.0 1,707,803 - rsxD b1630 0.60 +3.0 1,707,895 + rsxD b1630 0.69 -1.4 1,707,914 - rsxD b1630 0.71 +3.2 1,707,924 + rsxD b1630 0.72 +1.7 1,708,021 + rsxD b1630 0.81 +2.2 1,708,161 + +2.3 1,708,161 + +1.7
Or see this region's nucleotide sequence