Experiment: Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hpt and can are separated by 40 nucleotides can and yadG are separated by 108 nucleotides yadG and yadH overlap by 4 nucleotides yadH and yadI are separated by 104 nucleotides
b0125: hpt - hypoxanthine phosphoribosyltransferase (VIMSS), at 141,431 to 141,967
hpt
b0126: can - carbonic anhydrase (NCBI), at 142,008 to 142,670
can
b0127: yadG - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 142,779 to 143,705
yadG
b0128: yadH - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 143,702 to 144,472
yadH
b0129: yadI - predicted PTS Enzyme IIA (NCBI), at 144,577 to 145,017
yadI
Position (kb) 142
143
144 Strain fitness (log2 ratio) -1
0
1
2
3 at 141.899 kb on + strand, within hpt at 141.906 kb on + strand, within hpt at 141.910 kb on + strand, within hpt at 141.910 kb on + strand, within hpt at 141.967 kb on + strand at 142.000 kb on - strand at 142.000 kb on - strand at 142.184 kb on - strand, within can at 142.836 kb on - strand at 142.836 kb on - strand at 142.925 kb on - strand, within yadG at 143.022 kb on - strand, within yadG at 143.051 kb on + strand, within yadG at 143.490 kb on - strand, within yadG at 143.532 kb on - strand, within yadG at 143.532 kb on - strand, within yadG at 143.532 kb on - strand, within yadG at 143.554 kb on + strand, within yadG at 143.554 kb on + strand, within yadG at 143.585 kb on - strand, within yadG at 143.590 kb on + strand, within yadG at 143.590 kb on + strand, within yadG at 143.607 kb on - strand, within yadG at 143.620 kb on - strand at 143.621 kb on + strand at 143.744 kb on + strand at 143.757 kb on + strand at 143.782 kb on - strand, within yadH at 143.790 kb on + strand, within yadH at 143.815 kb on + strand, within yadH at 143.824 kb on + strand, within yadH at 143.845 kb on + strand, within yadH at 143.879 kb on + strand, within yadH at 143.899 kb on + strand, within yadH at 143.912 kb on + strand, within yadH at 143.986 kb on + strand, within yadH at 144.160 kb on - strand, within yadH at 144.218 kb on + strand, within yadH at 144.256 kb on - strand, within yadH at 144.302 kb on + strand, within yadH at 144.304 kb on + strand, within yadH at 144.304 kb on + strand, within yadH at 144.331 kb on + strand, within yadH at 144.333 kb on + strand, within yadH at 144.386 kb on + strand, within yadH at 144.386 kb on + strand, within yadH at 144.403 kb on + strand at 144.403 kb on + strand at 144.440 kb on - strand at 144.539 kb on + strand at 144.539 kb on + strand at 144.539 kb on + strand at 144.539 kb on + strand at 144.630 kb on - strand, within yadI at 144.630 kb on - strand, within yadI at 144.663 kb on + strand, within yadI at 144.663 kb on + strand, within yadI
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.001 mg/ml remove 141,899 + hpt b0125 0.87 +1.1 141,906 + hpt b0125 0.88 +1.1 141,910 + hpt b0125 0.89 +0.5 141,910 + hpt b0125 0.89 +3.2 141,967 + +0.2 142,000 - +0.1 142,000 - +0.5 142,184 - can b0126 0.27 +0.9 142,836 - +0.2 142,836 - -0.6 142,925 - yadG b0127 0.16 +0.4 143,022 - yadG b0127 0.26 +0.9 143,051 + yadG b0127 0.29 +0.6 143,490 - yadG b0127 0.77 -0.3 143,532 - yadG b0127 0.81 +1.1 143,532 - yadG b0127 0.81 +0.1 143,532 - yadG b0127 0.81 +1.6 143,554 + yadG b0127 0.84 +0.9 143,554 + yadG b0127 0.84 +0.2 143,585 - yadG b0127 0.87 +1.6 143,590 + yadG b0127 0.87 +1.2 143,590 + yadG b0127 0.87 +1.2 143,607 - yadG b0127 0.89 +0.2 143,620 - -0.4 143,621 + -0.4 143,744 + -0.4 143,757 + +0.9 143,782 - yadH b0128 0.10 +2.9 143,790 + yadH b0128 0.11 +0.5 143,815 + yadH b0128 0.15 -1.2 143,824 + yadH b0128 0.16 +1.6 143,845 + yadH b0128 0.19 -1.0 143,879 + yadH b0128 0.23 +0.3 143,899 + yadH b0128 0.26 -0.5 143,912 + yadH b0128 0.27 +0.2 143,986 + yadH b0128 0.37 +0.0 144,160 - yadH b0128 0.59 +0.4 144,218 + yadH b0128 0.67 +0.6 144,256 - yadH b0128 0.72 +0.8 144,302 + yadH b0128 0.78 +0.3 144,304 + yadH b0128 0.78 -0.1 144,304 + yadH b0128 0.78 +0.1 144,331 + yadH b0128 0.82 +0.7 144,333 + yadH b0128 0.82 -0.9 144,386 + yadH b0128 0.89 +1.9 144,386 + yadH b0128 0.89 +0.4 144,403 + -0.4 144,403 + +2.1 144,440 - +0.6 144,539 + -0.1 144,539 + +1.3 144,539 + +1.2 144,539 + +0.5 144,630 - yadI b0129 0.12 +0.0 144,630 - yadI b0129 0.12 -0.1 144,663 + yadI b0129 0.20 +0.8 144,663 + yadI b0129 0.20 -0.3
Or see this region's nucleotide sequence