Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glcD and glcC are separated by 250 nucleotides glcC and yghO are separated by 6 nucleotides
b2979: glcD - glycolate dehydrogenase, putative FAD-linked subunit (from data) , at 3,124,544 to 3,126,043
glcD
b2980: glcC - DNA-binding transcriptional dual regulator, glycolate-binding (NCBI), at 3,126,294 to 3,127,058
glcC
b2981: yghO - predicted DNA-binding transcriptional regulator (RefSeq), at 3,127,065 to 3,128,165
yghO
Position (kb)
3126
3127
3128 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3125.297 kb on - strand, within glcD at 3125.297 kb on - strand, within glcD at 3125.518 kb on - strand, within glcD at 3125.518 kb on - strand, within glcD at 3125.608 kb on + strand, within glcD at 3125.610 kb on - strand, within glcD at 3125.663 kb on + strand, within glcD at 3125.663 kb on + strand, within glcD at 3125.707 kb on - strand, within glcD at 3125.961 kb on + strand at 3125.962 kb on + strand at 3125.969 kb on - strand at 3125.979 kb on - strand at 3125.979 kb on - strand at 3125.983 kb on - strand at 3126.027 kb on + strand at 3126.066 kb on - strand at 3126.280 kb on - strand at 3126.282 kb on - strand at 3126.282 kb on - strand at 3126.345 kb on - strand at 3126.396 kb on - strand, within glcC at 3126.427 kb on + strand, within glcC at 3126.510 kb on + strand, within glcC at 3126.598 kb on + strand, within glcC at 3126.626 kb on + strand, within glcC at 3126.740 kb on - strand, within glcC at 3126.753 kb on - strand, within glcC at 3126.865 kb on - strand, within glcC at 3126.889 kb on + strand, within glcC at 3126.926 kb on - strand, within glcC at 3127.086 kb on - strand at 3127.101 kb on - strand at 3127.148 kb on - strand at 3127.169 kb on - strand at 3127.169 kb on - strand at 3127.234 kb on - strand, within yghO at 3127.274 kb on - strand, within yghO at 3127.274 kb on - strand, within yghO at 3127.435 kb on + strand, within yghO at 3127.566 kb on + strand, within yghO at 3127.566 kb on + strand, within yghO at 3127.607 kb on + strand, within yghO at 3127.697 kb on - strand, within yghO at 3127.762 kb on - strand, within yghO at 3127.762 kb on - strand, within yghO at 3127.762 kb on - strand, within yghO at 3127.764 kb on + strand, within yghO at 3127.772 kb on - strand, within yghO at 3127.846 kb on - strand, within yghO at 3127.909 kb on - strand, within yghO at 3127.909 kb on - strand, within yghO at 3127.966 kb on + strand, within yghO at 3128.034 kb on + strand, within yghO at 3128.034 kb on + strand, within yghO at 3128.034 kb on + strand, within yghO
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 3,125,297 - glcD b2979 0.50 +0.8 3,125,297 - glcD b2979 0.50 -1.0 3,125,518 - glcD b2979 0.65 +0.2 3,125,518 - glcD b2979 0.65 +0.4 3,125,608 + glcD b2979 0.71 -1.2 3,125,610 - glcD b2979 0.71 -0.1 3,125,663 + glcD b2979 0.75 +1.3 3,125,663 + glcD b2979 0.75 -1.6 3,125,707 - glcD b2979 0.78 +0.6 3,125,961 + -0.6 3,125,962 + +0.2 3,125,969 - -1.3 3,125,979 - +0.3 3,125,979 - +1.0 3,125,983 - -1.2 3,126,027 + +1.2 3,126,066 - +0.2 3,126,280 - -0.4 3,126,282 - +0.2 3,126,282 - +0.1 3,126,345 - -0.0 3,126,396 - glcC b2980 0.13 -1.1 3,126,427 + glcC b2980 0.17 -0.2 3,126,510 + glcC b2980 0.28 -0.1 3,126,598 + glcC b2980 0.40 -2.8 3,126,626 + glcC b2980 0.43 -2.3 3,126,740 - glcC b2980 0.58 +0.3 3,126,753 - glcC b2980 0.60 -1.1 3,126,865 - glcC b2980 0.75 -1.0 3,126,889 + glcC b2980 0.78 -1.7 3,126,926 - glcC b2980 0.83 -0.3 3,127,086 - +1.3 3,127,101 - -0.2 3,127,148 - -0.1 3,127,169 - +0.5 3,127,169 - -1.1 3,127,234 - yghO b2981 0.15 -0.1 3,127,274 - yghO b2981 0.19 -0.4 3,127,274 - yghO b2981 0.19 -0.0 3,127,435 + yghO b2981 0.34 +0.3 3,127,566 + yghO b2981 0.46 +0.7 3,127,566 + yghO b2981 0.46 +0.5 3,127,607 + yghO b2981 0.49 -0.9 3,127,697 - yghO b2981 0.57 -0.1 3,127,762 - yghO b2981 0.63 -0.1 3,127,762 - yghO b2981 0.63 +0.8 3,127,762 - yghO b2981 0.63 -1.0 3,127,764 + yghO b2981 0.63 -0.8 3,127,772 - yghO b2981 0.64 -0.3 3,127,846 - yghO b2981 0.71 +1.3 3,127,909 - yghO b2981 0.77 -2.5 3,127,909 - yghO b2981 0.77 +0.4 3,127,966 + yghO b2981 0.82 -0.0 3,128,034 + yghO b2981 0.88 -0.7 3,128,034 + yghO b2981 0.88 -1.0 3,128,034 + yghO b2981 0.88 +0.2
Or see this region's nucleotide sequence