Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeeZ and yoeB are separated by 82 nucleotides yoeB and yefM overlap by 4 nucleotides yefM and hisL are separated by 282 nucleotides hisL and hisG are separated by 145 nucleotides
b2016: yeeZ - predicted epimerase, with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,086,328 to 2,087,152
yeeZ
b4539: yoeB - toxin of the YoeB-YefM toxin-antitoxin system (NCBI), at 2,087,235 to 2,087,489
yoeB
b2017: yefM - orf, hypothetical protein (VIMSS), at 2,087,486 to 2,087,737
yefM
b2018: hisL - his operon leader peptide (NCBI), at 2,088,020 to 2,088,070
hisL
b2019: hisG - ATP phosphoribosyltransferase (NCBI), at 2,088,216 to 2,089,115
hisG
Position (kb)
2087
2088 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2086.500 kb on + strand, within yeeZ at 2086.556 kb on - strand, within yeeZ at 2086.579 kb on + strand, within yeeZ at 2086.660 kb on + strand, within yeeZ at 2086.660 kb on + strand, within yeeZ at 2086.669 kb on + strand, within yeeZ at 2086.672 kb on - strand, within yeeZ at 2086.672 kb on - strand, within yeeZ at 2086.871 kb on - strand, within yeeZ at 2086.947 kb on - strand, within yeeZ at 2087.058 kb on + strand, within yeeZ at 2087.122 kb on + strand at 2087.178 kb on - strand at 2087.178 kb on - strand at 2087.187 kb on - strand at 2087.197 kb on - strand at 2087.364 kb on - strand, within yoeB at 2087.506 kb on - strand at 2087.506 kb on - strand at 2087.544 kb on + strand, within yefM at 2087.642 kb on - strand, within yefM at 2087.867 kb on + strand at 2088.113 kb on - strand at 2088.203 kb on - strand at 2088.203 kb on - strand at 2088.316 kb on + strand, within hisG at 2088.484 kb on - strand, within hisG at 2088.602 kb on + strand, within hisG at 2088.610 kb on - strand, within hisG at 2088.610 kb on - strand, within hisG at 2088.657 kb on - strand, within hisG at 2088.681 kb on - strand, within hisG
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 2,086,500 + yeeZ b2016 0.21 -0.1 2,086,556 - yeeZ b2016 0.28 +0.6 2,086,579 + yeeZ b2016 0.30 -1.8 2,086,660 + yeeZ b2016 0.40 +0.0 2,086,660 + yeeZ b2016 0.40 -0.1 2,086,669 + yeeZ b2016 0.41 -1.4 2,086,672 - yeeZ b2016 0.42 +0.5 2,086,672 - yeeZ b2016 0.42 +2.0 2,086,871 - yeeZ b2016 0.66 +0.2 2,086,947 - yeeZ b2016 0.75 +0.8 2,087,058 + yeeZ b2016 0.88 -0.2 2,087,122 + -1.1 2,087,178 - +0.7 2,087,178 - -0.6 2,087,187 - -0.1 2,087,197 - +2.5 2,087,364 - yoeB b4539 0.51 -0.7 2,087,506 - -0.3 2,087,506 - -0.1 2,087,544 + yefM b2017 0.23 -0.1 2,087,642 - yefM b2017 0.62 -0.8 2,087,867 + -1.2 2,088,113 - +0.7 2,088,203 - -0.0 2,088,203 - +0.9 2,088,316 + hisG b2019 0.11 +0.1 2,088,484 - hisG b2019 0.30 +1.1 2,088,602 + hisG b2019 0.43 -2.1 2,088,610 - hisG b2019 0.44 +0.0 2,088,610 - hisG b2019 0.44 +0.2 2,088,657 - hisG b2019 0.49 -1.9 2,088,681 - hisG b2019 0.52 +1.3
Or see this region's nucleotide sequence