Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_60 and PfGW456L13_61 are separated by 187 nucleotides PfGW456L13_61 and PfGW456L13_62 are separated by 78 nucleotides PfGW456L13_62 and PfGW456L13_63 are separated by 148 nucleotides PfGW456L13_63 and PfGW456L13_64 are separated by 618 nucleotides
PfGW456L13_60 - membrane protein, MarC family, at 72,300 to 72,896
_60
PfGW456L13_61 - hypothetical protein, at 73,084 to 73,407
_61
PfGW456L13_62 - hypothetical protein, at 73,486 to 73,599
_62
PfGW456L13_63 - hypothetical protein, at 73,748 to 74,167
_63
PfGW456L13_64 - Cobalamin biosynthesis protein CbiG / Cobalt-precorrin-3b C17-methyltransferase, at 74,786 to 76,492
_64
Position (kb) 73
74
75 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 73.028 kb on - strand at 73.061 kb on + strand at 73.061 kb on + strand at 73.061 kb on + strand at 73.061 kb on + strand at 73.062 kb on - strand at 73.062 kb on - strand at 73.062 kb on - strand at 73.062 kb on - strand at 73.156 kb on + strand, within PfGW456L13_61 at 73.157 kb on - strand, within PfGW456L13_61 at 73.260 kb on - strand, within PfGW456L13_61 at 73.260 kb on - strand, within PfGW456L13_61 at 73.435 kb on + strand at 73.436 kb on - strand at 73.531 kb on + strand, within PfGW456L13_62 at 73.598 kb on - strand at 73.655 kb on - strand at 73.693 kb on - strand at 73.804 kb on - strand, within PfGW456L13_63 at 74.083 kb on + strand, within PfGW456L13_63 at 74.084 kb on - strand, within PfGW456L13_63 at 74.084 kb on - strand, within PfGW456L13_63 at 74.084 kb on - strand, within PfGW456L13_63 at 74.156 kb on - strand at 74.174 kb on - strand at 74.183 kb on + strand at 74.188 kb on + strand at 74.308 kb on - strand at 74.370 kb on - strand at 74.390 kb on + strand at 74.405 kb on + strand at 74.406 kb on - strand at 74.429 kb on - strand at 74.463 kb on - strand at 74.578 kb on + strand at 74.578 kb on + strand at 74.580 kb on + strand at 74.581 kb on - strand at 74.654 kb on + strand at 74.661 kb on + strand at 74.661 kb on + strand at 74.695 kb on - strand at 74.743 kb on + strand at 74.744 kb on - strand at 74.744 kb on - strand at 74.767 kb on - strand at 74.777 kb on + strand at 74.788 kb on - strand at 74.789 kb on + strand at 74.789 kb on + strand at 74.790 kb on - strand at 74.811 kb on + strand at 74.812 kb on - strand at 74.826 kb on + strand at 74.836 kb on + strand at 74.836 kb on + strand at 74.837 kb on - strand at 74.885 kb on + strand at 74.886 kb on - strand at 74.886 kb on - strand at 74.886 kb on - strand at 75.032 kb on + strand, within PfGW456L13_64 at 75.033 kb on - strand, within PfGW456L13_64 at 75.033 kb on - strand, within PfGW456L13_64 at 75.066 kb on + strand, within PfGW456L13_64 at 75.066 kb on + strand, within PfGW456L13_64 at 75.067 kb on - strand, within PfGW456L13_64 at 75.067 kb on - strand, within PfGW456L13_64 at 75.067 kb on - strand, within PfGW456L13_64
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 73,028 - +3.2 73,061 + -0.9 73,061 + -3.6 73,061 + +0.3 73,061 + +1.7 73,062 - -2.5 73,062 - +0.7 73,062 - -1.5 73,062 - +2.7 73,156 + PfGW456L13_61 0.22 +1.4 73,157 - PfGW456L13_61 0.23 -0.4 73,260 - PfGW456L13_61 0.54 +1.3 73,260 - PfGW456L13_61 0.54 +1.9 73,435 + -0.3 73,436 - +2.0 73,531 + PfGW456L13_62 0.39 -0.0 73,598 - -1.9 73,655 - -2.9 73,693 - -0.1 73,804 - PfGW456L13_63 0.13 -1.2 74,083 + PfGW456L13_63 0.80 -1.2 74,084 - PfGW456L13_63 0.80 -1.5 74,084 - PfGW456L13_63 0.80 -0.9 74,084 - PfGW456L13_63 0.80 -3.5 74,156 - -3.1 74,174 - +3.3 74,183 + +2.3 74,188 + +0.6 74,308 - -0.5 74,370 - -0.4 74,390 + -2.9 74,405 + +0.9 74,406 - -2.2 74,429 - -3.8 74,463 - -0.4 74,578 + -0.1 74,578 + +0.9 74,580 + -2.0 74,581 - -0.2 74,654 + +1.0 74,661 + -1.3 74,661 + +1.3 74,695 - +1.9 74,743 + -1.0 74,744 - -3.2 74,744 - +1.7 74,767 - -0.4 74,777 + -1.3 74,788 - +0.9 74,789 + +2.1 74,789 + +1.1 74,790 - -0.3 74,811 + +0.1 74,812 - +0.2 74,826 + +0.9 74,836 + +0.3 74,836 + -1.6 74,837 - -1.1 74,885 + -3.1 74,886 - -2.8 74,886 - +0.5 74,886 - -0.8 75,032 + PfGW456L13_64 0.14 +1.0 75,033 - PfGW456L13_64 0.14 +2.6 75,033 - PfGW456L13_64 0.14 +2.5 75,066 + PfGW456L13_64 0.16 +0.3 75,066 + PfGW456L13_64 0.16 +0.8 75,067 - PfGW456L13_64 0.16 -4.1 75,067 - PfGW456L13_64 0.16 -0.8 75,067 - PfGW456L13_64 0.16 +0.3
Or see this region's nucleotide sequence