Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2181 and PfGW456L13_2182 are separated by 360 nucleotides PfGW456L13_2182 and PfGW456L13_2183 are separated by 426 nucleotides
PfGW456L13_2181 - Integration host factor beta subunit, at 2,391,409 to 2,391,705
_2181
PfGW456L13_2182 - regulator of length of O-antigen component of lipopolysaccharide chains, at 2,392,066 to 2,393,136
_2182
PfGW456L13_2183 - WzxE protein, at 2,393,563 to 2,394,822
_2183
Position (kb)
2392
2393
2394 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2391.111 kb on - strand at 2391.238 kb on + strand at 2391.239 kb on - strand at 2391.239 kb on - strand at 2391.239 kb on - strand at 2391.239 kb on - strand at 2391.318 kb on + strand at 2391.318 kb on + strand at 2391.319 kb on - strand at 2391.319 kb on - strand at 2391.325 kb on + strand at 2391.572 kb on - strand, within PfGW456L13_2181 at 2391.572 kb on - strand, within PfGW456L13_2181 at 2391.763 kb on + strand at 2391.764 kb on - strand at 2391.764 kb on - strand at 2391.766 kb on + strand at 2391.766 kb on + strand at 2391.767 kb on - strand at 2391.842 kb on + strand at 2391.861 kb on + strand at 2391.862 kb on - strand at 2391.862 kb on - strand at 2391.863 kb on + strand at 2391.863 kb on + strand at 2391.863 kb on + strand at 2391.863 kb on + strand at 2391.863 kb on + strand at 2391.864 kb on - strand at 2391.864 kb on - strand at 2392.042 kb on + strand at 2392.050 kb on - strand at 2392.106 kb on + strand at 2392.106 kb on + strand at 2392.194 kb on - strand, within PfGW456L13_2182 at 2392.219 kb on + strand, within PfGW456L13_2182 at 2392.219 kb on + strand, within PfGW456L13_2182 at 2392.272 kb on + strand, within PfGW456L13_2182 at 2392.335 kb on - strand, within PfGW456L13_2182 at 2392.467 kb on + strand, within PfGW456L13_2182 at 2392.467 kb on + strand, within PfGW456L13_2182 at 2392.468 kb on - strand, within PfGW456L13_2182 at 2392.468 kb on - strand, within PfGW456L13_2182 at 2392.469 kb on + strand, within PfGW456L13_2182 at 2392.469 kb on + strand, within PfGW456L13_2182 at 2392.470 kb on - strand, within PfGW456L13_2182 at 2392.470 kb on - strand, within PfGW456L13_2182 at 2392.558 kb on + strand, within PfGW456L13_2182 at 2392.558 kb on + strand, within PfGW456L13_2182 at 2392.559 kb on - strand, within PfGW456L13_2182 at 2392.559 kb on - strand, within PfGW456L13_2182 at 2392.707 kb on - strand, within PfGW456L13_2182 at 2392.707 kb on - strand, within PfGW456L13_2182 at 2392.728 kb on + strand, within PfGW456L13_2182 at 2392.790 kb on + strand, within PfGW456L13_2182 at 2392.813 kb on + strand, within PfGW456L13_2182 at 2392.813 kb on + strand, within PfGW456L13_2182 at 2392.813 kb on + strand, within PfGW456L13_2182 at 2392.813 kb on + strand, within PfGW456L13_2182 at 2392.814 kb on - strand, within PfGW456L13_2182 at 2392.814 kb on - strand, within PfGW456L13_2182 at 2392.814 kb on - strand, within PfGW456L13_2182 at 2392.815 kb on + strand, within PfGW456L13_2182 at 2392.964 kb on - strand, within PfGW456L13_2182 at 2393.083 kb on + strand at 2393.130 kb on + strand at 2393.131 kb on - strand at 2393.244 kb on + strand at 2393.245 kb on - strand at 2393.245 kb on - strand at 2393.245 kb on - strand at 2393.245 kb on - strand at 2393.259 kb on - strand at 2393.334 kb on + strand at 2393.334 kb on + strand at 2393.344 kb on + strand at 2393.359 kb on + strand at 2393.360 kb on - strand at 2393.363 kb on + strand at 2393.363 kb on + strand at 2393.375 kb on + strand at 2393.376 kb on - strand at 2393.376 kb on - strand at 2393.380 kb on - strand at 2393.380 kb on - strand at 2393.410 kb on + strand at 2393.417 kb on + strand at 2393.421 kb on - strand at 2393.471 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 2,391,111 - +1.7 2,391,238 + +2.1 2,391,239 - -0.9 2,391,239 - -1.1 2,391,239 - +1.0 2,391,239 - -0.1 2,391,318 + -2.2 2,391,318 + +2.5 2,391,319 - -0.1 2,391,319 - -1.9 2,391,325 + +1.2 2,391,572 - PfGW456L13_2181 0.55 -1.1 2,391,572 - PfGW456L13_2181 0.55 -2.0 2,391,763 + -1.7 2,391,764 - -3.2 2,391,764 - -3.0 2,391,766 + +1.9 2,391,766 + +0.8 2,391,767 - -0.7 2,391,842 + +1.8 2,391,861 + +0.2 2,391,862 - -2.2 2,391,862 - -2.8 2,391,863 + +1.4 2,391,863 + +2.0 2,391,863 + +3.7 2,391,863 + +1.7 2,391,863 + +2.9 2,391,864 - -4.0 2,391,864 - -1.4 2,392,042 + -2.8 2,392,050 - +0.6 2,392,106 + -2.6 2,392,106 + -3.4 2,392,194 - PfGW456L13_2182 0.12 -1.9 2,392,219 + PfGW456L13_2182 0.14 -3.0 2,392,219 + PfGW456L13_2182 0.14 +0.4 2,392,272 + PfGW456L13_2182 0.19 -4.3 2,392,335 - PfGW456L13_2182 0.25 -2.8 2,392,467 + PfGW456L13_2182 0.37 -0.1 2,392,467 + PfGW456L13_2182 0.37 -0.8 2,392,468 - PfGW456L13_2182 0.38 -3.2 2,392,468 - PfGW456L13_2182 0.38 -2.2 2,392,469 + PfGW456L13_2182 0.38 -3.3 2,392,469 + PfGW456L13_2182 0.38 -3.7 2,392,470 - PfGW456L13_2182 0.38 -4.2 2,392,470 - PfGW456L13_2182 0.38 -1.3 2,392,558 + PfGW456L13_2182 0.46 -2.8 2,392,558 + PfGW456L13_2182 0.46 -3.5 2,392,559 - PfGW456L13_2182 0.46 -0.7 2,392,559 - PfGW456L13_2182 0.46 -2.1 2,392,707 - PfGW456L13_2182 0.60 -3.1 2,392,707 - PfGW456L13_2182 0.60 -0.7 2,392,728 + PfGW456L13_2182 0.62 -2.5 2,392,790 + PfGW456L13_2182 0.68 -1.9 2,392,813 + PfGW456L13_2182 0.70 -2.4 2,392,813 + PfGW456L13_2182 0.70 -2.8 2,392,813 + PfGW456L13_2182 0.70 -3.0 2,392,813 + PfGW456L13_2182 0.70 -1.2 2,392,814 - PfGW456L13_2182 0.70 -2.1 2,392,814 - PfGW456L13_2182 0.70 -1.4 2,392,814 - PfGW456L13_2182 0.70 -3.4 2,392,815 + PfGW456L13_2182 0.70 -3.5 2,392,964 - PfGW456L13_2182 0.84 -1.9 2,393,083 + -3.7 2,393,130 + -1.4 2,393,131 - -0.8 2,393,244 + +0.3 2,393,245 - -2.7 2,393,245 - -0.7 2,393,245 - -0.8 2,393,245 - -2.9 2,393,259 - -4.0 2,393,334 + +2.5 2,393,334 + +2.6 2,393,344 + +0.0 2,393,359 + -1.1 2,393,360 - -4.2 2,393,363 + +3.4 2,393,363 + -0.7 2,393,375 + -2.9 2,393,376 - -1.7 2,393,376 - -3.0 2,393,380 - -1.2 2,393,380 - -3.9 2,393,410 + +0.8 2,393,417 + -1.1 2,393,421 - -3.5 2,393,471 - -3.2
Or see this region's nucleotide sequence