Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_659 and PfGW456L13_660 are separated by 10 nucleotides PfGW456L13_660 and PfGW456L13_661 are separated by 83 nucleotides PfGW456L13_661 and PfGW456L13_662 are separated by 221 nucleotides PfGW456L13_662 and PfGW456L13_663 are separated by 60 nucleotides
PfGW456L13_659 - Glycyl-tRNA synthetase beta chain (EC 6.1.1.14), at 748,506 to 750,560
_659
PfGW456L13_660 - D-glycero-D-manno-heptose 1,7-bisphosphate phosphatase (EC 3.1.1.-); Histidinol-phosphatase (EC 3.1.3.15), at 750,571 to 751,104
_660
PfGW456L13_661 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 751,188 to 751,967
_661
PfGW456L13_662 - Two-component response regulator, at 752,189 to 752,431
_662
PfGW456L13_663 - DNA gyrase subunit B (EC 5.99.1.3), at 752,492 to 754,870
_663
Position (kb)
751
752 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 751.092 kb on + strand at 751.102 kb on + strand at 751.150 kb on + strand at 751.177 kb on + strand at 751.177 kb on + strand at 752.115 kb on - strand at 752.127 kb on + strand at 752.127 kb on + strand at 752.182 kb on - strand at 752.208 kb on - strand at 752.265 kb on - strand, within PfGW456L13_662 at 752.265 kb on - strand, within PfGW456L13_662 at 752.270 kb on + strand, within PfGW456L13_662 at 752.330 kb on + strand, within PfGW456L13_662 at 752.330 kb on + strand, within PfGW456L13_662 at 752.330 kb on + strand, within PfGW456L13_662 at 752.330 kb on + strand, within PfGW456L13_662 at 752.331 kb on - strand, within PfGW456L13_662 at 752.331 kb on - strand, within PfGW456L13_662 at 752.331 kb on - strand, within PfGW456L13_662 at 752.390 kb on + strand, within PfGW456L13_662 at 752.391 kb on - strand, within PfGW456L13_662 at 752.429 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 751,092 + -0.4 751,102 + +0.7 751,150 + +2.3 751,177 + +1.6 751,177 + +2.6 752,115 - +1.3 752,127 + +0.6 752,127 + -1.9 752,182 - +1.1 752,208 - -0.3 752,265 - PfGW456L13_662 0.31 -0.2 752,265 - PfGW456L13_662 0.31 -3.5 752,270 + PfGW456L13_662 0.33 -0.4 752,330 + PfGW456L13_662 0.58 -2.6 752,330 + PfGW456L13_662 0.58 -0.1 752,330 + PfGW456L13_662 0.58 -1.5 752,330 + PfGW456L13_662 0.58 -2.2 752,331 - PfGW456L13_662 0.58 -3.2 752,331 - PfGW456L13_662 0.58 -2.9 752,331 - PfGW456L13_662 0.58 -2.3 752,390 + PfGW456L13_662 0.83 +1.2 752,391 - PfGW456L13_662 0.83 +0.2 752,429 + -2.2
Or see this region's nucleotide sequence