Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cyoE and cyoD are separated by 11 nucleotides cyoD and cyoC overlap by 1 nucleotides cyoC and cyoB overlap by 11 nucleotides
b0428: cyoE - protoheme IX farnesyltransferase (NCBI), at 446,039 to 446,929
cyoE
b0429: cyoD - cytochrome o ubiquinol oxidase subunit IV (NCBI), at 446,941 to 447,270
cyoD
b0430: cyoC - cytochrome o ubiquinol oxidase subunit III (NCBI), at 447,270 to 447,884
cyoC
b0431: cyoB - cytochrome o ubiquinol oxidase subunit I (NCBI), at 447,874 to 449,865
cyoB
Position (kb) 446
447
448 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 445.978 kb on + strand at 446.028 kb on - strand at 446.028 kb on - strand at 446.054 kb on + strand at 446.069 kb on - strand at 446.069 kb on - strand at 446.096 kb on + strand at 446.101 kb on - strand at 446.119 kb on + strand at 446.140 kb on - strand, within cyoE at 446.161 kb on - strand, within cyoE at 446.242 kb on - strand, within cyoE at 446.297 kb on - strand, within cyoE at 446.394 kb on + strand, within cyoE at 446.394 kb on + strand, within cyoE at 446.394 kb on + strand, within cyoE at 446.407 kb on - strand, within cyoE at 446.525 kb on + strand, within cyoE at 446.525 kb on + strand, within cyoE at 446.533 kb on - strand, within cyoE at 446.562 kb on - strand, within cyoE at 446.562 kb on - strand, within cyoE at 446.657 kb on + strand, within cyoE at 446.657 kb on + strand, within cyoE at 446.673 kb on - strand, within cyoE at 446.677 kb on + strand, within cyoE at 446.677 kb on + strand, within cyoE at 446.796 kb on - strand, within cyoE at 446.824 kb on + strand, within cyoE at 446.832 kb on - strand, within cyoE at 446.880 kb on + strand at 446.880 kb on + strand at 446.932 kb on + strand at 446.944 kb on + strand at 446.981 kb on - strand, within cyoD at 447.018 kb on - strand, within cyoD at 447.093 kb on - strand, within cyoD at 447.093 kb on - strand, within cyoD at 447.105 kb on - strand, within cyoD at 447.161 kb on - strand, within cyoD at 447.167 kb on + strand, within cyoD at 447.175 kb on - strand, within cyoD at 447.201 kb on + strand, within cyoD at 447.223 kb on - strand, within cyoD at 447.285 kb on - strand at 447.303 kb on - strand at 447.347 kb on + strand, within cyoC at 447.351 kb on + strand, within cyoC at 447.373 kb on - strand, within cyoC at 447.407 kb on + strand, within cyoC at 447.418 kb on - strand, within cyoC at 447.445 kb on - strand, within cyoC at 447.492 kb on + strand, within cyoC at 447.492 kb on + strand, within cyoC at 447.521 kb on + strand, within cyoC at 447.570 kb on + strand, within cyoC at 447.580 kb on + strand, within cyoC at 447.679 kb on + strand, within cyoC at 447.956 kb on + strand at 447.964 kb on - strand at 447.964 kb on - strand at 447.989 kb on + strand at 448.002 kb on - strand at 448.005 kb on + strand at 448.092 kb on - strand, within cyoB at 448.092 kb on - strand, within cyoB at 448.134 kb on - strand, within cyoB at 448.134 kb on - strand, within cyoB at 448.238 kb on - strand, within cyoB
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 445,978 + +0.1 446,028 - -0.3 446,028 - +1.5 446,054 + +0.6 446,069 - +2.3 446,069 - -0.1 446,096 + -0.4 446,101 - -2.4 446,119 + -0.1 446,140 - cyoE b0428 0.11 +0.7 446,161 - cyoE b0428 0.14 -1.7 446,242 - cyoE b0428 0.23 +0.2 446,297 - cyoE b0428 0.29 -0.7 446,394 + cyoE b0428 0.40 +0.5 446,394 + cyoE b0428 0.40 -2.6 446,394 + cyoE b0428 0.40 -0.1 446,407 - cyoE b0428 0.41 -1.6 446,525 + cyoE b0428 0.55 +0.9 446,525 + cyoE b0428 0.55 -0.1 446,533 - cyoE b0428 0.55 +1.9 446,562 - cyoE b0428 0.59 -1.7 446,562 - cyoE b0428 0.59 -1.1 446,657 + cyoE b0428 0.69 -3.3 446,657 + cyoE b0428 0.69 -0.8 446,673 - cyoE b0428 0.71 +1.6 446,677 + cyoE b0428 0.72 +0.9 446,677 + cyoE b0428 0.72 -0.7 446,796 - cyoE b0428 0.85 -1.1 446,824 + cyoE b0428 0.88 -1.1 446,832 - cyoE b0428 0.89 +1.0 446,880 + +0.3 446,880 + +1.8 446,932 + -0.4 446,944 + +0.9 446,981 - cyoD b0429 0.12 -1.4 447,018 - cyoD b0429 0.23 -0.4 447,093 - cyoD b0429 0.46 -0.1 447,093 - cyoD b0429 0.46 -0.4 447,105 - cyoD b0429 0.50 -0.5 447,161 - cyoD b0429 0.67 +0.9 447,167 + cyoD b0429 0.68 -0.4 447,175 - cyoD b0429 0.71 -1.1 447,201 + cyoD b0429 0.79 -0.7 447,223 - cyoD b0429 0.85 +0.3 447,285 - -0.7 447,303 - +0.2 447,347 + cyoC b0430 0.13 -0.7 447,351 + cyoC b0430 0.13 -1.7 447,373 - cyoC b0430 0.17 +1.4 447,407 + cyoC b0430 0.22 -0.1 447,418 - cyoC b0430 0.24 +0.2 447,445 - cyoC b0430 0.28 -0.7 447,492 + cyoC b0430 0.36 +1.3 447,492 + cyoC b0430 0.36 -1.4 447,521 + cyoC b0430 0.41 -1.4 447,570 + cyoC b0430 0.49 +0.3 447,580 + cyoC b0430 0.50 +0.5 447,679 + cyoC b0430 0.67 -0.1 447,956 + -0.8 447,964 - -1.4 447,964 - +1.5 447,989 + +1.6 448,002 - +0.8 448,005 + -1.6 448,092 - cyoB b0431 0.11 +0.5 448,092 - cyoB b0431 0.11 +1.9 448,134 - cyoB b0431 0.13 +1.2 448,134 - cyoB b0431 0.13 +0.2 448,238 - cyoB b0431 0.18 -3.0
Or see this region's nucleotide sequence