Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjiD and yjiE overlap by 8 nucleotides yjiE and iadA are separated by 64 nucleotides iadA and yjiG are separated by 12 nucleotides yjiG and yjiH overlap by 4 nucleotides
b4326: yjiD - orf, hypothetical protein (VIMSS), at 4,555,016 to 4,555,408
yjiD
b4327: yjiE - predicted DNA-binding transcriptional regulator (NCBI), at 4,555,401 to 4,556,312
yjiE
b4328: iadA - isoaspartyl dipeptidase (NCBI), at 4,556,377 to 4,557,549
iadA
b4329: yjiG - conserved inner membrane protein (NCBI), at 4,557,562 to 4,558,023
yjiG
b4330: yjiH - orf, hypothetical protein (VIMSS), at 4,558,020 to 4,558,703
yjiH
Position (kb)
4556
4557
4558 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4555.398 kb on - strand at 4555.407 kb on - strand at 4555.415 kb on + strand at 4555.415 kb on + strand at 4555.498 kb on + strand at 4555.498 kb on + strand, within yjiE at 4555.667 kb on + strand, within yjiE at 4555.731 kb on + strand, within yjiE at 4555.739 kb on - strand at 4555.764 kb on - strand, within yjiE at 4555.764 kb on - strand, within yjiE at 4555.791 kb on - strand, within yjiE at 4555.976 kb on + strand, within yjiE at 4556.042 kb on - strand, within yjiE at 4556.106 kb on + strand, within yjiE at 4556.168 kb on + strand, within yjiE at 4556.168 kb on + strand, within yjiE at 4556.334 kb on - strand at 4556.382 kb on - strand at 4556.382 kb on - strand at 4556.416 kb on - strand at 4556.449 kb on - strand at 4556.449 kb on - strand at 4556.463 kb on + strand at 4556.526 kb on + strand, within iadA at 4556.552 kb on - strand, within iadA at 4556.556 kb on - strand, within iadA at 4556.568 kb on - strand, within iadA at 4556.596 kb on + strand, within iadA at 4556.596 kb on + strand, within iadA at 4556.602 kb on + strand, within iadA at 4556.644 kb on + strand, within iadA at 4556.908 kb on + strand, within iadA at 4556.942 kb on + strand, within iadA at 4556.953 kb on - strand, within iadA at 4556.953 kb on - strand, within iadA at 4556.955 kb on + strand, within iadA at 4556.982 kb on - strand, within iadA at 4557.054 kb on - strand, within iadA at 4557.054 kb on - strand, within iadA at 4557.073 kb on + strand, within iadA at 4557.120 kb on - strand, within iadA at 4557.138 kb on + strand, within iadA at 4557.246 kb on - strand, within iadA at 4557.249 kb on + strand, within iadA at 4557.257 kb on - strand, within iadA at 4557.509 kb on + strand at 4557.623 kb on - strand, within yjiG at 4557.665 kb on - strand, within yjiG at 4557.665 kb on - strand, within yjiG at 4557.667 kb on - strand, within yjiG at 4557.793 kb on + strand, within yjiG at 4557.793 kb on + strand, within yjiG at 4557.801 kb on - strand, within yjiG at 4557.801 kb on - strand, within yjiG at 4557.837 kb on - strand, within yjiG at 4557.837 kb on - strand, within yjiG at 4558.050 kb on - strand at 4558.104 kb on - strand, within yjiH at 4558.116 kb on + strand, within yjiH at 4558.181 kb on - strand, within yjiH at 4558.185 kb on + strand, within yjiH at 4558.211 kb on - strand, within yjiH at 4558.301 kb on + strand, within yjiH at 4558.328 kb on + strand, within yjiH at 4558.392 kb on + strand, within yjiH at 4558.392 kb on + strand, within yjiH at 4558.428 kb on + strand, within yjiH
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 4,555,398 - +1.1 4,555,407 - -0.5 4,555,415 + +1.1 4,555,415 + -0.1 4,555,498 + +1.1 4,555,498 + yjiE b4327 0.11 -0.9 4,555,667 + yjiE b4327 0.29 -0.7 4,555,731 + yjiE b4327 0.36 +0.2 4,555,739 - -0.9 4,555,764 - yjiE b4327 0.40 -0.1 4,555,764 - yjiE b4327 0.40 +0.4 4,555,791 - yjiE b4327 0.43 -0.2 4,555,976 + yjiE b4327 0.63 +2.5 4,556,042 - yjiE b4327 0.70 -0.3 4,556,106 + yjiE b4327 0.77 +0.5 4,556,168 + yjiE b4327 0.84 -0.7 4,556,168 + yjiE b4327 0.84 -1.8 4,556,334 - +0.5 4,556,382 - +0.8 4,556,382 - -1.1 4,556,416 - +0.6 4,556,449 - -1.3 4,556,449 - -1.3 4,556,463 + -0.4 4,556,526 + iadA b4328 0.13 -2.4 4,556,552 - iadA b4328 0.15 -0.9 4,556,556 - iadA b4328 0.15 -0.5 4,556,568 - iadA b4328 0.16 -0.7 4,556,596 + iadA b4328 0.19 -0.4 4,556,596 + iadA b4328 0.19 +1.4 4,556,602 + iadA b4328 0.19 +0.4 4,556,644 + iadA b4328 0.23 +2.4 4,556,908 + iadA b4328 0.45 -1.3 4,556,942 + iadA b4328 0.48 +0.4 4,556,953 - iadA b4328 0.49 +1.8 4,556,953 - iadA b4328 0.49 -1.5 4,556,955 + iadA b4328 0.49 -1.5 4,556,982 - iadA b4328 0.52 -0.3 4,557,054 - iadA b4328 0.58 +0.1 4,557,054 - iadA b4328 0.58 -1.0 4,557,073 + iadA b4328 0.59 +1.1 4,557,120 - iadA b4328 0.63 -0.3 4,557,138 + iadA b4328 0.65 -0.6 4,557,246 - iadA b4328 0.74 -0.7 4,557,249 + iadA b4328 0.74 -0.5 4,557,257 - iadA b4328 0.75 -0.1 4,557,509 + -0.5 4,557,623 - yjiG b4329 0.13 +1.6 4,557,665 - yjiG b4329 0.22 +1.2 4,557,665 - yjiG b4329 0.22 -1.1 4,557,667 - yjiG b4329 0.23 +1.1 4,557,793 + yjiG b4329 0.50 +0.0 4,557,793 + yjiG b4329 0.50 -0.4 4,557,801 - yjiG b4329 0.52 +0.3 4,557,801 - yjiG b4329 0.52 +0.1 4,557,837 - yjiG b4329 0.60 -0.1 4,557,837 - yjiG b4329 0.60 -0.5 4,558,050 - -0.5 4,558,104 - yjiH b4330 0.12 -0.2 4,558,116 + yjiH b4330 0.14 +0.3 4,558,181 - yjiH b4330 0.24 -0.9 4,558,185 + yjiH b4330 0.24 +0.5 4,558,211 - yjiH b4330 0.28 -0.5 4,558,301 + yjiH b4330 0.41 +1.1 4,558,328 + yjiH b4330 0.45 -2.9 4,558,392 + yjiH b4330 0.54 +0.2 4,558,392 + yjiH b4330 0.54 +1.2 4,558,428 + yjiH b4330 0.60 +0.9
Or see this region's nucleotide sequence