Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yojI and alkB are separated by 75 nucleotides alkB and ada overlap by 1 nucleotides ada and yojL are separated by 73 nucleotides
b2211: yojI - fused predicted multidrug transport subunits of ABC superfamily: membrane component/ATP-binding component (NCBI), at 2,304,994 to 2,306,637
yojI
b2212: alkB - oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI), at 2,306,713 to 2,307,363
alkB
b2213: ada - fused DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase (NCBI), at 2,307,363 to 2,308,427
ada
b2214: yojL - predicted thiamine biosynthesis lipoprotein (NCBI), at 2,308,501 to 2,309,556
yojL
Position (kb)
2307
2308
2309 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2306.433 kb on - strand, within yojI at 2306.444 kb on - strand, within yojI at 2306.444 kb on - strand, within yojI at 2306.444 kb on - strand, within yojI at 2306.500 kb on + strand at 2306.572 kb on + strand at 2306.572 kb on + strand at 2306.783 kb on + strand, within alkB at 2306.801 kb on - strand, within alkB at 2306.942 kb on - strand, within alkB at 2306.942 kb on - strand, within alkB at 2307.059 kb on + strand, within alkB at 2307.168 kb on - strand, within alkB at 2307.181 kb on - strand, within alkB at 2307.336 kb on + strand at 2307.336 kb on + strand at 2307.336 kb on + strand at 2307.368 kb on - strand at 2307.393 kb on + strand at 2307.549 kb on - strand, within ada at 2307.562 kb on - strand, within ada at 2307.583 kb on + strand, within ada at 2307.593 kb on + strand, within ada at 2307.849 kb on - strand, within ada at 2308.153 kb on + strand, within ada at 2308.153 kb on + strand, within ada at 2308.162 kb on + strand, within ada at 2308.327 kb on + strand at 2308.337 kb on - strand at 2308.651 kb on + strand, within yojL at 2308.661 kb on - strand at 2308.661 kb on - strand, within yojL at 2308.745 kb on - strand, within yojL at 2308.784 kb on - strand, within yojL at 2308.796 kb on - strand, within yojL at 2308.916 kb on + strand, within yojL at 2308.916 kb on + strand, within yojL at 2308.964 kb on - strand, within yojL at 2308.964 kb on - strand, within yojL at 2309.070 kb on - strand, within yojL at 2309.100 kb on + strand, within yojL at 2309.123 kb on - strand, within yojL at 2309.286 kb on - strand, within yojL at 2309.286 kb on - strand, within yojL
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 2,306,433 - yojI b2211 0.88 -2.7 2,306,444 - yojI b2211 0.88 -1.5 2,306,444 - yojI b2211 0.88 +2.0 2,306,444 - yojI b2211 0.88 -1.2 2,306,500 + +1.3 2,306,572 + +0.5 2,306,572 + -2.3 2,306,783 + alkB b2212 0.11 +0.6 2,306,801 - alkB b2212 0.14 -1.4 2,306,942 - alkB b2212 0.35 +1.7 2,306,942 - alkB b2212 0.35 +0.4 2,307,059 + alkB b2212 0.53 -0.1 2,307,168 - alkB b2212 0.70 -1.8 2,307,181 - alkB b2212 0.72 +1.1 2,307,336 + +1.2 2,307,336 + +0.9 2,307,336 + -0.7 2,307,368 - +0.9 2,307,393 + +0.2 2,307,549 - ada b2213 0.17 -2.1 2,307,562 - ada b2213 0.19 +0.9 2,307,583 + ada b2213 0.21 +1.3 2,307,593 + ada b2213 0.22 -1.9 2,307,849 - ada b2213 0.46 -0.1 2,308,153 + ada b2213 0.74 -2.8 2,308,153 + ada b2213 0.74 -2.1 2,308,162 + ada b2213 0.75 +1.9 2,308,327 + +1.9 2,308,337 - +0.3 2,308,651 + yojL b2214 0.14 -0.7 2,308,661 - -0.1 2,308,661 - yojL b2214 0.15 -1.1 2,308,745 - yojL b2214 0.23 -0.9 2,308,784 - yojL b2214 0.27 -0.4 2,308,796 - yojL b2214 0.28 +0.9 2,308,916 + yojL b2214 0.39 +0.3 2,308,916 + yojL b2214 0.39 +0.4 2,308,964 - yojL b2214 0.44 -0.8 2,308,964 - yojL b2214 0.44 +0.2 2,309,070 - yojL b2214 0.54 +1.9 2,309,100 + yojL b2214 0.57 -0.5 2,309,123 - yojL b2214 0.59 +0.2 2,309,286 - yojL b2214 0.74 -1.1 2,309,286 - yojL b2214 0.74 +1.9
Or see this region's nucleotide sequence