Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2009 and PfGW456L13_2010 are separated by 233 nucleotides PfGW456L13_2010 and PfGW456L13_2011 are separated by 120 nucleotides PfGW456L13_2011 and PfGW456L13_2012 overlap by 4 nucleotides
PfGW456L13_2009 - 4'-phosphopantetheinyl transferase entD (EC 2.7.8.-); Holo-[acyl-carrier protein] synthase, alternative (EC 2.7.8.7), at 2,213,562 to 2,214,287
_2009
PfGW456L13_2010 - outer membrane protein H1, at 2,214,521 to 2,215,123
_2010
PfGW456L13_2011 - Transcriptional regulatory protein PhoP, at 2,215,244 to 2,215,921
_2011
PfGW456L13_2012 - Sensor protein PhoQ (EC 2.7.13.3), at 2,215,918 to 2,217,264
_2012
Position (kb)
2214
2215
2216 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2214.287 kb on + strand at 2214.288 kb on - strand at 2214.288 kb on - strand at 2214.323 kb on - strand at 2214.348 kb on - strand at 2214.610 kb on + strand, within PfGW456L13_2010 at 2214.610 kb on + strand, within PfGW456L13_2010 at 2214.611 kb on - strand, within PfGW456L13_2010 at 2214.611 kb on - strand, within PfGW456L13_2010 at 2214.611 kb on - strand, within PfGW456L13_2010 at 2214.637 kb on + strand, within PfGW456L13_2010 at 2214.752 kb on + strand, within PfGW456L13_2010 at 2214.752 kb on + strand, within PfGW456L13_2010 at 2214.752 kb on + strand, within PfGW456L13_2010 at 2214.752 kb on + strand, within PfGW456L13_2010 at 2214.753 kb on - strand, within PfGW456L13_2010 at 2214.761 kb on + strand, within PfGW456L13_2010 at 2214.761 kb on + strand, within PfGW456L13_2010 at 2214.761 kb on + strand, within PfGW456L13_2010 at 2214.761 kb on + strand, within PfGW456L13_2010 at 2214.762 kb on - strand, within PfGW456L13_2010 at 2214.762 kb on - strand, within PfGW456L13_2010 at 2214.824 kb on + strand, within PfGW456L13_2010 at 2214.824 kb on + strand, within PfGW456L13_2010 at 2214.824 kb on + strand, within PfGW456L13_2010 at 2214.825 kb on - strand, within PfGW456L13_2010 at 2214.825 kb on - strand, within PfGW456L13_2010 at 2214.836 kb on + strand, within PfGW456L13_2010 at 2215.004 kb on + strand, within PfGW456L13_2010 at 2215.011 kb on - strand, within PfGW456L13_2010 at 2215.097 kb on + strand at 2215.097 kb on + strand at 2215.097 kb on + strand at 2215.097 kb on + strand at 2215.097 kb on + strand at 2215.097 kb on + strand at 2215.097 kb on + strand at 2215.097 kb on + strand at 2215.098 kb on - strand at 2215.098 kb on - strand at 2215.098 kb on - strand at 2215.112 kb on + strand at 2215.186 kb on + strand at 2215.186 kb on + strand at 2215.186 kb on + strand at 2215.187 kb on - strand at 2215.212 kb on + strand at 2215.213 kb on - strand at 2215.535 kb on + strand, within PfGW456L13_2011 at 2215.535 kb on + strand, within PfGW456L13_2011 at 2215.535 kb on + strand, within PfGW456L13_2011 at 2215.536 kb on - strand, within PfGW456L13_2011 at 2215.536 kb on - strand, within PfGW456L13_2011 at 2215.700 kb on + strand, within PfGW456L13_2011 at 2215.701 kb on - strand, within PfGW456L13_2011 at 2215.701 kb on - strand, within PfGW456L13_2011 at 2215.721 kb on + strand, within PfGW456L13_2011 at 2215.721 kb on + strand, within PfGW456L13_2011 at 2215.722 kb on - strand, within PfGW456L13_2011 at 2215.722 kb on - strand, within PfGW456L13_2011 at 2215.937 kb on + strand at 2215.937 kb on + strand at 2215.937 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 2,214,287 + -3.5 2,214,288 - -2.0 2,214,288 - -0.1 2,214,323 - -2.8 2,214,348 - +0.0 2,214,610 + PfGW456L13_2010 0.15 -3.6 2,214,610 + PfGW456L13_2010 0.15 -2.9 2,214,611 - PfGW456L13_2010 0.15 -2.6 2,214,611 - PfGW456L13_2010 0.15 -4.1 2,214,611 - PfGW456L13_2010 0.15 -3.1 2,214,637 + PfGW456L13_2010 0.19 -1.0 2,214,752 + PfGW456L13_2010 0.38 -2.5 2,214,752 + PfGW456L13_2010 0.38 -3.4 2,214,752 + PfGW456L13_2010 0.38 -4.2 2,214,752 + PfGW456L13_2010 0.38 -5.6 2,214,753 - PfGW456L13_2010 0.38 -4.5 2,214,761 + PfGW456L13_2010 0.40 -2.9 2,214,761 + PfGW456L13_2010 0.40 -4.2 2,214,761 + PfGW456L13_2010 0.40 -4.7 2,214,761 + PfGW456L13_2010 0.40 -4.4 2,214,762 - PfGW456L13_2010 0.40 -4.4 2,214,762 - PfGW456L13_2010 0.40 -2.3 2,214,824 + PfGW456L13_2010 0.50 -4.1 2,214,824 + PfGW456L13_2010 0.50 -2.6 2,214,824 + PfGW456L13_2010 0.50 -4.0 2,214,825 - PfGW456L13_2010 0.50 -3.3 2,214,825 - PfGW456L13_2010 0.50 -3.5 2,214,836 + PfGW456L13_2010 0.52 -1.3 2,215,004 + PfGW456L13_2010 0.80 -2.1 2,215,011 - PfGW456L13_2010 0.81 -4.1 2,215,097 + -3.6 2,215,097 + -2.8 2,215,097 + -4.1 2,215,097 + -3.0 2,215,097 + -2.0 2,215,097 + -3.7 2,215,097 + -3.9 2,215,097 + -3.9 2,215,098 - -1.3 2,215,098 - -3.0 2,215,098 - -1.3 2,215,112 + -3.5 2,215,186 + -2.2 2,215,186 + +1.6 2,215,186 + -0.8 2,215,187 - -3.2 2,215,212 + +1.6 2,215,213 - -1.6 2,215,535 + PfGW456L13_2011 0.43 -3.8 2,215,535 + PfGW456L13_2011 0.43 -4.8 2,215,535 + PfGW456L13_2011 0.43 -3.8 2,215,536 - PfGW456L13_2011 0.43 -3.9 2,215,536 - PfGW456L13_2011 0.43 -2.6 2,215,700 + PfGW456L13_2011 0.67 -1.6 2,215,701 - PfGW456L13_2011 0.67 -3.7 2,215,701 - PfGW456L13_2011 0.67 -3.1 2,215,721 + PfGW456L13_2011 0.70 -3.8 2,215,721 + PfGW456L13_2011 0.70 -3.6 2,215,722 - PfGW456L13_2011 0.71 -4.2 2,215,722 - PfGW456L13_2011 0.71 -4.0 2,215,937 + +0.4 2,215,937 + +0.6 2,215,937 + -3.6
Or see this region's nucleotide sequence