Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_1311 and PfGW456L13_1312 are separated by 127 nucleotides PfGW456L13_1312 and PfGW456L13_1313 overlap by 4 nucleotides PfGW456L13_1313 and PfGW456L13_1314 are separated by 47 nucleotides PfGW456L13_1314 and PfGW456L13_1315 are separated by 16 nucleotides
PfGW456L13_1311 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 1,452,307 to 1,452,771
_1311
PfGW456L13_1312 - Ribonucleotide reductase transcriptional regulator NrdR, at 1,452,899 to 1,453,363
_1312
PfGW456L13_1313 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 1,453,360 to 1,454,493
_1313
PfGW456L13_1314 - Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9), at 1,454,541 to 1,455,206
_1314
PfGW456L13_1315 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25), at 1,455,223 to 1,456,314
_1315
Position (kb)
1453
1454
1455 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1452.360 kb on + strand, within PfGW456L13_1311 at 1452.534 kb on + strand, within PfGW456L13_1311 at 1452.763 kb on + strand at 1452.764 kb on - strand at 1452.783 kb on + strand at 1452.822 kb on + strand at 1452.822 kb on + strand at 1452.822 kb on + strand at 1452.822 kb on + strand at 1452.823 kb on - strand at 1452.823 kb on - strand at 1452.836 kb on + strand at 1452.837 kb on - strand at 1452.837 kb on - strand at 1452.864 kb on + strand at 1453.268 kb on + strand, within PfGW456L13_1312 at 1453.268 kb on + strand, within PfGW456L13_1312 at 1453.268 kb on + strand, within PfGW456L13_1312 at 1453.268 kb on + strand, within PfGW456L13_1312 at 1453.268 kb on + strand, within PfGW456L13_1312 at 1453.289 kb on + strand, within PfGW456L13_1312 at 1454.490 kb on - strand at 1454.491 kb on + strand at 1454.508 kb on + strand at 1454.508 kb on + strand at 1454.508 kb on + strand at 1455.234 kb on + strand at 1455.235 kb on - strand at 1455.235 kb on - strand at 1455.235 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 1,452,360 + PfGW456L13_1311 0.11 -1.1 1,452,534 + PfGW456L13_1311 0.49 +1.1 1,452,763 + +0.8 1,452,764 - -0.2 1,452,783 + +1.3 1,452,822 + -2.7 1,452,822 + +1.2 1,452,822 + -1.3 1,452,822 + -0.6 1,452,823 - -0.2 1,452,823 - +1.2 1,452,836 + -0.8 1,452,837 - +0.4 1,452,837 - +0.8 1,452,864 + -3.0 1,453,268 + PfGW456L13_1312 0.79 -0.4 1,453,268 + PfGW456L13_1312 0.79 -0.6 1,453,268 + PfGW456L13_1312 0.79 -0.9 1,453,268 + PfGW456L13_1312 0.79 -0.6 1,453,268 + PfGW456L13_1312 0.79 -2.5 1,453,289 + PfGW456L13_1312 0.84 +0.4 1,454,490 - -1.9 1,454,491 + -2.5 1,454,508 + +0.2 1,454,508 + -0.5 1,454,508 + -1.6 1,455,234 + -3.2 1,455,235 - +1.3 1,455,235 - -1.2 1,455,235 - +0.6
Or see this region's nucleotide sequence