Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrfE and nrfF overlap by 8 nucleotides nrfF and nrfG overlap by 4 nucleotides nrfG and gltP are separated by 341 nucleotides
b4074: nrfE - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE (NCBI), at 4,289,535 to 4,291,193
nrfE
b4075: nrfF - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfF (NCBI), at 4,291,186 to 4,291,569
nrfF
b4076: nrfG - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG (NCBI), at 4,291,566 to 4,292,162
nrfG
b4077: gltP - glutamate/aspartate:proton symporter (NCBI), at 4,292,504 to 4,293,817
gltP
Position (kb)
4291
4292 Strain fitness (log2 ratio)
-2
-1
0
1 at 4290.255 kb on + strand, within nrfE at 4290.255 kb on + strand, within nrfE at 4290.265 kb on - strand, within nrfE at 4290.415 kb on - strand, within nrfE at 4290.415 kb on - strand, within nrfE at 4290.441 kb on - strand, within nrfE at 4290.443 kb on + strand, within nrfE at 4290.443 kb on + strand, within nrfE at 4290.465 kb on + strand, within nrfE at 4290.465 kb on - strand, within nrfE at 4290.465 kb on - strand, within nrfE at 4290.566 kb on + strand, within nrfE at 4290.572 kb on - strand, within nrfE at 4290.619 kb on + strand, within nrfE at 4290.619 kb on + strand, within nrfE at 4290.726 kb on + strand, within nrfE at 4290.797 kb on - strand, within nrfE at 4290.832 kb on + strand, within nrfE at 4290.839 kb on + strand, within nrfE at 4290.839 kb on + strand, within nrfE at 4290.847 kb on - strand, within nrfE at 4290.930 kb on + strand, within nrfE at 4290.952 kb on + strand, within nrfE at 4291.075 kb on + strand at 4291.075 kb on + strand at 4291.155 kb on + strand at 4291.208 kb on - strand at 4291.236 kb on + strand, within nrfF at 4291.270 kb on + strand, within nrfF at 4291.423 kb on - strand, within nrfF at 4291.464 kb on + strand, within nrfF at 4291.472 kb on - strand, within nrfF at 4291.483 kb on + strand, within nrfF at 4291.497 kb on + strand, within nrfF at 4291.503 kb on + strand, within nrfF at 4291.503 kb on + strand, within nrfF at 4291.711 kb on - strand, within nrfG at 4291.800 kb on - strand, within nrfG at 4291.854 kb on + strand, within nrfG at 4291.859 kb on + strand, within nrfG at 4291.911 kb on + strand, within nrfG at 4291.911 kb on + strand, within nrfG at 4291.934 kb on + strand, within nrfG at 4291.982 kb on + strand, within nrfG at 4291.982 kb on + strand, within nrfG at 4292.106 kb on + strand at 4292.109 kb on + strand at 4292.112 kb on - strand at 4292.112 kb on - strand at 4292.114 kb on + strand at 4292.114 kb on + strand at 4292.131 kb on - strand at 4292.368 kb on + strand at 4292.368 kb on + strand at 4292.388 kb on + strand at 4292.479 kb on + strand at 4292.479 kb on + strand at 4292.479 kb on + strand at 4292.479 kb on + strand at 4292.479 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 4,290,255 + nrfE b4074 0.43 -0.4 4,290,255 + nrfE b4074 0.43 -0.2 4,290,265 - nrfE b4074 0.44 +0.5 4,290,415 - nrfE b4074 0.53 -0.5 4,290,415 - nrfE b4074 0.53 +0.5 4,290,441 - nrfE b4074 0.55 -0.1 4,290,443 + nrfE b4074 0.55 +0.3 4,290,443 + nrfE b4074 0.55 +0.5 4,290,465 + nrfE b4074 0.56 +0.8 4,290,465 - nrfE b4074 0.56 +0.8 4,290,465 - nrfE b4074 0.56 -1.4 4,290,566 + nrfE b4074 0.62 +1.3 4,290,572 - nrfE b4074 0.63 +0.2 4,290,619 + nrfE b4074 0.65 -0.2 4,290,619 + nrfE b4074 0.65 -0.3 4,290,726 + nrfE b4074 0.72 -0.0 4,290,797 - nrfE b4074 0.76 -0.0 4,290,832 + nrfE b4074 0.78 +0.3 4,290,839 + nrfE b4074 0.79 -0.8 4,290,839 + nrfE b4074 0.79 +0.6 4,290,847 - nrfE b4074 0.79 +0.0 4,290,930 + nrfE b4074 0.84 +1.4 4,290,952 + nrfE b4074 0.85 -0.2 4,291,075 + +0.4 4,291,075 + -0.8 4,291,155 + +1.0 4,291,208 - +0.4 4,291,236 + nrfF b4075 0.13 +0.7 4,291,270 + nrfF b4075 0.22 -1.6 4,291,423 - nrfF b4075 0.62 +0.2 4,291,464 + nrfF b4075 0.72 -1.3 4,291,472 - nrfF b4075 0.74 -1.2 4,291,483 + nrfF b4075 0.77 +1.4 4,291,497 + nrfF b4075 0.81 -1.1 4,291,503 + nrfF b4075 0.83 -0.2 4,291,503 + nrfF b4075 0.83 -0.8 4,291,711 - nrfG b4076 0.24 -2.7 4,291,800 - nrfG b4076 0.39 +0.9 4,291,854 + nrfG b4076 0.48 -1.0 4,291,859 + nrfG b4076 0.49 +0.1 4,291,911 + nrfG b4076 0.58 +0.2 4,291,911 + nrfG b4076 0.58 -1.6 4,291,934 + nrfG b4076 0.62 -1.9 4,291,982 + nrfG b4076 0.70 -0.5 4,291,982 + nrfG b4076 0.70 -0.8 4,292,106 + -0.8 4,292,109 + +0.8 4,292,112 - -0.8 4,292,112 - +0.7 4,292,114 + +0.6 4,292,114 + -0.4 4,292,131 - +0.8 4,292,368 + +0.2 4,292,368 + +0.6 4,292,388 + +0.4 4,292,479 + -0.3 4,292,479 + -0.5 4,292,479 + -1.1 4,292,479 + +0.2 4,292,479 + +0.2
Or see this region's nucleotide sequence