Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and thiG overlap by 4 nucleotides thiG and thiS are separated by 1 nucleotides thiS and thiF overlap by 17 nucleotides thiF and thiE overlap by 8 nucleotides
b3990: thiH - thiamine biosynthesis protein ThiH (NCBI), at 4,188,758 to 4,189,891
thiH
b3991: thiG - thiamin biosynthesis, thiazole moiety (VIMSS), at 4,189,888 to 4,190,658
thiG
b4407: thiS - sulfur carrier protein ThiS (NCBI), at 4,190,660 to 4,190,860
thiS
b3992: thiF - thiamin biosynthesis, thiazole moiety (VIMSS), at 4,190,844 to 4,191,599
thiF
b3993: thiE - thiamine-phosphate pyrophosphorylase (NCBI), at 4,191,592 to 4,192,227
thiE
Position (kb)
4190
4191 Strain fitness (log2 ratio)
-2
-1
0
1 at 4189.724 kb on + strand, within thiH at 4189.737 kb on - strand, within thiH at 4189.737 kb on - strand, within thiH at 4189.759 kb on - strand, within thiH at 4189.759 kb on - strand, within thiH at 4190.005 kb on - strand, within thiG at 4190.005 kb on - strand, within thiG at 4190.187 kb on - strand, within thiG at 4190.187 kb on - strand, within thiG at 4190.253 kb on + strand, within thiG at 4190.267 kb on - strand, within thiG at 4190.267 kb on - strand, within thiG at 4190.270 kb on + strand, within thiG at 4190.270 kb on + strand, within thiG at 4190.278 kb on - strand, within thiG at 4190.278 kb on - strand, within thiG at 4190.287 kb on - strand, within thiG at 4190.306 kb on - strand, within thiG at 4190.306 kb on - strand, within thiG at 4190.404 kb on + strand, within thiG at 4190.412 kb on - strand, within thiG at 4190.528 kb on - strand, within thiG at 4190.579 kb on + strand, within thiG at 4190.581 kb on + strand, within thiG at 4190.581 kb on + strand, within thiG at 4190.584 kb on - strand at 4190.586 kb on - strand at 4190.791 kb on + strand, within thiS at 4190.820 kb on + strand, within thiS at 4190.830 kb on + strand, within thiS at 4190.860 kb on - strand at 4190.908 kb on - strand at 4190.968 kb on - strand, within thiF at 4190.968 kb on - strand, within thiF at 4191.030 kb on + strand, within thiF at 4191.121 kb on + strand, within thiF at 4191.121 kb on + strand, within thiF at 4191.129 kb on - strand, within thiF at 4191.234 kb on + strand, within thiF at 4191.236 kb on - strand, within thiF at 4191.248 kb on + strand, within thiF at 4191.269 kb on + strand, within thiF at 4191.282 kb on - strand, within thiF at 4191.310 kb on - strand, within thiF at 4191.368 kb on - strand, within thiF at 4191.376 kb on + strand, within thiF at 4191.468 kb on - strand, within thiF at 4191.475 kb on - strand, within thiF at 4191.475 kb on - strand, within thiF at 4191.483 kb on - strand, within thiF at 4191.483 kb on - strand, within thiF at 4191.489 kb on + strand, within thiF at 4191.489 kb on + strand, within thiF at 4191.489 kb on + strand, within thiF at 4191.489 kb on + strand, within thiF at 4191.680 kb on + strand, within thiE at 4191.690 kb on + strand, within thiE at 4191.731 kb on - strand, within thiE at 4191.755 kb on + strand, within thiE at 4191.755 kb on + strand, within thiE at 4191.859 kb on - strand, within thiE
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 4,189,724 + thiH b3990 0.85 -1.2 4,189,737 - thiH b3990 0.86 -1.8 4,189,737 - thiH b3990 0.86 -1.9 4,189,759 - thiH b3990 0.88 -0.1 4,189,759 - thiH b3990 0.88 -2.4 4,190,005 - thiG b3991 0.15 +0.3 4,190,005 - thiG b3991 0.15 +0.2 4,190,187 - thiG b3991 0.39 +1.2 4,190,187 - thiG b3991 0.39 +1.2 4,190,253 + thiG b3991 0.47 -0.6 4,190,267 - thiG b3991 0.49 -0.6 4,190,267 - thiG b3991 0.49 +1.0 4,190,270 + thiG b3991 0.50 -0.4 4,190,270 + thiG b3991 0.50 +0.6 4,190,278 - thiG b3991 0.51 +0.8 4,190,278 - thiG b3991 0.51 -0.6 4,190,287 - thiG b3991 0.52 -1.5 4,190,306 - thiG b3991 0.54 +0.2 4,190,306 - thiG b3991 0.54 -0.4 4,190,404 + thiG b3991 0.67 -1.8 4,190,412 - thiG b3991 0.68 +0.0 4,190,528 - thiG b3991 0.83 -2.0 4,190,579 + thiG b3991 0.90 +1.2 4,190,581 + thiG b3991 0.90 -0.2 4,190,581 + thiG b3991 0.90 -0.9 4,190,584 - +0.8 4,190,586 - +1.0 4,190,791 + thiS b4407 0.65 -0.2 4,190,820 + thiS b4407 0.80 +0.2 4,190,830 + thiS b4407 0.85 -0.6 4,190,860 - -0.6 4,190,908 - -2.1 4,190,968 - thiF b3992 0.16 +0.5 4,190,968 - thiF b3992 0.16 +1.6 4,191,030 + thiF b3992 0.25 +0.6 4,191,121 + thiF b3992 0.37 +0.6 4,191,121 + thiF b3992 0.37 +0.0 4,191,129 - thiF b3992 0.38 +0.0 4,191,234 + thiF b3992 0.52 +0.1 4,191,236 - thiF b3992 0.52 +0.1 4,191,248 + thiF b3992 0.53 +0.8 4,191,269 + thiF b3992 0.56 -1.6 4,191,282 - thiF b3992 0.58 +0.4 4,191,310 - thiF b3992 0.62 -1.4 4,191,368 - thiF b3992 0.69 -0.2 4,191,376 + thiF b3992 0.70 +0.5 4,191,468 - thiF b3992 0.83 -0.5 4,191,475 - thiF b3992 0.83 -1.3 4,191,475 - thiF b3992 0.83 -0.3 4,191,483 - thiF b3992 0.85 +1.4 4,191,483 - thiF b3992 0.85 -0.7 4,191,489 + thiF b3992 0.85 +0.7 4,191,489 + thiF b3992 0.85 -1.1 4,191,489 + thiF b3992 0.85 +0.2 4,191,489 + thiF b3992 0.85 +0.9 4,191,680 + thiE b3993 0.14 -1.1 4,191,690 + thiE b3993 0.15 -0.5 4,191,731 - thiE b3993 0.22 -0.4 4,191,755 + thiE b3993 0.26 -0.9 4,191,755 + thiE b3993 0.26 -1.5 4,191,859 - thiE b3993 0.42 +0.2
Or see this region's nucleotide sequence