Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pinQ and tfaQ are separated by 97 nucleotides tfaQ and stfQ overlap by 1 nucleotides stfQ and nohA overlap by 8 nucleotides nohA and ynfO are separated by 388 nucleotides
b1545: pinQ - Qin prophage; predicted site-specific recombinase (NCBI), at 1,631,646 to 1,632,236
pinQ
b1546: tfaQ - Qin prophage; predicted tail fibre assembly protein (NCBI), at 1,632,334 to 1,632,909
tfaQ
b1547: stfQ - Qin prophage; predicted side tail fibre assembly protein (NCBI), at 1,632,909 to 1,633,871
stfQ
b1548: nohA - Qin prophage; predicted packaging protein (RefSeq), at 1,633,864 to 1,634,391
nohA
b4533: ynfO - Qin prophage; predicted protein (NCBI), at 1,634,780 to 1,635,013
ynfO
Position (kb)
1632
1633
1634 Strain fitness (log2 ratio)
-1
0
1 at 1632.142 kb on - strand, within pinQ at 1632.995 kb on + strand at 1633.092 kb on - strand, within stfQ at 1633.234 kb on + strand, within stfQ at 1633.234 kb on + strand, within stfQ at 1633.282 kb on - strand, within stfQ at 1633.282 kb on - strand, within stfQ at 1633.282 kb on - strand, within stfQ at 1633.716 kb on + strand, within stfQ at 1633.724 kb on - strand, within stfQ at 1633.724 kb on - strand, within stfQ at 1633.829 kb on - strand at 1634.153 kb on + strand, within nohA at 1634.153 kb on + strand, within nohA
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 1,632,142 - pinQ b1545 0.84 -1.1 1,632,995 + +1.0 1,633,092 - stfQ b1547 0.19 -0.9 1,633,234 + stfQ b1547 0.34 +0.8 1,633,234 + stfQ b1547 0.34 -1.3 1,633,282 - stfQ b1547 0.39 -1.4 1,633,282 - stfQ b1547 0.39 -1.4 1,633,282 - stfQ b1547 0.39 +0.6 1,633,716 + stfQ b1547 0.84 -0.7 1,633,724 - stfQ b1547 0.85 -1.0 1,633,724 - stfQ b1547 0.85 -1.4 1,633,829 - +1.2 1,634,153 + nohA b1548 0.55 -0.5 1,634,153 + nohA b1548 0.55 -0.3
Or see this region's nucleotide sequence