Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_1477 and PfGW456L13_1478 are separated by 37 nucleotides PfGW456L13_1478 and PfGW456L13_1479 overlap by 8 nucleotides PfGW456L13_1479 and PfGW456L13_1480 are separated by 168 nucleotides
PfGW456L13_1477 - Isoleucyl-tRNA synthetase (EC 6.1.1.5), at 1,629,573 to 1,632,404
_1477
PfGW456L13_1478 - Lipoprotein signal peptidase (EC 3.4.23.36), at 1,632,442 to 1,632,909
_1478
PfGW456L13_1479 - FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8), at 1,632,902 to 1,633,354
_1479
PfGW456L13_1480 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2), at 1,633,523 to 1,634,470
_1480
Position (kb)
1632
1633
1634 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1632.867 kb on + strand at 1632.915 kb on + strand at 1632.915 kb on + strand at 1632.915 kb on + strand at 1633.231 kb on + strand, within PfGW456L13_1479 at 1633.326 kb on + strand at 1633.326 kb on + strand at 1633.326 kb on + strand at 1633.326 kb on + strand at 1633.352 kb on + strand at 1633.352 kb on + strand at 1633.352 kb on + strand at 1633.417 kb on + strand at 1633.431 kb on + strand at 1633.431 kb on + strand at 1633.437 kb on + strand at 1633.437 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 1,632,867 + -3.3 1,632,915 + -3.1 1,632,915 + -1.9 1,632,915 + -2.0 1,633,231 + PfGW456L13_1479 0.73 -3.9 1,633,326 + -1.5 1,633,326 + -3.1 1,633,326 + -3.0 1,633,326 + -2.2 1,633,352 + +1.1 1,633,352 + -0.6 1,633,352 + +0.3 1,633,417 + -2.0 1,633,431 + +0.6 1,633,431 + +0.5 1,633,437 + +0.6 1,633,437 + +1.1
Or see this region's nucleotide sequence