Strain Fitness in Escherichia coli BW25113 around b1014

Experiment: Chloramphenicol 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb1012 and ycdC are separated by 230 nucleotidesycdC and putA are separated by 39 nucleotidesputA and putP are separated by 422 nucleotides b1012: b1012 - orf, hypothetical protein (VIMSS), at 1,072,086 to 1,073,234 b1012 b1013: ycdC - predicted DNA-binding transcriptional regulator (NCBI), at 1,073,465 to 1,074,103 ycdC b1014: putA - fused DNA-binding transcriptional regulator/proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase (NCBI), at 1,074,143 to 1,078,105 putA b1015: putP - proline:sodium symporter (NCBI), at 1,078,528 to 1,080,036 putP Position (kb) 1074 1075 1076 1077 1078 1079Strain fitness (log2 ratio) -2 -1 0 1 2at 1073.238 kb on + strandat 1073.585 kb on - strand, within ycdCat 1073.585 kb on - strand, within ycdCat 1073.819 kb on - strand, within ycdCat 1073.908 kb on + strand, within ycdCat 1074.009 kb on - strand, within ycdCat 1074.073 kb on + strandat 1074.086 kb on - strandat 1074.088 kb on + strandat 1074.088 kb on + strandat 1074.126 kb on - strandat 1074.140 kb on + strandat 1074.195 kb on - strandat 1074.215 kb on - strandat 1074.218 kb on - strandat 1074.218 kb on - strandat 1074.294 kb on + strandat 1074.306 kb on + strandat 1074.438 kb on + strandat 1074.438 kb on + strandat 1074.499 kb on + strandat 1074.529 kb on - strandat 1074.529 kb on - strandat 1074.602 kb on - strand, within putAat 1074.714 kb on + strand, within putAat 1074.790 kb on + strand, within putAat 1074.794 kb on + strand, within putAat 1074.794 kb on + strand, within putAat 1074.847 kb on + strand, within putAat 1074.885 kb on + strand, within putAat 1074.885 kb on + strand, within putAat 1074.904 kb on + strand, within putAat 1074.904 kb on + strand, within putAat 1075.016 kb on + strand, within putAat 1075.031 kb on + strand, within putAat 1075.152 kb on + strand, within putAat 1075.152 kb on + strand, within putAat 1075.152 kb on + strand, within putAat 1075.169 kb on + strand, within putAat 1075.371 kb on - strand, within putAat 1075.385 kb on + strand, within putAat 1075.390 kb on + strand, within putAat 1075.408 kb on + strand, within putAat 1075.481 kb on + strand, within putAat 1075.481 kb on + strand, within putAat 1075.557 kb on - strand, within putAat 1075.557 kb on - strand, within putAat 1075.568 kb on + strand, within putAat 1075.596 kb on + strand, within putAat 1075.596 kb on + strand, within putAat 1075.789 kb on + strand, within putAat 1075.853 kb on - strand, within putAat 1075.878 kb on - strand, within putAat 1075.878 kb on - strand, within putAat 1076.079 kb on + strand, within putAat 1076.086 kb on + strand, within putAat 1076.109 kb on + strand, within putAat 1076.190 kb on - strand, within putAat 1076.205 kb on - strand, within putAat 1076.311 kb on - strand, within putAat 1076.311 kb on - strand, within putAat 1076.359 kb on - strand, within putAat 1076.359 kb on - strand, within putAat 1076.424 kb on - strand, within putAat 1076.426 kb on + strand, within putAat 1076.583 kb on - strand, within putAat 1076.729 kb on - strand, within putAat 1076.842 kb on - strand, within putAat 1076.905 kb on + strand, within putAat 1076.923 kb on - strand, within putAat 1076.997 kb on - strand, within putAat 1076.997 kb on - strand, within putAat 1077.025 kb on - strand, within putAat 1077.085 kb on - strand, within putAat 1077.092 kb on + strand, within putAat 1077.136 kb on + strand, within putAat 1077.136 kb on + strand, within putAat 1077.139 kb on - strand, within putAat 1077.193 kb on + strand, within putAat 1077.210 kb on - strand, within putAat 1077.298 kb on - strand, within putAat 1077.349 kb on - strand, within putAat 1077.382 kb on - strand, within putAat 1077.479 kb on - strand, within putAat 1077.506 kb on - strand, within putAat 1077.506 kb on - strand, within putAat 1077.664 kb on - strand, within putAat 1077.664 kb on - strand, within putAat 1077.670 kb on - strand, within putAat 1077.670 kb on - strand, within putAat 1077.772 kb on - strandat 1077.874 kb on - strandat 1077.874 kb on - strandat 1077.880 kb on - strandat 1077.889 kb on - strandat 1077.912 kb on - strandat 1077.938 kb on - strandat 1077.953 kb on + strandat 1078.020 kb on + strandat 1078.086 kb on - strandat 1078.086 kb on - strandat 1078.144 kb on - strandat 1078.154 kb on - strandat 1078.162 kb on - strandat 1078.206 kb on - strandat 1078.229 kb on - strandat 1078.254 kb on + strandat 1078.333 kb on + strandat 1078.333 kb on + strandat 1078.395 kb on - strandat 1078.401 kb on + strandat 1078.401 kb on + strandat 1078.421 kb on + strandat 1078.435 kb on + strandat 1078.443 kb on - strandat 1078.488 kb on + strandat 1078.531 kb on + strandat 1078.531 kb on + strandat 1078.544 kb on - strandat 1078.549 kb on - strandat 1078.582 kb on + strandat 1078.592 kb on + strandat 1078.620 kb on - strandat 1078.651 kb on - strandat 1078.651 kb on - strandat 1078.651 kb on - strandat 1078.679 kb on - strand, within putPat 1078.689 kb on + strand, within putPat 1078.712 kb on - strand, within putPat 1078.771 kb on - strand, within putPat 1078.788 kb on + strand, within putPat 1078.788 kb on + strand, within putPat 1078.845 kb on + strand, within putPat 1078.902 kb on + strand, within putPat 1078.902 kb on + strand, within putPat 1078.958 kb on + strand, within putPat 1079.051 kb on - strand, within putPat 1079.051 kb on - strand, within putPat 1079.087 kb on + strand, within putPat 1079.100 kb on - strand, within putP

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Per-strain Table

Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml
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1,073,238 + +0.2
1,073,585 - ycdC b1013 0.19 -0.4
1,073,585 - ycdC b1013 0.19 -1.7
1,073,819 - ycdC b1013 0.55 -2.6
1,073,908 + ycdC b1013 0.69 +0.9
1,074,009 - ycdC b1013 0.85 -0.9
1,074,073 + -0.2
1,074,086 - -0.1
1,074,088 + +1.1
1,074,088 + +0.9
1,074,126 - +0.8
1,074,140 + +0.3
1,074,195 - +0.5
1,074,215 - +0.4
1,074,218 - -0.7
1,074,218 - -1.1
1,074,294 + -0.7
1,074,306 + +1.0
1,074,438 + +0.1
1,074,438 + +1.2
1,074,499 + -0.4
1,074,529 - -0.1
1,074,529 - -2.3
1,074,602 - putA b1014 0.12 -0.2
1,074,714 + putA b1014 0.14 +0.3
1,074,790 + putA b1014 0.16 +1.1
1,074,794 + putA b1014 0.16 +0.7
1,074,794 + putA b1014 0.16 +0.9
1,074,847 + putA b1014 0.18 +0.1
1,074,885 + putA b1014 0.19 +0.2
1,074,885 + putA b1014 0.19 +1.5
1,074,904 + putA b1014 0.19 -2.1
1,074,904 + putA b1014 0.19 +1.4
1,075,016 + putA b1014 0.22 -0.7
1,075,031 + putA b1014 0.22 +0.9
1,075,152 + putA b1014 0.25 -0.7
1,075,152 + putA b1014 0.25 +2.2
1,075,152 + putA b1014 0.25 -0.1
1,075,169 + putA b1014 0.26 +0.9
1,075,371 - putA b1014 0.31 +0.7
1,075,385 + putA b1014 0.31 -0.2
1,075,390 + putA b1014 0.31 -0.1
1,075,408 + putA b1014 0.32 -0.7
1,075,481 + putA b1014 0.34 -1.7
1,075,481 + putA b1014 0.34 +0.9
1,075,557 - putA b1014 0.36 -0.7
1,075,557 - putA b1014 0.36 -0.4
1,075,568 + putA b1014 0.36 +0.5
1,075,596 + putA b1014 0.37 +0.9
1,075,596 + putA b1014 0.37 +0.2
1,075,789 + putA b1014 0.42 +0.9
1,075,853 - putA b1014 0.43 +0.9
1,075,878 - putA b1014 0.44 -1.4
1,075,878 - putA b1014 0.44 +0.9
1,076,079 + putA b1014 0.49 +0.2
1,076,086 + putA b1014 0.49 +0.1
1,076,109 + putA b1014 0.50 +0.7
1,076,190 - putA b1014 0.52 -0.0
1,076,205 - putA b1014 0.52 +0.9
1,076,311 - putA b1014 0.55 -1.1
1,076,311 - putA b1014 0.55 -0.7
1,076,359 - putA b1014 0.56 -1.1
1,076,359 - putA b1014 0.56 +0.5
1,076,424 - putA b1014 0.58 +0.4
1,076,426 + putA b1014 0.58 -1.4
1,076,583 - putA b1014 0.62 -0.9
1,076,729 - putA b1014 0.65 +0.1
1,076,842 - putA b1014 0.68 +0.9
1,076,905 + putA b1014 0.70 -1.7
1,076,923 - putA b1014 0.70 -0.8
1,076,997 - putA b1014 0.72 +0.7
1,076,997 - putA b1014 0.72 -1.7
1,077,025 - putA b1014 0.73 +0.5
1,077,085 - putA b1014 0.74 -0.3
1,077,092 + putA b1014 0.74 -1.1
1,077,136 + putA b1014 0.76 +2.2
1,077,136 + putA b1014 0.76 +0.7
1,077,139 - putA b1014 0.76 -0.1
1,077,193 + putA b1014 0.77 -0.7
1,077,210 - putA b1014 0.77 +0.4
1,077,298 - putA b1014 0.80 -2.4
1,077,349 - putA b1014 0.81 +0.7
1,077,382 - putA b1014 0.82 +1.5
1,077,479 - putA b1014 0.84 +0.6
1,077,506 - putA b1014 0.85 +0.8
1,077,506 - putA b1014 0.85 +0.2
1,077,664 - putA b1014 0.89 +0.5
1,077,664 - putA b1014 0.89 +0.2
1,077,670 - putA b1014 0.89 +1.1
1,077,670 - putA b1014 0.89 +0.2
1,077,772 - -2.1
1,077,874 - +1.9
1,077,874 - -1.4
1,077,880 - +0.4
1,077,889 - -0.1
1,077,912 - +0.3
1,077,938 - +1.1
1,077,953 + -0.5
1,078,020 + +0.9
1,078,086 - -0.1
1,078,086 - -0.1
1,078,144 - -2.4
1,078,154 - +0.2
1,078,162 - +0.9
1,078,206 - -1.0
1,078,229 - +0.2
1,078,254 + +1.1
1,078,333 + -1.3
1,078,333 + +0.7
1,078,395 - -1.1
1,078,401 + -0.7
1,078,401 + +1.1
1,078,421 + -0.5
1,078,435 + -1.4
1,078,443 - +0.4
1,078,488 + -1.1
1,078,531 + -1.3
1,078,531 + -1.1
1,078,544 - +0.6
1,078,549 - -0.7
1,078,582 + +2.3
1,078,592 + +0.5
1,078,620 - -0.3
1,078,651 - +1.9
1,078,651 - -1.1
1,078,651 - -0.1
1,078,679 - putP b1015 0.10 -0.3
1,078,689 + putP b1015 0.11 +0.8
1,078,712 - putP b1015 0.12 +0.5
1,078,771 - putP b1015 0.16 -2.3
1,078,788 + putP b1015 0.17 +0.6
1,078,788 + putP b1015 0.17 +1.3
1,078,845 + putP b1015 0.21 +0.1
1,078,902 + putP b1015 0.25 +1.8
1,078,902 + putP b1015 0.25 +0.9
1,078,958 + putP b1015 0.28 +0.4
1,079,051 - putP b1015 0.35 +0.4
1,079,051 - putP b1015 0.35 -1.6
1,079,087 + putP b1015 0.37 -0.7
1,079,100 - putP b1015 0.38 +0.6

Or see this region's nucleotide sequence