Experiment: Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygdE and ygdD overlap by 8 nucleotides ygdD and gcvA are separated by 18 nucleotides
b2806: ygdE - predicted methyltransferase (NCBI), at 2,938,165 to 2,939,265
ygdE
b2807: ygdD - conserved inner membrane protein (NCBI), at 2,939,258 to 2,939,653
ygdD
b2808: gcvA - DNA-binding transcriptional dual regulator (NCBI), at 2,939,672 to 2,940,589
gcvA
Position (kb)
2939
2940 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2938.353 kb on - strand, within ygdE at 2938.353 kb on - strand, within ygdE at 2938.398 kb on - strand, within ygdE at 2938.443 kb on + strand, within ygdE at 2938.443 kb on - strand, within ygdE at 2938.553 kb on + strand, within ygdE at 2938.561 kb on - strand, within ygdE at 2938.561 kb on - strand, within ygdE at 2938.587 kb on + strand, within ygdE at 2938.610 kb on + strand, within ygdE at 2938.698 kb on + strand, within ygdE at 2938.711 kb on - strand, within ygdE at 2938.711 kb on - strand, within ygdE at 2938.750 kb on - strand, within ygdE at 2938.758 kb on + strand, within ygdE at 2938.780 kb on - strand, within ygdE at 2938.780 kb on - strand, within ygdE at 2938.795 kb on - strand, within ygdE at 2938.795 kb on - strand, within ygdE at 2938.810 kb on + strand, within ygdE at 2938.810 kb on + strand, within ygdE at 2938.868 kb on - strand, within ygdE at 2938.883 kb on - strand, within ygdE at 2938.969 kb on + strand, within ygdE at 2938.974 kb on + strand, within ygdE at 2938.974 kb on + strand, within ygdE at 2938.986 kb on + strand, within ygdE at 2939.038 kb on - strand, within ygdE at 2939.298 kb on - strand, within ygdD at 2939.347 kb on + strand, within ygdD at 2939.405 kb on + strand, within ygdD at 2939.442 kb on - strand, within ygdD at 2939.465 kb on - strand, within ygdD at 2939.470 kb on + strand, within ygdD at 2939.470 kb on + strand, within ygdD at 2939.545 kb on + strand, within ygdD at 2939.553 kb on - strand, within ygdD at 2939.648 kb on + strand at 2939.648 kb on + strand at 2939.655 kb on + strand at 2939.663 kb on - strand at 2939.663 kb on - strand at 2939.778 kb on - strand, within gcvA at 2939.778 kb on - strand, within gcvA at 2939.804 kb on + strand, within gcvA at 2939.912 kb on - strand, within gcvA at 2939.914 kb on - strand, within gcvA at 2939.982 kb on - strand, within gcvA at 2939.982 kb on - strand, within gcvA at 2939.985 kb on - strand, within gcvA at 2939.987 kb on - strand, within gcvA at 2940.001 kb on + strand, within gcvA at 2940.068 kb on + strand, within gcvA at 2940.171 kb on - strand, within gcvA at 2940.171 kb on - strand, within gcvA at 2940.289 kb on + strand, within gcvA at 2940.331 kb on + strand, within gcvA at 2940.399 kb on - strand, within gcvA at 2940.417 kb on - strand, within gcvA at 2940.477 kb on - strand, within gcvA at 2940.522 kb on + strand at 2940.587 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml remove 2,938,353 - ygdE b2806 0.17 -0.9 2,938,353 - ygdE b2806 0.17 +0.0 2,938,398 - ygdE b2806 0.21 -0.1 2,938,443 + ygdE b2806 0.25 -0.1 2,938,443 - ygdE b2806 0.25 -1.1 2,938,553 + ygdE b2806 0.35 -1.1 2,938,561 - ygdE b2806 0.36 -0.7 2,938,561 - ygdE b2806 0.36 -1.5 2,938,587 + ygdE b2806 0.38 -1.0 2,938,610 + ygdE b2806 0.40 +0.3 2,938,698 + ygdE b2806 0.48 -1.5 2,938,711 - ygdE b2806 0.50 -1.6 2,938,711 - ygdE b2806 0.50 -0.7 2,938,750 - ygdE b2806 0.53 -2.1 2,938,758 + ygdE b2806 0.54 -0.9 2,938,780 - ygdE b2806 0.56 +0.6 2,938,780 - ygdE b2806 0.56 -0.5 2,938,795 - ygdE b2806 0.57 -0.3 2,938,795 - ygdE b2806 0.57 -1.1 2,938,810 + ygdE b2806 0.59 +0.9 2,938,810 + ygdE b2806 0.59 +0.2 2,938,868 - ygdE b2806 0.64 -0.7 2,938,883 - ygdE b2806 0.65 -0.4 2,938,969 + ygdE b2806 0.73 -2.1 2,938,974 + ygdE b2806 0.73 -1.5 2,938,974 + ygdE b2806 0.73 -2.3 2,938,986 + ygdE b2806 0.75 -1.6 2,939,038 - ygdE b2806 0.79 -1.2 2,939,298 - ygdD b2807 0.10 +0.2 2,939,347 + ygdD b2807 0.22 -1.1 2,939,405 + ygdD b2807 0.37 +0.4 2,939,442 - ygdD b2807 0.46 +0.0 2,939,465 - ygdD b2807 0.52 +0.7 2,939,470 + ygdD b2807 0.54 -3.2 2,939,470 + ygdD b2807 0.54 -0.7 2,939,545 + ygdD b2807 0.72 +1.4 2,939,553 - ygdD b2807 0.74 +0.0 2,939,648 + -2.5 2,939,648 + -0.5 2,939,655 + -0.9 2,939,663 - +0.3 2,939,663 - -1.7 2,939,778 - gcvA b2808 0.12 -0.1 2,939,778 - gcvA b2808 0.12 -1.1 2,939,804 + gcvA b2808 0.14 -0.1 2,939,912 - gcvA b2808 0.26 -2.0 2,939,914 - gcvA b2808 0.26 -1.5 2,939,982 - gcvA b2808 0.34 +1.4 2,939,982 - gcvA b2808 0.34 -2.0 2,939,985 - gcvA b2808 0.34 -2.6 2,939,987 - gcvA b2808 0.34 -1.1 2,940,001 + gcvA b2808 0.36 -1.7 2,940,068 + gcvA b2808 0.43 -2.3 2,940,171 - gcvA b2808 0.54 +0.9 2,940,171 - gcvA b2808 0.54 -0.7 2,940,289 + gcvA b2808 0.67 -1.6 2,940,331 + gcvA b2808 0.72 -1.5 2,940,399 - gcvA b2808 0.79 -1.5 2,940,417 - gcvA b2808 0.81 +0.4 2,940,477 - gcvA b2808 0.88 -2.0 2,940,522 + -2.3 2,940,587 - +0.7
Or see this region's nucleotide sequence