Strain Fitness in Escherichia coli BW25113 around b1633

Experiment: Chloramphenicol 0.0025 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrsxG and rsxE are separated by 3 nucleotidesrsxE and nth overlap by 1 nucleotidesnth and tppB are separated by 610 nucleotides b1631: rsxG - electron transport complex protein RnfG (NCBI), at 1,708,228 to 1,708,848 rsxG b1632: rsxE - NADH-ubiquinone oxidoreductase (NCBI), at 1,708,852 to 1,709,547 rsxE b1633: nth - DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) (NCBI), at 1,709,547 to 1,710,182 nth b1634: tppB - putative tripeptide transporter permease (NCBI), at 1,710,793 to 1,712,295 tppB Position (kb) 1709 1710 1711Strain fitness (log2 ratio) -1 0 1 2 3 4at 1708.996 kb on - strand, within rsxEat 1709.004 kb on + strand, within rsxEat 1709.034 kb on - strand, within rsxEat 1709.096 kb on + strand, within rsxEat 1709.096 kb on + strand, within rsxEat 1709.106 kb on - strand, within rsxEat 1709.123 kb on + strand, within rsxEat 1709.128 kb on + strand, within rsxEat 1709.182 kb on + strand, within rsxEat 1709.254 kb on + strand, within rsxEat 1709.303 kb on - strand, within rsxEat 1709.320 kb on + strand, within rsxEat 1709.330 kb on - strand, within rsxEat 1709.330 kb on - strand, within rsxEat 1709.393 kb on + strand, within rsxEat 1709.445 kb on + strand, within rsxEat 1709.464 kb on - strand, within rsxEat 1709.517 kb on - strandat 1709.581 kb on + strandat 1709.581 kb on + strandat 1709.691 kb on - strand, within nthat 1709.760 kb on - strand, within nthat 1709.843 kb on + strand, within nthat 1709.859 kb on + strand, within nthat 1709.859 kb on + strand, within nthat 1709.876 kb on + strand, within nthat 1709.876 kb on + strand, within nthat 1710.159 kb on + strandat 1710.274 kb on - strandat 1710.551 kb on - strandat 1710.837 kb on - strandat 1710.838 kb on - strandat 1710.870 kb on - strandat 1711.174 kb on - strand, within tppB

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Per-strain Table

Position Strand Gene LocusTag Fraction Chloramphenicol 0.0025 mg/ml
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1,708,996 - rsxE b1632 0.21 +2.0
1,709,004 + rsxE b1632 0.22 +1.8
1,709,034 - rsxE b1632 0.26 +2.5
1,709,096 + rsxE b1632 0.35 +1.8
1,709,096 + rsxE b1632 0.35 +2.1
1,709,106 - rsxE b1632 0.36 +1.8
1,709,123 + rsxE b1632 0.39 +1.8
1,709,128 + rsxE b1632 0.40 +2.6
1,709,182 + rsxE b1632 0.47 +3.3
1,709,254 + rsxE b1632 0.58 +2.7
1,709,303 - rsxE b1632 0.65 +2.5
1,709,320 + rsxE b1632 0.67 +1.0
1,709,330 - rsxE b1632 0.69 +1.8
1,709,330 - rsxE b1632 0.69 +4.3
1,709,393 + rsxE b1632 0.78 +1.8
1,709,445 + rsxE b1632 0.85 +2.1
1,709,464 - rsxE b1632 0.88 +2.5
1,709,517 - +2.3
1,709,581 + +0.6
1,709,581 + -1.4
1,709,691 - nth b1633 0.23 +1.2
1,709,760 - nth b1633 0.33 +0.6
1,709,843 + nth b1633 0.47 +0.2
1,709,859 + nth b1633 0.49 -1.0
1,709,859 + nth b1633 0.49 +0.6
1,709,876 + nth b1633 0.52 +0.6
1,709,876 + nth b1633 0.52 +1.3
1,710,159 + -1.0
1,710,274 - +0.7
1,710,551 - +0.3
1,710,837 - +0.6
1,710,838 - +0.2
1,710,870 - -0.6
1,711,174 - tppB b1634 0.25 +1.5

Or see this region's nucleotide sequence