Experiment: marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_2400 and Echvi_2401 are separated by 241 nucleotides Echvi_2401 and Echvi_2402 are separated by 72 nucleotides Echvi_2402 and Echvi_2403 overlap by 4 nucleotides
Echvi_2400: Echvi_2400 - CTP synthase, at 2,841,912 to 2,843,534
_2400
Echvi_2401: Echvi_2401 - hypothetical protein, at 2,843,776 to 2,844,039
_2401
Echvi_2402: Echvi_2402 - hypothetical protein, at 2,844,112 to 2,844,579
_2402
Echvi_2403: Echvi_2403 - tRNA nucleotidyltransferase/poly(A) polymerase, at 2,844,576 to 2,846,006
_2403
Position (kb)
2843
2844
2845 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2843.639 kb on - strand at 2843.712 kb on + strand at 2843.758 kb on + strand at 2843.778 kb on - strand at 2843.796 kb on + strand at 2843.824 kb on + strand, within Echvi_2401 at 2843.858 kb on - strand, within Echvi_2401 at 2843.907 kb on + strand, within Echvi_2401 at 2843.910 kb on + strand at 2843.911 kb on - strand, within Echvi_2401 at 2843.911 kb on - strand, within Echvi_2401 at 2844.042 kb on - strand at 2844.064 kb on - strand at 2844.308 kb on + strand, within Echvi_2402 at 2844.308 kb on + strand, within Echvi_2402 at 2844.337 kb on + strand, within Echvi_2402 at 2844.339 kb on + strand, within Echvi_2402 at 2844.339 kb on + strand, within Echvi_2402 at 2844.564 kb on - strand at 2844.564 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction marine broth remove 2,843,639 - +0.5 2,843,712 + -0.2 2,843,758 + -0.0 2,843,778 - +0.2 2,843,796 + -0.6 2,843,824 + Echvi_2401 0.18 +0.3 2,843,858 - Echvi_2401 0.31 +0.6 2,843,907 + Echvi_2401 0.50 +0.8 2,843,910 + +0.8 2,843,911 - Echvi_2401 0.51 -2.5 2,843,911 - Echvi_2401 0.51 +0.8 2,844,042 - -0.5 2,844,064 - -0.3 2,844,308 + Echvi_2402 0.42 -0.5 2,844,308 + Echvi_2402 0.42 +0.2 2,844,337 + Echvi_2402 0.48 +0.7 2,844,339 + Echvi_2402 0.49 +1.2 2,844,339 + Echvi_2402 0.49 +1.4 2,844,564 - +1.9 2,844,564 - -0.5
Or see this region's nucleotide sequence