Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhbJ and npr overlap by 4 nucleotides npr and yrbL are separated by 213 nucleotides yrbL and mtgA overlap by 4 nucleotides mtgA and yhbL overlap by 4 nucleotides
b3205: yhbJ - hypothetical protein (NCBI), at 3,345,137 to 3,345,991
yhbJ
b3206: npr - phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI), at 3,345,988 to 3,346,260
npr
b3207: yrbL - hypothetical protein (NCBI), at 3,346,474 to 3,347,106
yrbL
b3208: mtgA - monofunctional biosynthetic peptidoglycan transglycosylase (NCBI), at 3,347,103 to 3,347,831
mtgA
b3209: yhbL - sigma cross-reacting protein 27A (SCRP-27A) (VIMSS), at 3,347,828 to 3,348,481
yhbL
Position (kb)
3346
3347
3348 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3345.698 kb on + strand, within yhbJ at 3345.698 kb on + strand, within yhbJ at 3345.735 kb on + strand, within yhbJ at 3345.863 kb on - strand, within yhbJ at 3345.863 kb on - strand, within yhbJ at 3345.892 kb on - strand, within yhbJ at 3345.892 kb on - strand, within yhbJ at 3345.909 kb on - strand at 3345.953 kb on + strand at 3346.034 kb on - strand, within npr at 3346.147 kb on + strand, within npr at 3346.173 kb on - strand, within npr at 3346.174 kb on + strand, within npr at 3346.174 kb on + strand, within npr at 3346.186 kb on - strand, within npr at 3346.196 kb on - strand, within npr at 3346.286 kb on + strand at 3346.286 kb on + strand at 3346.444 kb on - strand at 3346.487 kb on - strand at 3346.655 kb on + strand, within yrbL at 3346.688 kb on - strand, within yrbL at 3346.697 kb on - strand, within yrbL at 3346.795 kb on + strand, within yrbL at 3346.809 kb on + strand, within yrbL at 3346.921 kb on + strand, within yrbL at 3346.921 kb on + strand, within yrbL at 3346.932 kb on - strand, within yrbL at 3346.965 kb on - strand, within yrbL at 3346.972 kb on + strand, within yrbL at 3346.980 kb on - strand, within yrbL at 3346.980 kb on - strand, within yrbL at 3346.986 kb on + strand, within yrbL at 3346.986 kb on + strand, within yrbL at 3347.046 kb on + strand at 3347.086 kb on - strand at 3347.092 kb on - strand at 3347.111 kb on + strand at 3347.151 kb on - strand at 3347.178 kb on + strand, within mtgA at 3347.178 kb on + strand, within mtgA at 3347.178 kb on + strand, within mtgA at 3347.178 kb on + strand, within mtgA at 3347.184 kb on + strand, within mtgA at 3347.192 kb on - strand, within mtgA at 3347.205 kb on - strand, within mtgA at 3347.205 kb on - strand, within mtgA at 3347.226 kb on + strand, within mtgA at 3347.226 kb on + strand, within mtgA at 3347.278 kb on + strand, within mtgA at 3347.278 kb on + strand, within mtgA at 3347.311 kb on + strand, within mtgA at 3347.311 kb on + strand, within mtgA at 3347.386 kb on + strand at 3347.400 kb on + strand, within mtgA at 3347.430 kb on + strand, within mtgA at 3347.466 kb on + strand, within mtgA at 3347.515 kb on + strand, within mtgA at 3347.520 kb on + strand, within mtgA at 3347.528 kb on - strand, within mtgA at 3347.528 kb on - strand, within mtgA at 3347.534 kb on - strand, within mtgA at 3347.621 kb on + strand, within mtgA at 3347.634 kb on - strand, within mtgA at 3347.634 kb on - strand, within mtgA at 3347.710 kb on - strand, within mtgA at 3347.713 kb on + strand, within mtgA at 3347.725 kb on + strand, within mtgA at 3347.725 kb on + strand, within mtgA at 3347.738 kb on - strand, within mtgA at 3347.778 kb on - strand at 3347.820 kb on - strand at 3347.904 kb on + strand, within yhbL at 3347.904 kb on + strand, within yhbL at 3347.911 kb on - strand, within yhbL at 3347.916 kb on - strand, within yhbL at 3347.975 kb on - strand, within yhbL at 3348.075 kb on + strand, within yhbL
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 3,345,698 + yhbJ b3205 0.66 +0.5 3,345,698 + yhbJ b3205 0.66 +0.0 3,345,735 + yhbJ b3205 0.70 -1.1 3,345,863 - yhbJ b3205 0.85 -0.1 3,345,863 - yhbJ b3205 0.85 +1.4 3,345,892 - yhbJ b3205 0.88 +1.3 3,345,892 - yhbJ b3205 0.88 -0.4 3,345,909 - -1.3 3,345,953 + +3.0 3,346,034 - npr b3206 0.17 +0.5 3,346,147 + npr b3206 0.58 -0.3 3,346,173 - npr b3206 0.68 +1.0 3,346,174 + npr b3206 0.68 -2.4 3,346,174 + npr b3206 0.68 +0.3 3,346,186 - npr b3206 0.73 -0.3 3,346,196 - npr b3206 0.76 +0.2 3,346,286 + -0.8 3,346,286 + -1.0 3,346,444 - -0.8 3,346,487 - +0.2 3,346,655 + yrbL b3207 0.29 -0.6 3,346,688 - yrbL b3207 0.34 -0.2 3,346,697 - yrbL b3207 0.35 -0.1 3,346,795 + yrbL b3207 0.51 -0.4 3,346,809 + yrbL b3207 0.53 +0.7 3,346,921 + yrbL b3207 0.71 -0.2 3,346,921 + yrbL b3207 0.71 +0.0 3,346,932 - yrbL b3207 0.72 -0.6 3,346,965 - yrbL b3207 0.78 -0.5 3,346,972 + yrbL b3207 0.79 -2.4 3,346,980 - yrbL b3207 0.80 -1.0 3,346,980 - yrbL b3207 0.80 -2.3 3,346,986 + yrbL b3207 0.81 -1.2 3,346,986 + yrbL b3207 0.81 +0.0 3,347,046 + -2.1 3,347,086 - -0.1 3,347,092 - +0.0 3,347,111 + -1.3 3,347,151 - -0.1 3,347,178 + mtgA b3208 0.10 +0.0 3,347,178 + mtgA b3208 0.10 +0.7 3,347,178 + mtgA b3208 0.10 +1.0 3,347,178 + mtgA b3208 0.10 +3.0 3,347,184 + mtgA b3208 0.11 +0.6 3,347,192 - mtgA b3208 0.12 +0.4 3,347,205 - mtgA b3208 0.14 +0.0 3,347,205 - mtgA b3208 0.14 -1.3 3,347,226 + mtgA b3208 0.17 +1.0 3,347,226 + mtgA b3208 0.17 +1.0 3,347,278 + mtgA b3208 0.24 -1.4 3,347,278 + mtgA b3208 0.24 +1.0 3,347,311 + mtgA b3208 0.29 -0.4 3,347,311 + mtgA b3208 0.29 -2.0 3,347,386 + +1.0 3,347,400 + mtgA b3208 0.41 +0.4 3,347,430 + mtgA b3208 0.45 +0.9 3,347,466 + mtgA b3208 0.50 +0.3 3,347,515 + mtgA b3208 0.57 -1.4 3,347,520 + mtgA b3208 0.57 +0.9 3,347,528 - mtgA b3208 0.58 +1.0 3,347,528 - mtgA b3208 0.58 +0.5 3,347,534 - mtgA b3208 0.59 +0.3 3,347,621 + mtgA b3208 0.71 +0.9 3,347,634 - mtgA b3208 0.73 -2.0 3,347,634 - mtgA b3208 0.73 -0.3 3,347,710 - mtgA b3208 0.83 -1.0 3,347,713 + mtgA b3208 0.84 -0.8 3,347,725 + mtgA b3208 0.85 -0.2 3,347,725 + mtgA b3208 0.85 +0.1 3,347,738 - mtgA b3208 0.87 +1.0 3,347,778 - -0.4 3,347,820 - -0.1 3,347,904 + yhbL b3209 0.12 +1.8 3,347,904 + yhbL b3209 0.12 +3.8 3,347,911 - yhbL b3209 0.13 +0.9 3,347,916 - yhbL b3209 0.13 +0.3 3,347,975 - yhbL b3209 0.22 -1.8 3,348,075 + yhbL b3209 0.38 -3.0
Or see this region's nucleotide sequence