Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yobD and b1821 are separated by 428 nucleotides b1821 and rrmA overlap by 4 nucleotides rrmA and cspC are separated by 165 nucleotides cspC and yobF are separated by 12 nucleotides
b1820: yobD - hypothetical protein (NCBI), at 1,902,825 to 1,903,283
yobD
b1821: b1821 - orf, hypothetical protein (VIMSS), at 1,903,712 to 1,904,278
b1821
b1822: rrmA - 23S rRNA m1G745 methyltransferase (NCBI), at 1,904,275 to 1,905,084
rrmA
b1823: cspC - stress protein, member of the CspA-family (NCBI), at 1,905,250 to 1,905,459
cspC
b1824: yobF - hypothetical protein (NCBI), at 1,905,472 to 1,905,615
yobF
Position (kb)
1904
1905
1906 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1903.496 kb on - strand at 1903.498 kb on - strand at 1903.529 kb on + strand at 1903.537 kb on - strand at 1903.548 kb on + strand at 1903.553 kb on + strand at 1903.558 kb on + strand at 1903.558 kb on + strand at 1903.583 kb on + strand at 1903.602 kb on - strand at 1903.602 kb on - strand at 1903.639 kb on - strand at 1903.654 kb on - strand at 1903.702 kb on + strand at 1903.777 kb on - strand, within b1821 at 1903.777 kb on - strand, within b1821 at 1904.065 kb on - strand, within b1821 at 1904.087 kb on - strand, within b1821 at 1904.603 kb on + strand, within rrmA at 1904.603 kb on + strand, within rrmA at 1904.691 kb on + strand, within rrmA at 1904.897 kb on - strand, within rrmA at 1904.988 kb on - strand, within rrmA at 1905.238 kb on - strand at 1905.238 kb on - strand at 1905.348 kb on + strand, within cspC at 1905.364 kb on - strand, within cspC at 1905.432 kb on + strand, within cspC at 1905.432 kb on + strand, within cspC at 1905.534 kb on - strand, within yobF at 1905.534 kb on - strand, within yobF at 1905.536 kb on + strand, within yobF at 1905.562 kb on + strand, within yobF at 1905.562 kb on + strand, within yobF at 1905.613 kb on + strand at 1905.619 kb on - strand at 1905.629 kb on - strand at 1905.647 kb on + strand at 1905.751 kb on - strand at 1905.751 kb on - strand at 1905.771 kb on + strand at 1905.782 kb on + strand at 1905.879 kb on - strand at 1905.881 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 1,903,496 - +0.0 1,903,498 - -1.6 1,903,529 + -0.9 1,903,537 - +0.7 1,903,548 + +0.2 1,903,553 + +0.4 1,903,558 + +1.8 1,903,558 + -2.0 1,903,583 + +0.0 1,903,602 - -0.4 1,903,602 - +1.5 1,903,639 - -0.0 1,903,654 - -0.1 1,903,702 + +1.1 1,903,777 - b1821 b1821 0.11 +0.7 1,903,777 - b1821 b1821 0.11 -0.1 1,904,065 - b1821 b1821 0.62 +0.0 1,904,087 - b1821 b1821 0.66 -1.2 1,904,603 + rrmA b1822 0.40 -0.9 1,904,603 + rrmA b1822 0.40 -0.8 1,904,691 + rrmA b1822 0.51 +0.8 1,904,897 - rrmA b1822 0.77 +0.8 1,904,988 - rrmA b1822 0.88 -2.8 1,905,238 - +1.4 1,905,238 - +1.5 1,905,348 + cspC b1823 0.47 +0.6 1,905,364 - cspC b1823 0.54 +0.0 1,905,432 + cspC b1823 0.87 +0.6 1,905,432 + cspC b1823 0.87 -0.3 1,905,534 - yobF b1824 0.43 +0.1 1,905,534 - yobF b1824 0.43 +1.6 1,905,536 + yobF b1824 0.44 -1.3 1,905,562 + yobF b1824 0.62 +0.4 1,905,562 + yobF b1824 0.62 +0.5 1,905,613 + -0.6 1,905,619 - +0.5 1,905,629 - +1.7 1,905,647 + -2.4 1,905,751 - +0.8 1,905,751 - -0.8 1,905,771 + -0.8 1,905,782 + -0.1 1,905,879 - +0.3 1,905,881 - +0.8
Or see this region's nucleotide sequence