Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rhlE and ybiA are separated by 231 nucleotides ybiA and dinG are separated by 119 nucleotides dinG and ybiB are separated by 27 nucleotides
b0797: rhlE - RNA helicase (NCBI), at 830,095 to 831,459
rhlE
b0798: ybiA - hypothetical protein (NCBI), at 831,691 to 832,173
ybiA
b0799: dinG - ATP-dependent DNA helicase (NCBI), at 832,293 to 834,443
dinG
b0800: ybiB - hypothetical protein (NCBI), at 834,471 to 835,433
ybiB
Position (kb) 832
833
834
835 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 831.483 kb on + strand at 831.535 kb on - strand at 831.542 kb on - strand at 831.542 kb on - strand at 831.679 kb on + strand at 831.679 kb on + strand at 831.733 kb on - strand at 831.733 kb on - strand at 831.752 kb on + strand, within ybiA at 831.752 kb on + strand, within ybiA at 831.942 kb on - strand, within ybiA at 832.127 kb on + strand at 832.127 kb on + strand at 832.158 kb on - strand at 832.250 kb on + strand at 832.353 kb on - strand at 832.645 kb on + strand, within dinG at 832.645 kb on + strand, within dinG at 832.673 kb on + strand, within dinG at 832.692 kb on + strand, within dinG at 833.081 kb on - strand, within dinG at 833.102 kb on + strand, within dinG at 833.222 kb on + strand, within dinG at 833.222 kb on + strand, within dinG at 833.382 kb on - strand, within dinG at 833.425 kb on + strand, within dinG at 833.425 kb on + strand, within dinG at 833.538 kb on + strand, within dinG at 833.538 kb on + strand, within dinG at 833.644 kb on + strand, within dinG at 833.644 kb on + strand, within dinG at 833.658 kb on + strand, within dinG at 833.658 kb on + strand, within dinG at 833.791 kb on + strand, within dinG at 834.071 kb on - strand, within dinG at 834.071 kb on - strand, within dinG at 834.154 kb on + strand, within dinG at 834.154 kb on + strand, within dinG at 834.347 kb on - strand at 834.347 kb on - strand at 834.349 kb on - strand at 834.349 kb on - strand at 834.535 kb on + strand at 834.535 kb on + strand at 834.550 kb on + strand at 834.559 kb on + strand at 834.559 kb on + strand at 834.564 kb on - strand at 834.564 kb on - strand at 834.642 kb on + strand, within ybiB at 834.642 kb on + strand, within ybiB at 834.734 kb on - strand, within ybiB at 834.799 kb on + strand, within ybiB at 835.009 kb on - strand, within ybiB at 835.090 kb on + strand, within ybiB at 835.163 kb on + strand, within ybiB at 835.179 kb on - strand, within ybiB at 835.207 kb on + strand, within ybiB at 835.207 kb on + strand, within ybiB at 835.239 kb on - strand, within ybiB at 835.239 kb on - strand, within ybiB at 835.294 kb on - strand, within ybiB
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 831,483 + +0.6 831,535 - +0.1 831,542 - -1.0 831,542 - +3.6 831,679 + -1.9 831,679 + -0.1 831,733 - -0.4 831,733 - -1.4 831,752 + ybiA b0798 0.13 -0.6 831,752 + ybiA b0798 0.13 +0.6 831,942 - ybiA b0798 0.52 +0.8 832,127 + +0.8 832,127 + +1.2 832,158 - +0.1 832,250 + +2.2 832,353 - +0.3 832,645 + dinG b0799 0.16 -0.2 832,645 + dinG b0799 0.16 -1.4 832,673 + dinG b0799 0.18 -1.0 832,692 + dinG b0799 0.19 -0.3 833,081 - dinG b0799 0.37 +0.6 833,102 + dinG b0799 0.38 -0.2 833,222 + dinG b0799 0.43 -1.4 833,222 + dinG b0799 0.43 +1.2 833,382 - dinG b0799 0.51 -0.4 833,425 + dinG b0799 0.53 +0.1 833,425 + dinG b0799 0.53 -1.4 833,538 + dinG b0799 0.58 -0.6 833,538 + dinG b0799 0.58 -0.1 833,644 + dinG b0799 0.63 -0.1 833,644 + dinG b0799 0.63 +0.6 833,658 + dinG b0799 0.63 -0.1 833,658 + dinG b0799 0.63 +0.7 833,791 + dinG b0799 0.70 -0.2 834,071 - dinG b0799 0.83 +0.1 834,071 - dinG b0799 0.83 -0.4 834,154 + dinG b0799 0.87 +0.8 834,154 + dinG b0799 0.87 -0.0 834,347 - -2.6 834,347 - -1.2 834,349 - +0.6 834,349 - +0.0 834,535 + +1.5 834,535 + +0.1 834,550 + +1.4 834,559 + +0.4 834,559 + -1.4 834,564 - -0.4 834,564 - +0.3 834,642 + ybiB b0800 0.18 +0.6 834,642 + ybiB b0800 0.18 -0.4 834,734 - ybiB b0800 0.27 -2.4 834,799 + ybiB b0800 0.34 +0.1 835,009 - ybiB b0800 0.56 +0.2 835,090 + ybiB b0800 0.64 -0.2 835,163 + ybiB b0800 0.72 +1.2 835,179 - ybiB b0800 0.74 +0.8 835,207 + ybiB b0800 0.76 -1.4 835,207 + ybiB b0800 0.76 -0.6 835,239 - ybiB b0800 0.80 +0.7 835,239 - ybiB b0800 0.80 +0.8 835,294 - ybiB b0800 0.85 +0.3
Or see this region's nucleotide sequence