Experiment: Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt guaC and hofC are separated by 34 nucleotides hofC and hofB overlap by 11 nucleotides
b0104: guaC - guanosine 5'-monophosphate oxidoreductase (NCBI), at 113,444 to 114,487
guaC
b0106: hofC - assembly protein in type IV pilin biogenesis, transmembrane protein (NCBI), at 114,522 to 115,724
hofC
b0107: hofB - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 115,714 to 117,099
hofB
Position (kb)
114
115
116 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 113.564 kb on + strand, within guaC at 113.668 kb on + strand, within guaC at 113.668 kb on + strand, within guaC at 113.788 kb on + strand, within guaC at 113.788 kb on + strand, within guaC at 113.803 kb on + strand, within guaC at 113.803 kb on + strand, within guaC at 113.854 kb on + strand, within guaC at 114.020 kb on + strand, within guaC at 114.039 kb on + strand, within guaC at 114.075 kb on + strand, within guaC at 114.075 kb on + strand, within guaC at 114.082 kb on + strand, within guaC at 114.082 kb on + strand, within guaC at 114.121 kb on - strand, within guaC at 114.121 kb on - strand, within guaC at 114.191 kb on - strand, within guaC at 114.191 kb on - strand, within guaC at 114.217 kb on - strand, within guaC at 114.236 kb on - strand, within guaC at 114.246 kb on - strand, within guaC at 114.278 kb on - strand, within guaC at 114.310 kb on + strand, within guaC at 114.354 kb on - strand, within guaC at 114.399 kb on - strand at 114.422 kb on - strand at 114.480 kb on + strand at 114.480 kb on + strand at 114.509 kb on - strand at 114.509 kb on - strand at 114.519 kb on + strand at 114.524 kb on + strand at 114.527 kb on - strand at 114.527 kb on - strand at 114.532 kb on - strand at 114.535 kb on - strand at 114.545 kb on - strand at 114.562 kb on + strand at 114.627 kb on + strand at 114.634 kb on + strand at 114.680 kb on + strand, within hofC at 114.838 kb on + strand, within hofC at 114.838 kb on + strand, within hofC at 114.844 kb on + strand, within hofC at 114.928 kb on - strand, within hofC at 114.930 kb on - strand at 114.930 kb on - strand, within hofC at 114.998 kb on + strand, within hofC at 114.998 kb on + strand, within hofC at 115.010 kb on - strand, within hofC at 115.021 kb on + strand, within hofC at 115.021 kb on + strand, within hofC at 115.077 kb on - strand, within hofC at 115.152 kb on - strand, within hofC at 115.152 kb on - strand, within hofC at 115.201 kb on - strand, within hofC at 115.203 kb on - strand, within hofC at 115.203 kb on - strand, within hofC at 115.273 kb on + strand, within hofC at 115.278 kb on + strand, within hofC at 115.278 kb on + strand, within hofC at 115.330 kb on + strand, within hofC at 115.367 kb on + strand, within hofC at 115.367 kb on + strand, within hofC at 115.379 kb on + strand, within hofC at 115.379 kb on + strand, within hofC at 115.409 kb on + strand, within hofC at 115.474 kb on - strand, within hofC at 115.507 kb on + strand, within hofC at 115.507 kb on + strand, within hofC at 115.515 kb on - strand, within hofC at 115.624 kb on + strand at 115.651 kb on + strand at 115.699 kb on - strand at 115.737 kb on + strand at 115.856 kb on - strand, within hofB at 115.856 kb on - strand, within hofB at 115.910 kb on + strand, within hofB at 115.911 kb on - strand, within hofB at 115.911 kb on - strand, within hofB at 115.941 kb on + strand, within hofB at 115.980 kb on + strand, within hofB at 116.058 kb on + strand, within hofB at 116.149 kb on - strand, within hofB at 116.335 kb on + strand, within hofB at 116.399 kb on + strand, within hofB at 116.430 kb on - strand, within hofB at 116.430 kb on - strand, within hofB at 116.471 kb on - strand, within hofB
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.005 mg/ml remove 113,564 + guaC b0104 0.11 +0.6 113,668 + guaC b0104 0.21 +0.1 113,668 + guaC b0104 0.21 -0.2 113,788 + guaC b0104 0.33 -0.5 113,788 + guaC b0104 0.33 +0.7 113,803 + guaC b0104 0.34 -1.5 113,803 + guaC b0104 0.34 -3.1 113,854 + guaC b0104 0.39 -0.5 114,020 + guaC b0104 0.55 -0.1 114,039 + guaC b0104 0.57 +0.3 114,075 + guaC b0104 0.60 +0.7 114,075 + guaC b0104 0.60 +1.1 114,082 + guaC b0104 0.61 -2.4 114,082 + guaC b0104 0.61 +0.9 114,121 - guaC b0104 0.65 -1.8 114,121 - guaC b0104 0.65 -0.1 114,191 - guaC b0104 0.72 -1.6 114,191 - guaC b0104 0.72 +0.4 114,217 - guaC b0104 0.74 -0.9 114,236 - guaC b0104 0.76 +1.4 114,246 - guaC b0104 0.77 +1.6 114,278 - guaC b0104 0.80 -0.6 114,310 + guaC b0104 0.83 -0.7 114,354 - guaC b0104 0.87 +1.0 114,399 - +0.9 114,422 - -3.1 114,480 + +0.2 114,480 + -1.4 114,509 - -0.3 114,509 - -0.4 114,519 + -0.1 114,524 + -0.1 114,527 - +0.5 114,527 - +0.3 114,532 - +0.9 114,535 - -1.2 114,545 - -1.4 114,562 + -0.2 114,627 + +1.4 114,634 + -1.2 114,680 + hofC b0106 0.13 +0.3 114,838 + hofC b0106 0.26 -0.4 114,838 + hofC b0106 0.26 -1.1 114,844 + hofC b0106 0.27 -1.5 114,928 - hofC b0106 0.34 -1.8 114,930 - -0.1 114,930 - hofC b0106 0.34 +0.6 114,998 + hofC b0106 0.40 +0.1 114,998 + hofC b0106 0.40 +0.5 115,010 - hofC b0106 0.41 -1.6 115,021 + hofC b0106 0.41 -0.2 115,021 + hofC b0106 0.41 -1.9 115,077 - hofC b0106 0.46 +0.1 115,152 - hofC b0106 0.52 -0.3 115,152 - hofC b0106 0.52 -0.5 115,201 - hofC b0106 0.56 -0.7 115,203 - hofC b0106 0.57 -1.1 115,203 - hofC b0106 0.57 -1.1 115,273 + hofC b0106 0.62 -0.4 115,278 + hofC b0106 0.63 -2.1 115,278 + hofC b0106 0.63 -0.5 115,330 + hofC b0106 0.67 -0.6 115,367 + hofC b0106 0.70 +1.1 115,367 + hofC b0106 0.70 +0.4 115,379 + hofC b0106 0.71 -0.6 115,379 + hofC b0106 0.71 +0.4 115,409 + hofC b0106 0.74 +0.8 115,474 - hofC b0106 0.79 -0.3 115,507 + hofC b0106 0.82 -0.6 115,507 + hofC b0106 0.82 -1.1 115,515 - hofC b0106 0.83 +0.1 115,624 + +0.5 115,651 + +0.2 115,699 - -1.4 115,737 + -0.3 115,856 - hofB b0107 0.10 +0.2 115,856 - hofB b0107 0.10 -2.0 115,910 + hofB b0107 0.14 -0.8 115,911 - hofB b0107 0.14 +1.0 115,911 - hofB b0107 0.14 +0.6 115,941 + hofB b0107 0.16 -1.2 115,980 + hofB b0107 0.19 +0.4 116,058 + hofB b0107 0.25 -0.5 116,149 - hofB b0107 0.31 +0.1 116,335 + hofB b0107 0.45 +0.5 116,399 + hofB b0107 0.49 +0.1 116,430 - hofB b0107 0.52 +0.8 116,430 - hofB b0107 0.52 +1.3 116,471 - hofB b0107 0.55 -0.5
Or see this region's nucleotide sequence