Experiment: marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0185 and Echvi_0186 are separated by 72 nucleotides Echvi_0186 and Echvi_0187 are separated by 18 nucleotides Echvi_0187 and Echvi_0188 are separated by 382 nucleotides
Echvi_0185: Echvi_0185 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 203,047 to 204,069
_0185
Echvi_0186: Echvi_0186 - Predicted metal-binding, possibly nucleic acid-binding protein, at 204,142 to 204,708
_0186
Echvi_0187: Echvi_0187 - ribosomal protein L32, at 204,727 to 204,918
_0187
Echvi_0188: Echvi_0188 - 3-oxoacyl-(acyl-carrier-protein) synthase III, at 205,301 to 206,299
_0188
Position (kb) 204
205 Strain fitness (log2 ratio) -2
-1
0
1
2 at 204.068 kb on - strand at 204.412 kb on + strand, within Echvi_0186 at 204.512 kb on + strand, within Echvi_0186 at 204.590 kb on + strand, within Echvi_0186 at 204.590 kb on + strand, within Echvi_0186 at 204.590 kb on + strand, within Echvi_0186 at 204.592 kb on + strand, within Echvi_0186 at 205.010 kb on - strand at 205.023 kb on + strand at 205.032 kb on + strand at 205.146 kb on - strand at 205.229 kb on + strand at 205.239 kb on + strand at 205.239 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction marine broth remove 204,068 - -1.0 204,412 + Echvi_0186 0.48 -2.0 204,512 + Echvi_0186 0.65 -0.8 204,590 + Echvi_0186 0.79 +1.8 204,590 + Echvi_0186 0.79 -0.5 204,590 + Echvi_0186 0.79 -0.6 204,592 + Echvi_0186 0.79 +1.2 205,010 - -0.5 205,023 + +0.9 205,032 + -1.4 205,146 - +0.4 205,229 + +0.8 205,239 + -0.7 205,239 + -0.2
Or see this region's nucleotide sequence