Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyaC and hyaD overlap by 4 nucleotides hyaD and hyaE overlap by 4 nucleotides hyaE and hyaF overlap by 4 nucleotides hyaF and appC are separated by 133 nucleotides
b0974: hyaC - hydrogenase 1, b-type cytochrome subunit (NCBI), at 1,034,289 to 1,034,996
hyaC
b0975: hyaD - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,034,993 to 1,035,580
hyaD
b0976: hyaE - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,035,577 to 1,035,975
hyaE
b0977: hyaF - protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI), at 1,035,972 to 1,036,829
hyaF
b0978: appC - cytochrome bd-II oxidase, subunit I (NCBI), at 1,036,963 to 1,038,507
appC
Position (kb)
1035
1036 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1034.577 kb on - strand, within hyaC at 1034.720 kb on - strand, within hyaC at 1034.720 kb on - strand, within hyaC at 1034.890 kb on - strand, within hyaC at 1034.895 kb on - strand, within hyaC at 1034.940 kb on + strand at 1034.940 kb on + strand at 1034.945 kb on + strand at 1034.947 kb on - strand at 1035.102 kb on + strand, within hyaD at 1035.201 kb on + strand, within hyaD at 1035.201 kb on + strand, within hyaD at 1035.291 kb on - strand, within hyaD at 1035.291 kb on - strand, within hyaD at 1035.310 kb on + strand, within hyaD at 1035.437 kb on + strand, within hyaD at 1035.437 kb on + strand, within hyaD at 1035.495 kb on + strand, within hyaD at 1035.503 kb on - strand, within hyaD at 1035.503 kb on - strand, within hyaD at 1035.803 kb on + strand, within hyaE at 1035.813 kb on + strand, within hyaE at 1035.892 kb on + strand, within hyaE at 1035.910 kb on - strand, within hyaE at 1035.910 kb on - strand, within hyaE at 1035.990 kb on + strand at 1036.062 kb on - strand, within hyaF at 1036.216 kb on + strand, within hyaF at 1036.216 kb on + strand, within hyaF at 1036.275 kb on + strand, within hyaF at 1036.294 kb on + strand, within hyaF at 1036.394 kb on - strand, within hyaF at 1036.463 kb on + strand at 1036.690 kb on - strand, within hyaF at 1036.690 kb on - strand, within hyaF at 1036.774 kb on - strand at 1036.791 kb on - strand at 1036.863 kb on - strand at 1036.863 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 1,034,577 - hyaC b0974 0.41 +0.5 1,034,720 - hyaC b0974 0.61 +0.3 1,034,720 - hyaC b0974 0.61 -2.1 1,034,890 - hyaC b0974 0.85 -1.1 1,034,895 - hyaC b0974 0.86 +0.1 1,034,940 + -1.1 1,034,940 + +2.1 1,034,945 + -1.1 1,034,947 - -1.3 1,035,102 + hyaD b0975 0.19 +0.3 1,035,201 + hyaD b0975 0.35 -0.1 1,035,201 + hyaD b0975 0.35 +2.9 1,035,291 - hyaD b0975 0.51 -1.1 1,035,291 - hyaD b0975 0.51 +0.6 1,035,310 + hyaD b0975 0.54 -0.8 1,035,437 + hyaD b0975 0.76 +1.3 1,035,437 + hyaD b0975 0.76 -0.7 1,035,495 + hyaD b0975 0.85 +0.2 1,035,503 - hyaD b0975 0.87 -1.4 1,035,503 - hyaD b0975 0.87 -1.4 1,035,803 + hyaE b0976 0.57 -0.2 1,035,813 + hyaE b0976 0.59 -0.5 1,035,892 + hyaE b0976 0.79 -2.6 1,035,910 - hyaE b0976 0.83 -0.4 1,035,910 - hyaE b0976 0.83 -0.1 1,035,990 + -0.6 1,036,062 - hyaF b0977 0.10 -0.5 1,036,216 + hyaF b0977 0.28 -1.3 1,036,216 + hyaF b0977 0.28 -0.1 1,036,275 + hyaF b0977 0.35 -0.3 1,036,294 + hyaF b0977 0.38 -0.7 1,036,394 - hyaF b0977 0.49 +0.3 1,036,463 + +0.9 1,036,690 - hyaF b0977 0.84 +0.9 1,036,690 - hyaF b0977 0.84 +0.6 1,036,774 - -0.3 1,036,791 - -1.1 1,036,863 - -0.1 1,036,863 - -1.6
Or see this region's nucleotide sequence