Experiment: Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prpD and prpE are separated by 39 nucleotides prpE and codB are separated by 329 nucleotides
b0334: prpD - 2-methylcitrate dehydratase (NCBI), at 350,439 to 351,890
prpD
b0335: prpE - predicted propionyl-CoA synthetase with ATPase domain (NCBI), at 351,930 to 353,816
prpE
b0336: codB - cytosine transporter (NCBI), at 354,146 to 355,405
codB
Position (kb) 351
352
353
354 Strain fitness (log2 ratio) -2
-1
0
1
2 at 351.073 kb on + strand, within prpD at 351.080 kb on + strand, within prpD at 351.080 kb on + strand, within prpD at 351.236 kb on + strand, within prpD at 351.419 kb on - strand, within prpD at 351.481 kb on - strand, within prpD at 351.565 kb on + strand, within prpD at 351.573 kb on - strand, within prpD at 351.691 kb on + strand, within prpD at 351.691 kb on + strand, within prpD at 351.702 kb on + strand, within prpD at 351.707 kb on - strand, within prpD at 351.918 kb on - strand at 351.918 kb on - strand at 351.977 kb on + strand at 351.977 kb on + strand at 351.977 kb on + strand at 352.027 kb on - strand at 352.027 kb on - strand at 352.185 kb on - strand, within prpE at 352.242 kb on + strand, within prpE at 352.242 kb on + strand, within prpE at 352.626 kb on + strand, within prpE at 352.648 kb on + strand, within prpE at 352.753 kb on + strand, within prpE at 352.783 kb on + strand, within prpE at 352.813 kb on - strand, within prpE at 352.813 kb on - strand, within prpE at 352.871 kb on + strand, within prpE at 352.901 kb on + strand, within prpE at 352.901 kb on + strand, within prpE at 353.041 kb on + strand, within prpE at 353.051 kb on + strand, within prpE at 353.151 kb on + strand, within prpE at 353.151 kb on + strand, within prpE at 353.197 kb on + strand, within prpE at 353.241 kb on - strand, within prpE at 353.346 kb on + strand, within prpE at 353.346 kb on + strand, within prpE at 353.363 kb on - strand, within prpE at 353.440 kb on + strand, within prpE at 353.465 kb on + strand, within prpE at 353.589 kb on + strand, within prpE at 353.589 kb on + strand, within prpE at 353.746 kb on + strand at 353.957 kb on + strand at 353.966 kb on - strand at 354.098 kb on - strand at 354.174 kb on + strand at 354.254 kb on + strand at 354.311 kb on + strand, within codB at 354.384 kb on + strand, within codB at 354.386 kb on - strand, within codB at 354.423 kb on - strand, within codB at 354.431 kb on - strand, within codB at 354.480 kb on + strand, within codB at 354.773 kb on - strand, within codB at 354.773 kb on - strand, within codB
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml remove 351,073 + prpD b0334 0.44 +1.4 351,080 + prpD b0334 0.44 +0.4 351,080 + prpD b0334 0.44 -0.5 351,236 + prpD b0334 0.55 +0.1 351,419 - prpD b0334 0.67 -0.5 351,481 - prpD b0334 0.72 -0.5 351,565 + prpD b0334 0.78 +0.4 351,573 - prpD b0334 0.78 -1.4 351,691 + prpD b0334 0.86 +2.4 351,691 + prpD b0334 0.86 +2.4 351,702 + prpD b0334 0.87 +0.2 351,707 - prpD b0334 0.87 -1.5 351,918 - +0.2 351,918 - -0.4 351,977 + +0.4 351,977 + +0.6 351,977 + +0.6 352,027 - -2.2 352,027 - +1.4 352,185 - prpE b0335 0.14 +0.7 352,242 + prpE b0335 0.17 +0.0 352,242 + prpE b0335 0.17 -0.7 352,626 + prpE b0335 0.37 +0.3 352,648 + prpE b0335 0.38 +0.5 352,753 + prpE b0335 0.44 +0.8 352,783 + prpE b0335 0.45 +0.6 352,813 - prpE b0335 0.47 +0.7 352,813 - prpE b0335 0.47 -0.0 352,871 + prpE b0335 0.50 +0.4 352,901 + prpE b0335 0.51 +1.8 352,901 + prpE b0335 0.51 +0.1 353,041 + prpE b0335 0.59 +0.5 353,051 + prpE b0335 0.59 -0.2 353,151 + prpE b0335 0.65 -0.4 353,151 + prpE b0335 0.65 -1.2 353,197 + prpE b0335 0.67 +0.8 353,241 - prpE b0335 0.69 -0.3 353,346 + prpE b0335 0.75 -0.0 353,346 + prpE b0335 0.75 +0.6 353,363 - prpE b0335 0.76 -0.5 353,440 + prpE b0335 0.80 +0.6 353,465 + prpE b0335 0.81 +0.6 353,589 + prpE b0335 0.88 -0.7 353,589 + prpE b0335 0.88 -1.0 353,746 + +1.0 353,957 + +0.3 353,966 - +0.3 354,098 - -0.0 354,174 + -0.5 354,254 + -1.5 354,311 + codB b0336 0.13 -1.3 354,384 + codB b0336 0.19 +1.2 354,386 - codB b0336 0.19 +1.2 354,423 - codB b0336 0.22 -0.5 354,431 - codB b0336 0.23 -0.7 354,480 + codB b0336 0.27 +2.5 354,773 - codB b0336 0.50 +1.3 354,773 - codB b0336 0.50 -1.4
Or see this region's nucleotide sequence