Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hcaE and hcaF overlap by 4 nucleotides hcaF and hcaC overlap by 1 nucleotides hcaC and hcaB overlap by 4 nucleotides hcaB and hcaD are separated by 9 nucleotides
b2538: hcaE - 3-phenylpropionate dioxygenase, large (alpha) subunit (NCBI), at 2,667,054 to 2,668,415
hcaE
b2539: hcaF - 3-phenylpropionate dioxygenase, small (beta) subunit (NCBI), at 2,668,412 to 2,668,930
hcaF
b2540: hcaC - 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI), at 2,668,930 to 2,669,250
hcaC
b2541: hcaB - 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase (NCBI), at 2,669,247 to 2,670,059
hcaB
b2542: hcaD - phenylpropionate dioxygenase, ferredoxin reductase subunit (NCBI), at 2,670,069 to 2,671,271
hcaD
Position (kb)
2669
2670
2671 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2668.253 kb on + strand, within hcaE at 2668.253 kb on + strand, within hcaE at 2668.363 kb on + strand at 2668.539 kb on - strand, within hcaF at 2668.551 kb on - strand, within hcaF at 2668.551 kb on - strand, within hcaF at 2668.676 kb on + strand, within hcaF at 2668.676 kb on + strand, within hcaF at 2668.709 kb on + strand, within hcaF at 2668.715 kb on + strand, within hcaF at 2668.736 kb on - strand, within hcaF at 2668.759 kb on + strand, within hcaF at 2668.888 kb on - strand at 2668.888 kb on - strand at 2668.925 kb on - strand at 2668.932 kb on - strand at 2668.979 kb on - strand, within hcaC at 2669.083 kb on + strand, within hcaC at 2669.112 kb on - strand, within hcaC at 2669.166 kb on + strand, within hcaC at 2669.295 kb on + strand at 2669.442 kb on - strand, within hcaB at 2669.469 kb on - strand at 2669.662 kb on + strand, within hcaB at 2669.678 kb on - strand, within hcaB at 2669.678 kb on - strand, within hcaB at 2669.716 kb on - strand, within hcaB at 2669.716 kb on - strand, within hcaB at 2669.827 kb on + strand, within hcaB at 2669.827 kb on + strand, within hcaB at 2669.835 kb on - strand, within hcaB at 2669.835 kb on - strand, within hcaB at 2669.946 kb on + strand, within hcaB at 2669.947 kb on - strand, within hcaB at 2669.951 kb on + strand, within hcaB at 2669.951 kb on + strand, within hcaB at 2670.153 kb on + strand at 2670.199 kb on + strand, within hcaD at 2670.427 kb on - strand, within hcaD at 2670.427 kb on - strand, within hcaD at 2670.447 kb on + strand, within hcaD at 2670.450 kb on - strand, within hcaD at 2670.450 kb on - strand, within hcaD at 2670.576 kb on - strand, within hcaD at 2670.576 kb on - strand, within hcaD at 2670.576 kb on - strand, within hcaD at 2670.603 kb on + strand, within hcaD at 2670.836 kb on - strand, within hcaD at 2670.927 kb on - strand, within hcaD at 2670.994 kb on - strand, within hcaD at 2670.994 kb on - strand, within hcaD at 2671.037 kb on + strand, within hcaD
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 2,668,253 + hcaE b2538 0.88 -1.9 2,668,253 + hcaE b2538 0.88 +0.9 2,668,363 + +0.6 2,668,539 - hcaF b2539 0.24 -1.3 2,668,551 - hcaF b2539 0.27 +0.9 2,668,551 - hcaF b2539 0.27 -0.1 2,668,676 + hcaF b2539 0.51 +1.3 2,668,676 + hcaF b2539 0.51 +1.2 2,668,709 + hcaF b2539 0.57 -0.7 2,668,715 + hcaF b2539 0.58 +1.1 2,668,736 - hcaF b2539 0.62 -0.0 2,668,759 + hcaF b2539 0.67 +0.7 2,668,888 - -0.7 2,668,888 - -0.7 2,668,925 - -1.8 2,668,932 - +0.1 2,668,979 - hcaC b2540 0.15 -0.7 2,669,083 + hcaC b2540 0.48 +0.3 2,669,112 - hcaC b2540 0.57 +1.2 2,669,166 + hcaC b2540 0.74 -1.1 2,669,295 + -0.5 2,669,442 - hcaB b2541 0.24 -0.5 2,669,469 - -0.1 2,669,662 + hcaB b2541 0.51 +0.8 2,669,678 - hcaB b2541 0.53 +0.9 2,669,678 - hcaB b2541 0.53 +0.7 2,669,716 - hcaB b2541 0.58 +0.7 2,669,716 - hcaB b2541 0.58 +0.9 2,669,827 + hcaB b2541 0.71 +1.8 2,669,827 + hcaB b2541 0.71 -0.2 2,669,835 - hcaB b2541 0.72 +0.5 2,669,835 - hcaB b2541 0.72 +1.9 2,669,946 + hcaB b2541 0.86 -0.1 2,669,947 - hcaB b2541 0.86 -1.3 2,669,951 + hcaB b2541 0.87 +2.1 2,669,951 + hcaB b2541 0.87 +0.9 2,670,153 + -0.9 2,670,199 + hcaD b2542 0.11 +1.2 2,670,427 - hcaD b2542 0.30 -0.2 2,670,427 - hcaD b2542 0.30 -0.1 2,670,447 + hcaD b2542 0.31 -0.7 2,670,450 - hcaD b2542 0.32 -1.1 2,670,450 - hcaD b2542 0.32 +0.5 2,670,576 - hcaD b2542 0.42 +1.8 2,670,576 - hcaD b2542 0.42 +0.5 2,670,576 - hcaD b2542 0.42 -0.7 2,670,603 + hcaD b2542 0.44 -0.9 2,670,836 - hcaD b2542 0.64 +0.6 2,670,927 - hcaD b2542 0.71 +0.8 2,670,994 - hcaD b2542 0.77 -0.7 2,670,994 - hcaD b2542 0.77 +2.0 2,671,037 + hcaD b2542 0.80 +0.5
Or see this region's nucleotide sequence