Experiment: Chloramphenicol 0.0025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potI and ybjO are separated by 59 nucleotides ybjO and rumB are separated by 40 nucleotides rumB and artJ are separated by 198 nucleotides
b0857: potI - putrescine transporter subunit: membrane component of ABC superfamily (NCBI), at 896,307 to 897,152
potI
b0858: ybjO - predicted inner membrane protein (NCBI), at 897,212 to 897,700
ybjO
b0859: rumB - 23S rRNA m(5)U747-methyltransferase (NCBI), at 897,741 to 898,868
rumB
b0860: artJ - arginine transporter subunit (NCBI), at 899,067 to 899,798
artJ
Position (kb) 897
898
899 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 897.041 kb on + strand, within potI at 897.053 kb on - strand, within potI at 897.084 kb on - strand at 897.084 kb on - strand at 897.371 kb on - strand, within ybjO at 897.402 kb on - strand, within ybjO at 897.426 kb on + strand, within ybjO at 897.702 kb on - strand at 897.724 kb on + strand at 897.744 kb on - strand at 897.780 kb on - strand at 897.815 kb on - strand at 897.815 kb on - strand at 897.894 kb on + strand, within rumB at 897.894 kb on + strand, within rumB at 897.929 kb on + strand, within rumB at 897.929 kb on + strand, within rumB at 897.953 kb on + strand, within rumB at 897.956 kb on - strand, within rumB at 897.960 kb on + strand, within rumB at 898.006 kb on - strand, within rumB at 898.006 kb on - strand, within rumB at 898.164 kb on - strand, within rumB at 898.193 kb on - strand, within rumB at 898.201 kb on - strand, within rumB at 898.201 kb on - strand, within rumB at 898.293 kb on + strand, within rumB at 898.331 kb on + strand, within rumB at 898.331 kb on + strand, within rumB at 898.455 kb on + strand, within rumB at 898.455 kb on + strand, within rumB at 898.468 kb on + strand, within rumB at 898.468 kb on - strand, within rumB at 898.482 kb on + strand, within rumB at 898.490 kb on - strand, within rumB at 898.490 kb on - strand, within rumB at 898.497 kb on + strand, within rumB at 898.498 kb on + strand, within rumB at 898.549 kb on + strand, within rumB at 898.549 kb on + strand, within rumB at 898.549 kb on + strand, within rumB at 898.700 kb on + strand, within rumB at 898.700 kb on + strand, within rumB at 898.737 kb on + strand, within rumB at 898.827 kb on + strand at 898.827 kb on + strand at 898.946 kb on - strand at 898.946 kb on - strand at 899.181 kb on + strand, within artJ at 899.189 kb on - strand, within artJ at 899.189 kb on - strand, within artJ at 899.335 kb on + strand, within artJ at 899.487 kb on - strand, within artJ at 899.497 kb on + strand, within artJ at 899.497 kb on + strand, within artJ at 899.515 kb on - strand, within artJ at 899.520 kb on - strand, within artJ at 899.522 kb on + strand, within artJ at 899.522 kb on + strand, within artJ at 899.684 kb on + strand, within artJ at 899.846 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0025 mg/ml remove 897,041 + potI b0857 0.87 +0.3 897,053 - potI b0857 0.88 +1.8 897,084 - -1.0 897,084 - +0.3 897,371 - ybjO b0858 0.33 -0.2 897,402 - ybjO b0858 0.39 +1.7 897,426 + ybjO b0858 0.44 +1.1 897,702 - +0.7 897,724 + +0.6 897,744 - +0.5 897,780 - -0.0 897,815 - -1.2 897,815 - -1.0 897,894 + rumB b0859 0.14 -0.5 897,894 + rumB b0859 0.14 -1.2 897,929 + rumB b0859 0.17 -0.2 897,929 + rumB b0859 0.17 -1.2 897,953 + rumB b0859 0.19 -0.8 897,956 - rumB b0859 0.19 -0.6 897,960 + rumB b0859 0.19 -0.4 898,006 - rumB b0859 0.23 -0.7 898,006 - rumB b0859 0.23 +0.4 898,164 - rumB b0859 0.38 -0.9 898,193 - rumB b0859 0.40 -1.2 898,201 - rumB b0859 0.41 -1.6 898,201 - rumB b0859 0.41 -0.6 898,293 + rumB b0859 0.49 -0.8 898,331 + rumB b0859 0.52 -0.2 898,331 + rumB b0859 0.52 -2.1 898,455 + rumB b0859 0.63 -0.7 898,455 + rumB b0859 0.63 -0.2 898,468 + rumB b0859 0.64 +0.0 898,468 - rumB b0859 0.64 +0.3 898,482 + rumB b0859 0.66 -2.0 898,490 - rumB b0859 0.66 +0.4 898,490 - rumB b0859 0.66 -0.2 898,497 + rumB b0859 0.67 +0.3 898,498 + rumB b0859 0.67 +0.8 898,549 + rumB b0859 0.72 -0.0 898,549 + rumB b0859 0.72 -0.2 898,549 + rumB b0859 0.72 +0.5 898,700 + rumB b0859 0.85 -1.5 898,700 + rumB b0859 0.85 -1.0 898,737 + rumB b0859 0.88 -0.3 898,827 + -1.3 898,827 + -0.2 898,946 - +3.6 898,946 - -0.3 899,181 + artJ b0860 0.16 -0.6 899,189 - artJ b0860 0.17 -0.7 899,189 - artJ b0860 0.17 +0.8 899,335 + artJ b0860 0.37 -1.5 899,487 - artJ b0860 0.57 +0.3 899,497 + artJ b0860 0.59 +0.6 899,497 + artJ b0860 0.59 -0.1 899,515 - artJ b0860 0.61 +2.8 899,520 - artJ b0860 0.62 +0.6 899,522 + artJ b0860 0.62 +1.0 899,522 + artJ b0860 0.62 +0.4 899,684 + artJ b0860 0.84 -0.2 899,846 - +1.8
Or see this region's nucleotide sequence