Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt malZ and yajB are separated by 4 nucleotides yajB and queA are separated by 92 nucleotides
b0403: malZ - maltodextrin glucosidase (NCBI), at 421,742 to 423,556
malZ
b0404: yajB - hypothetical protein (NCBI), at 423,561 to 424,142
yajB
b0405: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI), at 424,235 to 425,305
queA
Position (kb) 423
424
425 Strain fitness (log2 ratio) -1
0
1
2 at 422.690 kb on - strand, within malZ at 422.699 kb on + strand, within malZ at 422.745 kb on + strand, within malZ at 422.753 kb on + strand, within malZ at 422.753 kb on + strand, within malZ at 422.795 kb on - strand, within malZ at 422.892 kb on - strand, within malZ at 422.892 kb on - strand, within malZ at 422.905 kb on + strand, within malZ at 422.909 kb on + strand, within malZ at 422.909 kb on + strand, within malZ at 422.968 kb on + strand, within malZ at 423.015 kb on - strand, within malZ at 423.025 kb on - strand, within malZ at 423.025 kb on - strand, within malZ at 423.071 kb on - strand, within malZ at 423.162 kb on + strand, within malZ at 423.283 kb on - strand, within malZ at 423.401 kb on - strand at 423.404 kb on - strand at 423.404 kb on - strand at 423.445 kb on - strand at 423.512 kb on + strand at 423.548 kb on - strand at 423.548 kb on - strand at 423.711 kb on + strand, within yajB at 423.711 kb on + strand, within yajB at 423.909 kb on + strand, within yajB at 423.909 kb on + strand at 423.923 kb on + strand, within yajB at 423.996 kb on + strand, within yajB at 424.011 kb on - strand, within yajB at 424.112 kb on + strand at 424.121 kb on - strand at 424.121 kb on - strand at 424.232 kb on + strand at 424.232 kb on + strand at 424.280 kb on + strand at 424.280 kb on + strand at 424.484 kb on - strand, within queA at 424.484 kb on - strand, within queA at 424.612 kb on + strand, within queA at 424.651 kb on - strand, within queA at 424.651 kb on - strand, within queA at 424.651 kb on - strand, within queA at 424.657 kb on + strand, within queA at 424.668 kb on + strand, within queA at 424.668 kb on + strand, within queA at 424.922 kb on - strand, within queA
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 422,690 - malZ b0403 0.52 +0.6 422,699 + malZ b0403 0.53 +0.0 422,745 + malZ b0403 0.55 -0.1 422,753 + malZ b0403 0.56 +1.8 422,753 + malZ b0403 0.56 +0.6 422,795 - malZ b0403 0.58 -1.1 422,892 - malZ b0403 0.63 +0.1 422,892 - malZ b0403 0.63 +1.1 422,905 + malZ b0403 0.64 +0.3 422,909 + malZ b0403 0.64 -0.4 422,909 + malZ b0403 0.64 +1.9 422,968 + malZ b0403 0.68 +0.7 423,015 - malZ b0403 0.70 -0.5 423,025 - malZ b0403 0.71 -1.7 423,025 - malZ b0403 0.71 -0.4 423,071 - malZ b0403 0.73 +0.7 423,162 + malZ b0403 0.78 +0.7 423,283 - malZ b0403 0.85 -0.1 423,401 - -1.6 423,404 - +0.5 423,404 - -1.1 423,445 - -0.1 423,512 + -0.1 423,548 - -1.1 423,548 - +0.2 423,711 + yajB b0404 0.26 -0.5 423,711 + yajB b0404 0.26 -0.9 423,909 + yajB b0404 0.60 -1.4 423,909 + +0.9 423,923 + yajB b0404 0.62 -0.5 423,996 + yajB b0404 0.75 -1.7 424,011 - yajB b0404 0.77 +0.9 424,112 + -1.2 424,121 - -0.3 424,121 - +0.9 424,232 + -1.4 424,232 + +0.5 424,280 + +1.3 424,280 + -0.3 424,484 - queA b0405 0.23 -0.6 424,484 - queA b0405 0.23 -0.4 424,612 + queA b0405 0.35 -0.1 424,651 - queA b0405 0.39 -0.7 424,651 - queA b0405 0.39 -0.7 424,651 - queA b0405 0.39 -0.0 424,657 + queA b0405 0.39 +0.6 424,668 + queA b0405 0.40 -1.1 424,668 + queA b0405 0.40 -0.6 424,922 - queA b0405 0.64 +1.2
Or see this region's nucleotide sequence