Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydjX and ydjY are separated by 4 nucleotides ydjY and ydjZ are separated by 14 nucleotides ydjZ and ynjA overlap by 1 nucleotides ynjA and b1754 are separated by 9 nucleotides
b1750: ydjX - orf, hypothetical protein (VIMSS), at 1,831,425 to 1,832,135
ydjX
b1751: ydjY - orf, hypothetical protein (VIMSS), at 1,832,140 to 1,832,817
ydjY
b1752: ydjZ - conserved inner membrane protein (NCBI), at 1,832,832 to 1,833,539
ydjZ
b1753: ynjA - hypothetical protein (NCBI), at 1,833,539 to 1,834,087
ynjA
b1754: b1754 - orf, hypothetical protein (VIMSS), at 1,834,097 to 1,835,263
b1754
Position (kb)
1832
1833
1834 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1831.834 kb on + strand, within ydjX at 1831.992 kb on - strand, within ydjX at 1832.016 kb on + strand, within ydjX at 1832.016 kb on + strand, within ydjX at 1832.084 kb on - strand at 1832.113 kb on - strand at 1832.113 kb on - strand at 1832.187 kb on + strand at 1832.403 kb on + strand, within ydjY at 1832.456 kb on + strand, within ydjY at 1832.456 kb on + strand, within ydjY at 1832.456 kb on + strand, within ydjY at 1832.456 kb on + strand, within ydjY at 1832.475 kb on - strand, within ydjY at 1832.475 kb on - strand, within ydjY at 1832.682 kb on + strand, within ydjY at 1832.707 kb on - strand, within ydjY at 1833.245 kb on + strand, within ydjZ at 1833.394 kb on - strand, within ydjZ at 1833.430 kb on - strand, within ydjZ at 1833.789 kb on + strand, within ynjA at 1833.789 kb on + strand, within ynjA at 1834.210 kb on + strand at 1834.332 kb on - strand, within b1754 at 1834.427 kb on + strand, within b1754
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 1,831,834 + ydjX b1750 0.58 +1.1 1,831,992 - ydjX b1750 0.80 +0.2 1,832,016 + ydjX b1750 0.83 +0.3 1,832,016 + ydjX b1750 0.83 -1.1 1,832,084 - -0.1 1,832,113 - -0.3 1,832,113 - +0.7 1,832,187 + -1.1 1,832,403 + ydjY b1751 0.39 +0.7 1,832,456 + ydjY b1751 0.47 -0.3 1,832,456 + ydjY b1751 0.47 -0.3 1,832,456 + ydjY b1751 0.47 -0.1 1,832,456 + ydjY b1751 0.47 -1.6 1,832,475 - ydjY b1751 0.49 -1.3 1,832,475 - ydjY b1751 0.49 +0.5 1,832,682 + ydjY b1751 0.80 +2.4 1,832,707 - ydjY b1751 0.84 -1.1 1,833,245 + ydjZ b1752 0.58 -3.3 1,833,394 - ydjZ b1752 0.79 -0.4 1,833,430 - ydjZ b1752 0.84 +0.1 1,833,789 + ynjA b1753 0.46 +0.9 1,833,789 + ynjA b1753 0.46 -0.3 1,834,210 + -1.3 1,834,332 - b1754 b1754 0.20 +0.4 1,834,427 + b1754 b1754 0.28 +1.5
Or see this region's nucleotide sequence