Experiment: Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uidR and hdhA are separated by 220 nucleotides hdhA and malI are separated by 111 nucleotides
b1618: uidR - DNA-binding transcriptional repressor (NCBI), at 1,694,486 to 1,695,076
uidR
b1619: hdhA - 7-alpha-hydroxysteroid dehydrogenase (NCBI), at 1,695,297 to 1,696,064
hdhA
b1620: malI - DNA-binding transcriptional repressor (NCBI), at 1,696,176 to 1,697,204
malI
Position (kb)
1695
1696
1697 Strain fitness (log2 ratio)
-1
0
1
2 at 1694.629 kb on + strand at 1694.629 kb on + strand, within uidR at 1694.746 kb on - strand, within uidR at 1694.873 kb on + strand, within uidR at 1694.873 kb on + strand, within uidR at 1694.938 kb on + strand, within uidR at 1695.083 kb on + strand at 1695.089 kb on - strand at 1695.234 kb on - strand at 1695.316 kb on - strand at 1695.368 kb on + strand at 1695.368 kb on + strand at 1695.393 kb on - strand, within hdhA at 1695.425 kb on + strand, within hdhA at 1695.432 kb on - strand, within hdhA at 1695.432 kb on - strand, within hdhA at 1695.558 kb on - strand, within hdhA at 1695.574 kb on + strand, within hdhA at 1695.574 kb on + strand, within hdhA at 1695.574 kb on + strand, within hdhA at 1695.582 kb on - strand, within hdhA at 1695.582 kb on - strand, within hdhA at 1695.592 kb on - strand, within hdhA at 1695.592 kb on - strand, within hdhA at 1695.690 kb on - strand, within hdhA at 1695.690 kb on - strand, within hdhA at 1695.724 kb on - strand, within hdhA at 1695.758 kb on - strand, within hdhA at 1695.771 kb on - strand, within hdhA at 1695.771 kb on - strand, within hdhA at 1695.779 kb on - strand, within hdhA at 1695.779 kb on - strand, within hdhA at 1695.841 kb on - strand, within hdhA at 1695.841 kb on - strand, within hdhA at 1695.901 kb on - strand, within hdhA at 1695.914 kb on - strand, within hdhA at 1695.925 kb on - strand, within hdhA at 1695.959 kb on + strand, within hdhA at 1695.959 kb on + strand, within hdhA at 1696.001 kb on - strand at 1696.125 kb on - strand at 1696.125 kb on - strand at 1696.151 kb on - strand at 1696.154 kb on + strand at 1696.154 kb on + strand at 1696.154 kb on + strand at 1696.161 kb on - strand at 1696.161 kb on - strand at 1696.163 kb on - strand at 1696.163 kb on - strand at 1696.176 kb on + strand at 1696.176 kb on + strand at 1696.181 kb on - strand at 1696.181 kb on - strand at 1696.314 kb on + strand, within malI at 1696.330 kb on - strand, within malI at 1696.599 kb on - strand, within malI at 1696.617 kb on + strand, within malI at 1696.643 kb on + strand, within malI
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml remove 1,694,629 + +1.4 1,694,629 + uidR b1618 0.24 +1.4 1,694,746 - uidR b1618 0.44 -0.7 1,694,873 + uidR b1618 0.65 +2.2 1,694,873 + uidR b1618 0.65 +0.4 1,694,938 + uidR b1618 0.76 +0.8 1,695,083 + -0.2 1,695,089 - +1.4 1,695,234 - -0.0 1,695,316 - +0.6 1,695,368 + +1.2 1,695,368 + +0.6 1,695,393 - hdhA b1619 0.12 +0.7 1,695,425 + hdhA b1619 0.17 -1.0 1,695,432 - hdhA b1619 0.18 +0.4 1,695,432 - hdhA b1619 0.18 -0.1 1,695,558 - hdhA b1619 0.34 +0.4 1,695,574 + hdhA b1619 0.36 +0.8 1,695,574 + hdhA b1619 0.36 +1.0 1,695,574 + hdhA b1619 0.36 +0.4 1,695,582 - hdhA b1619 0.37 -0.6 1,695,582 - hdhA b1619 0.37 +1.4 1,695,592 - hdhA b1619 0.38 +1.0 1,695,592 - hdhA b1619 0.38 +0.8 1,695,690 - hdhA b1619 0.51 -0.6 1,695,690 - hdhA b1619 0.51 +0.9 1,695,724 - hdhA b1619 0.56 +1.4 1,695,758 - hdhA b1619 0.60 +0.5 1,695,771 - hdhA b1619 0.62 +0.6 1,695,771 - hdhA b1619 0.62 +1.2 1,695,779 - hdhA b1619 0.63 +1.3 1,695,779 - hdhA b1619 0.63 +0.2 1,695,841 - hdhA b1619 0.71 +0.9 1,695,841 - hdhA b1619 0.71 +0.3 1,695,901 - hdhA b1619 0.79 +1.2 1,695,914 - hdhA b1619 0.80 +0.2 1,695,925 - hdhA b1619 0.82 +0.4 1,695,959 + hdhA b1619 0.86 -1.6 1,695,959 + hdhA b1619 0.86 +1.1 1,696,001 - +1.0 1,696,125 - -0.1 1,696,125 - +1.0 1,696,151 - -0.5 1,696,154 + -0.1 1,696,154 + -0.6 1,696,154 + -0.9 1,696,161 - +1.0 1,696,161 - -0.3 1,696,163 - +0.6 1,696,163 - -0.8 1,696,176 + -0.0 1,696,176 + +0.6 1,696,181 - +0.3 1,696,181 - +0.9 1,696,314 + malI b1620 0.13 +0.5 1,696,330 - malI b1620 0.15 +0.4 1,696,599 - malI b1620 0.41 +0.8 1,696,617 + malI b1620 0.43 +1.2 1,696,643 + malI b1620 0.45 +0.8
Or see this region's nucleotide sequence