Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycaR and kdsB overlap by 4 nucleotides kdsB and ycbJ are separated by 153 nucleotides ycbJ and ycbC overlap by 24 nucleotides ycbC and smtA are separated by 135 nucleotides
b0917: ycaR - hypothetical protein (NCBI), at 969,896 to 970,078
ycaR
b0918: kdsB - 3-deoxy-manno-octulosonate cytidylyltransferase (NCBI), at 970,075 to 970,821
kdsB
b0919: ycbJ - hypothetical protein (NCBI), at 970,975 to 971,868
ycbJ
b0920: ycbC - conserved inner membrane protein (NCBI), at 971,845 to 972,624
ycbC
b0921: smtA - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 972,760 to 973,545
smtA
Position (kb) 970
971
972 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 970.868 kb on - strand at 970.868 kb on - strand at 971.000 kb on + strand at 971.061 kb on + strand at 971.071 kb on + strand, within ycbJ at 971.221 kb on - strand, within ycbJ at 971.291 kb on + strand, within ycbJ at 971.291 kb on + strand, within ycbJ at 971.300 kb on + strand, within ycbJ at 971.300 kb on + strand, within ycbJ at 971.348 kb on - strand, within ycbJ at 971.348 kb on - strand, within ycbJ at 971.350 kb on + strand, within ycbJ at 971.382 kb on + strand, within ycbJ at 971.382 kb on + strand, within ycbJ at 971.426 kb on + strand, within ycbJ at 971.434 kb on - strand, within ycbJ at 971.436 kb on + strand, within ycbJ at 971.442 kb on + strand, within ycbJ at 971.442 kb on + strand, within ycbJ at 971.447 kb on + strand, within ycbJ at 971.447 kb on + strand, within ycbJ at 971.447 kb on + strand, within ycbJ at 971.706 kb on + strand, within ycbJ at 972.023 kb on - strand, within ycbC at 972.065 kb on - strand, within ycbC at 972.215 kb on - strand, within ycbC at 972.313 kb on - strand, within ycbC at 972.351 kb on - strand, within ycbC at 972.354 kb on + strand, within ycbC at 972.354 kb on + strand, within ycbC at 972.535 kb on + strand, within ycbC at 972.548 kb on - strand at 972.819 kb on + strand at 972.852 kb on + strand, within smtA
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 970,868 - +0.0 970,868 - -2.2 971,000 + -0.1 971,061 + -0.7 971,071 + ycbJ b0919 0.11 +3.5 971,221 - ycbJ b0919 0.28 -0.5 971,291 + ycbJ b0919 0.35 +0.5 971,291 + ycbJ b0919 0.35 +1.3 971,300 + ycbJ b0919 0.36 +0.5 971,300 + ycbJ b0919 0.36 -0.7 971,348 - ycbJ b0919 0.42 -0.1 971,348 - ycbJ b0919 0.42 -0.8 971,350 + ycbJ b0919 0.42 -0.3 971,382 + ycbJ b0919 0.46 +2.2 971,382 + ycbJ b0919 0.46 +1.9 971,426 + ycbJ b0919 0.50 +0.9 971,434 - ycbJ b0919 0.51 +1.3 971,436 + ycbJ b0919 0.52 +1.6 971,442 + ycbJ b0919 0.52 -1.7 971,442 + ycbJ b0919 0.52 +0.9 971,447 + ycbJ b0919 0.53 -0.1 971,447 + ycbJ b0919 0.53 +0.5 971,447 + ycbJ b0919 0.53 +1.8 971,706 + ycbJ b0919 0.82 +1.6 972,023 - ycbC b0920 0.23 -0.5 972,065 - ycbC b0920 0.28 -0.2 972,215 - ycbC b0920 0.47 +0.2 972,313 - ycbC b0920 0.60 +0.8 972,351 - ycbC b0920 0.65 -2.7 972,354 + ycbC b0920 0.65 -1.1 972,354 + ycbC b0920 0.65 +1.5 972,535 + ycbC b0920 0.88 +0.5 972,548 - -1.2 972,819 + -0.1 972,852 + smtA b0921 0.12 -0.6
Or see this region's nucleotide sequence