Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybbC and ylbH are separated by 2 nucleotides ylbH and ybbD overlap by 20 nucleotides ybbD and ylbI are separated by 369 nucleotides ylbI and ylbG are separated by 2 nucleotides
b0498: ybbC - hypothetical protein (NCBI), at 526,805 to 527,173
ybbC
b0499: ylbH - conserved protein, rhs-like (RefSeq), at 527,176 to 527,883
ylbH
b0500: ybbD - hypothetical protein (RefSeq), at 527,864 to 528,354
ybbD
b4632: ylbI - no description, at 528,724 to 528,816
ylbI
b0502: ylbG - no description, at 528,819 to 529,240
ylbG
Position (kb) 527
528
529 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 526.871 kb on + strand, within ybbC at 527.187 kb on - strand at 527.214 kb on - strand at 527.219 kb on - strand at 527.222 kb on + strand at 527.227 kb on - strand at 527.241 kb on + strand at 527.256 kb on + strand, within ylbH at 527.256 kb on + strand, within ylbH at 527.263 kb on + strand, within ylbH at 527.268 kb on + strand, within ylbH at 527.276 kb on - strand, within ylbH at 527.291 kb on - strand, within ylbH at 527.298 kb on + strand, within ylbH at 527.309 kb on + strand, within ylbH at 527.309 kb on + strand, within ylbH at 527.322 kb on - strand, within ylbH at 527.342 kb on - strand, within ylbH at 527.342 kb on - strand, within ylbH at 527.345 kb on + strand, within ylbH at 527.361 kb on + strand, within ylbH at 527.361 kb on + strand, within ylbH at 527.361 kb on + strand, within ylbH at 527.361 kb on + strand, within ylbH at 527.363 kb on + strand, within ylbH at 527.365 kb on + strand, within ylbH at 527.365 kb on + strand, within ylbH at 527.371 kb on - strand, within ylbH at 527.371 kb on - strand, within ylbH at 527.373 kb on + strand, within ylbH at 527.373 kb on + strand, within ylbH at 527.373 kb on + strand, within ylbH at 527.378 kb on + strand, within ylbH at 527.378 kb on + strand, within ylbH at 527.383 kb on + strand, within ylbH at 527.383 kb on + strand, within ylbH at 527.384 kb on + strand, within ylbH at 527.391 kb on - strand, within ylbH at 527.396 kb on + strand, within ylbH at 527.400 kb on + strand, within ylbH at 527.406 kb on + strand, within ylbH at 527.406 kb on + strand, within ylbH at 527.406 kb on + strand, within ylbH at 527.406 kb on + strand, within ylbH at 527.406 kb on + strand, within ylbH at 527.412 kb on - strand, within ylbH at 527.413 kb on + strand, within ylbH at 527.418 kb on + strand, within ylbH at 527.430 kb on + strand, within ylbH at 527.435 kb on + strand, within ylbH at 527.435 kb on + strand, within ylbH at 527.435 kb on + strand, within ylbH at 527.435 kb on + strand, within ylbH at 527.443 kb on + strand, within ylbH at 527.444 kb on + strand, within ylbH at 527.446 kb on + strand, within ylbH at 527.459 kb on + strand, within ylbH at 527.465 kb on + strand, within ylbH at 527.465 kb on + strand, within ylbH at 527.513 kb on + strand, within ylbH at 527.517 kb on + strand, within ylbH at 527.723 kb on + strand, within ylbH at 528.146 kb on - strand, within ybbD at 528.146 kb on - strand, within ybbD at 528.328 kb on + strand at 528.377 kb on - strand at 528.384 kb on + strand at 528.446 kb on + strand at 528.454 kb on + strand at 528.502 kb on + strand at 528.502 kb on + strand at 528.750 kb on - strand, within ylbI at 528.757 kb on + strand, within ylbI at 528.757 kb on + strand, within ylbI at 528.759 kb on + strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.767 kb on - strand, within ylbI at 528.770 kb on - strand, within ylbI at 528.770 kb on - strand, within ylbI at 528.770 kb on - strand, within ylbI at 528.777 kb on + strand, within ylbI at 528.777 kb on + strand, within ylbI at 528.786 kb on - strand, within ylbI at 528.786 kb on - strand, within ylbI at 528.786 kb on - strand, within ylbI at 528.786 kb on - strand, within ylbI at 528.793 kb on - strand, within ylbI at 528.799 kb on - strand, within ylbI at 528.803 kb on - strand, within ylbI at 528.803 kb on - strand, within ylbI at 528.834 kb on - strand at 528.834 kb on - strand at 528.880 kb on + strand, within ylbG at 528.897 kb on - strand, within ylbG at 528.897 kb on - strand, within ylbG at 528.969 kb on + strand, within ylbG at 529.243 kb on + strand at 529.243 kb on + strand at 529.243 kb on + strand at 529.249 kb on - strand at 529.269 kb on + strand at 529.312 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 526,871 + ybbC b0498 0.18 -1.6 527,187 - +0.8 527,214 - -1.7 527,219 - -2.3 527,222 + -0.8 527,227 - -2.3 527,241 + +0.4 527,256 + ylbH b0499 0.11 +0.9 527,256 + ylbH b0499 0.11 -2.1 527,263 + ylbH b0499 0.12 +0.6 527,268 + ylbH b0499 0.13 -0.5 527,276 - ylbH b0499 0.14 -1.1 527,291 - ylbH b0499 0.16 +0.7 527,298 + ylbH b0499 0.17 +0.2 527,309 + ylbH b0499 0.19 -0.1 527,309 + ylbH b0499 0.19 -0.3 527,322 - ylbH b0499 0.21 -2.3 527,342 - ylbH b0499 0.23 +0.0 527,342 - ylbH b0499 0.23 -0.8 527,345 + ylbH b0499 0.24 +0.1 527,361 + ylbH b0499 0.26 +0.2 527,361 + ylbH b0499 0.26 -0.1 527,361 + ylbH b0499 0.26 -0.1 527,361 + ylbH b0499 0.26 +1.5 527,363 + ylbH b0499 0.26 -0.5 527,365 + ylbH b0499 0.27 -0.3 527,365 + ylbH b0499 0.27 +1.1 527,371 - ylbH b0499 0.28 +0.2 527,371 - ylbH b0499 0.28 -1.3 527,373 + ylbH b0499 0.28 +0.9 527,373 + ylbH b0499 0.28 +0.1 527,373 + ylbH b0499 0.28 -0.3 527,378 + ylbH b0499 0.29 +0.5 527,378 + ylbH b0499 0.29 +1.2 527,383 + ylbH b0499 0.29 +0.7 527,383 + ylbH b0499 0.29 -0.8 527,384 + ylbH b0499 0.29 -1.4 527,391 - ylbH b0499 0.30 +2.7 527,396 + ylbH b0499 0.31 +0.1 527,400 + ylbH b0499 0.32 +0.2 527,406 + ylbH b0499 0.32 +1.1 527,406 + ylbH b0499 0.32 +0.1 527,406 + ylbH b0499 0.32 -0.7 527,406 + ylbH b0499 0.32 +0.3 527,406 + ylbH b0499 0.32 -1.3 527,412 - ylbH b0499 0.33 +0.2 527,413 + ylbH b0499 0.33 -0.2 527,418 + ylbH b0499 0.34 -0.3 527,430 + ylbH b0499 0.36 -0.0 527,435 + ylbH b0499 0.37 -2.1 527,435 + ylbH b0499 0.37 +0.1 527,435 + ylbH b0499 0.37 +0.3 527,435 + ylbH b0499 0.37 +0.7 527,443 + ylbH b0499 0.38 +0.3 527,444 + ylbH b0499 0.38 +0.9 527,446 + ylbH b0499 0.38 +0.7 527,459 + ylbH b0499 0.40 -0.8 527,465 + ylbH b0499 0.41 +0.6 527,465 + ylbH b0499 0.41 -0.8 527,513 + ylbH b0499 0.48 +0.0 527,517 + ylbH b0499 0.48 -0.7 527,723 + ylbH b0499 0.77 -0.1 528,146 - ybbD b0500 0.57 -2.3 528,146 - ybbD b0500 0.57 -3.2 528,328 + -1.8 528,377 - -1.0 528,384 + -0.8 528,446 + +0.8 528,454 + -0.4 528,502 + -1.1 528,502 + +0.7 528,750 - ylbI b4632 0.28 +0.5 528,757 + ylbI b4632 0.35 -1.1 528,757 + ylbI b4632 0.35 -1.4 528,759 + ylbI b4632 0.38 +0.3 528,765 - ylbI b4632 0.44 -0.5 528,765 - ylbI b4632 0.44 +1.0 528,765 - ylbI b4632 0.44 +1.4 528,765 - ylbI b4632 0.44 +0.6 528,765 - ylbI b4632 0.44 -0.6 528,767 - ylbI b4632 0.46 -0.0 528,770 - ylbI b4632 0.49 -1.0 528,770 - ylbI b4632 0.49 -0.3 528,770 - ylbI b4632 0.49 +2.1 528,777 + ylbI b4632 0.57 -1.2 528,777 + ylbI b4632 0.57 +0.7 528,786 - ylbI b4632 0.67 +0.9 528,786 - ylbI b4632 0.67 -0.3 528,786 - ylbI b4632 0.67 +0.5 528,786 - ylbI b4632 0.67 +0.9 528,793 - ylbI b4632 0.74 -0.0 528,799 - ylbI b4632 0.81 +0.6 528,803 - ylbI b4632 0.85 +2.7 528,803 - ylbI b4632 0.85 -2.7 528,834 - +0.7 528,834 - -1.4 528,880 + ylbG b0502 0.14 -0.5 528,897 - ylbG b0502 0.18 +0.9 528,897 - ylbG b0502 0.18 +0.7 528,969 + ylbG b0502 0.36 -0.1 529,243 + -1.4 529,243 + +0.1 529,243 + -0.6 529,249 - -0.4 529,269 + -1.7 529,312 - +1.4
Or see this region's nucleotide sequence