Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mviN and flgN are separated by 39 nucleotides flgN and flgM are separated by 4 nucleotides flgM and flgA are separated by 75 nucleotides
b1069: mviN - predicted inner membrane protein (NCBI), at 1,127,062 to 1,128,597
mviN
b1070: flgN - export chaperone for FlgK and FlgL (NCBI), at 1,128,637 to 1,129,053
flgN
b1071: flgM - anti-sigma factor for FliA (sigma 28) (NCBI), at 1,129,058 to 1,129,351
flgM
b1072: flgA - flagellar basal body P-ring biosynthesis protein A (NCBI), at 1,129,427 to 1,130,086
flgA
Position (kb)
1128
1129
1130 Strain fitness (log2 ratio)
-2
-1
0
1 at 1128.553 kb on + strand at 1128.592 kb on + strand at 1128.600 kb on - strand at 1128.612 kb on + strand at 1128.932 kb on - strand, within flgN at 1129.057 kb on + strand at 1129.094 kb on - strand, within flgM at 1129.303 kb on + strand, within flgM at 1129.414 kb on - strand at 1129.433 kb on - strand at 1129.433 kb on - strand at 1129.579 kb on + strand, within flgA at 1129.663 kb on + strand, within flgA at 1129.716 kb on + strand, within flgA at 1129.771 kb on + strand, within flgA at 1129.771 kb on + strand, within flgA at 1129.810 kb on + strand, within flgA at 1129.811 kb on - strand, within flgA at 1129.813 kb on + strand, within flgA at 1129.815 kb on + strand, within flgA at 1129.815 kb on + strand, within flgA at 1129.958 kb on + strand, within flgA at 1130.045 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 1,128,553 + +1.6 1,128,592 + +1.1 1,128,600 - +0.4 1,128,612 + +1.6 1,128,932 - flgN b1070 0.71 -2.6 1,129,057 + -0.6 1,129,094 - flgM b1071 0.12 -2.3 1,129,303 + flgM b1071 0.83 -0.5 1,129,414 - -0.2 1,129,433 - -1.2 1,129,433 - -1.2 1,129,579 + flgA b1072 0.23 +1.4 1,129,663 + flgA b1072 0.36 -2.5 1,129,716 + flgA b1072 0.44 +1.3 1,129,771 + flgA b1072 0.52 -0.0 1,129,771 + flgA b1072 0.52 +0.3 1,129,810 + flgA b1072 0.58 -0.7 1,129,811 - flgA b1072 0.58 -0.5 1,129,813 + flgA b1072 0.58 +1.1 1,129,815 + flgA b1072 0.59 +0.8 1,129,815 + flgA b1072 0.59 -2.2 1,129,958 + flgA b1072 0.80 -0.0 1,130,045 + -0.7
Or see this region's nucleotide sequence