Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_29 and PfGW456L13_30 are separated by 223 nucleotides PfGW456L13_30 and PfGW456L13_31 are separated by 2 nucleotides PfGW456L13_31 and PfGW456L13_32 are separated by 58 nucleotides
PfGW456L13_29 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 34,910 to 36,187
_29
PfGW456L13_30 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 36,411 to 37,763
_30
PfGW456L13_31 - Acyl dehydratase, at 37,766 to 38,620
_31
PfGW456L13_32 - hypothetical protein, at 38,679 to 39,035
_32
Position (kb)
36
37
38 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 35.451 kb on + strand, within PfGW456L13_29 at 35.452 kb on - strand, within PfGW456L13_29 at 35.452 kb on - strand, within PfGW456L13_29 at 35.452 kb on - strand, within PfGW456L13_29 at 35.934 kb on + strand, within PfGW456L13_29 at 35.934 kb on + strand, within PfGW456L13_29 at 35.934 kb on + strand, within PfGW456L13_29 at 35.934 kb on + strand, within PfGW456L13_29 at 35.934 kb on + strand, within PfGW456L13_29 at 35.935 kb on - strand, within PfGW456L13_29 at 35.935 kb on - strand, within PfGW456L13_29 at 35.970 kb on - strand, within PfGW456L13_29 at 35.970 kb on - strand, within PfGW456L13_29 at 36.057 kb on + strand, within PfGW456L13_29 at 36.057 kb on + strand, within PfGW456L13_29 at 36.058 kb on - strand, within PfGW456L13_29 at 36.058 kb on - strand, within PfGW456L13_29 at 36.058 kb on - strand, within PfGW456L13_29 at 36.118 kb on - strand at 36.118 kb on - strand at 36.286 kb on + strand at 36.286 kb on + strand at 36.287 kb on - strand at 36.287 kb on - strand at 36.287 kb on - strand at 36.315 kb on + strand at 36.315 kb on + strand at 36.315 kb on + strand at 36.315 kb on + strand at 36.315 kb on + strand at 36.315 kb on + strand at 36.316 kb on - strand at 36.316 kb on - strand at 36.316 kb on - strand at 36.316 kb on - strand at 36.332 kb on + strand at 36.332 kb on + strand at 36.332 kb on + strand at 36.332 kb on + strand at 36.333 kb on - strand at 36.339 kb on + strand at 36.339 kb on + strand at 36.340 kb on - strand at 36.340 kb on - strand at 36.424 kb on - strand at 36.424 kb on - strand at 36.424 kb on - strand at 36.424 kb on - strand at 36.426 kb on - strand at 36.529 kb on + strand at 36.529 kb on + strand at 36.529 kb on + strand at 36.529 kb on + strand at 36.530 kb on - strand at 36.530 kb on - strand at 36.530 kb on - strand at 36.530 kb on - strand at 36.609 kb on + strand, within PfGW456L13_30 at 36.609 kb on + strand, within PfGW456L13_30 at 36.609 kb on + strand, within PfGW456L13_30 at 36.609 kb on + strand, within PfGW456L13_30 at 36.609 kb on + strand, within PfGW456L13_30 at 36.610 kb on - strand, within PfGW456L13_30 at 36.610 kb on - strand, within PfGW456L13_30 at 36.610 kb on - strand, within PfGW456L13_30 at 36.610 kb on - strand, within PfGW456L13_30 at 36.615 kb on + strand, within PfGW456L13_30 at 36.615 kb on + strand, within PfGW456L13_30 at 36.615 kb on + strand, within PfGW456L13_30 at 36.615 kb on + strand, within PfGW456L13_30 at 36.615 kb on + strand, within PfGW456L13_30 at 36.615 kb on + strand, within PfGW456L13_30 at 36.616 kb on - strand, within PfGW456L13_30 at 36.616 kb on - strand, within PfGW456L13_30 at 36.877 kb on + strand, within PfGW456L13_30 at 36.878 kb on - strand, within PfGW456L13_30 at 36.906 kb on + strand, within PfGW456L13_30 at 36.906 kb on + strand, within PfGW456L13_30 at 36.906 kb on + strand, within PfGW456L13_30 at 36.906 kb on + strand, within PfGW456L13_30 at 36.906 kb on + strand, within PfGW456L13_30 at 36.906 kb on + strand, within PfGW456L13_30 at 36.906 kb on + strand, within PfGW456L13_30 at 36.906 kb on + strand, within PfGW456L13_30 at 36.907 kb on - strand, within PfGW456L13_30 at 36.907 kb on - strand, within PfGW456L13_30 at 36.907 kb on - strand, within PfGW456L13_30 at 36.907 kb on - strand, within PfGW456L13_30 at 36.907 kb on - strand, within PfGW456L13_30 at 36.907 kb on - strand, within PfGW456L13_30 at 37.330 kb on - strand, within PfGW456L13_30 at 37.372 kb on + strand, within PfGW456L13_30 at 37.372 kb on + strand, within PfGW456L13_30 at 37.372 kb on + strand, within PfGW456L13_30 at 37.373 kb on - strand, within PfGW456L13_30 at 37.373 kb on - strand, within PfGW456L13_30 at 37.476 kb on + strand, within PfGW456L13_30 at 37.736 kb on - strand at 37.761 kb on + strand at 37.761 kb on + strand at 37.817 kb on + strand at 37.818 kb on - strand at 37.818 kb on - strand at 37.818 kb on - strand at 37.964 kb on + strand, within PfGW456L13_31 at 37.964 kb on + strand, within PfGW456L13_31 at 37.965 kb on - strand, within PfGW456L13_31 at 38.068 kb on - strand, within PfGW456L13_31 at 38.068 kb on - strand, within PfGW456L13_31 at 38.345 kb on + strand, within PfGW456L13_31 at 38.345 kb on + strand, within PfGW456L13_31 at 38.345 kb on + strand, within PfGW456L13_31 at 38.346 kb on - strand, within PfGW456L13_31 at 38.590 kb on + strand at 38.590 kb on + strand at 38.590 kb on + strand at 38.590 kb on + strand at 38.590 kb on + strand at 38.591 kb on - strand at 38.591 kb on - strand at 38.591 kb on - strand at 38.591 kb on - strand at 38.591 kb on - strand at 38.591 kb on - strand at 38.631 kb on - strand at 38.699 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 35,451 + PfGW456L13_29 0.42 -0.8 35,452 - PfGW456L13_29 0.42 +2.4 35,452 - PfGW456L13_29 0.42 -0.7 35,452 - PfGW456L13_29 0.42 +1.1 35,934 + PfGW456L13_29 0.80 -2.6 35,934 + PfGW456L13_29 0.80 -0.2 35,934 + PfGW456L13_29 0.80 -0.8 35,934 + PfGW456L13_29 0.80 -1.0 35,934 + PfGW456L13_29 0.80 +0.4 35,935 - PfGW456L13_29 0.80 +1.0 35,935 - PfGW456L13_29 0.80 -2.6 35,970 - PfGW456L13_29 0.83 +0.5 35,970 - PfGW456L13_29 0.83 +0.6 36,057 + PfGW456L13_29 0.90 +1.3 36,057 + PfGW456L13_29 0.90 -0.2 36,058 - PfGW456L13_29 0.90 -1.0 36,058 - PfGW456L13_29 0.90 +2.0 36,058 - PfGW456L13_29 0.90 +2.9 36,118 - +1.8 36,118 - +0.6 36,286 + +1.6 36,286 + +0.4 36,287 - -2.4 36,287 - +1.1 36,287 - -1.7 36,315 + -2.4 36,315 + -2.8 36,315 + +0.3 36,315 + +0.4 36,315 + -3.1 36,315 + +1.4 36,316 - -0.8 36,316 - -2.1 36,316 - -2.8 36,316 - -0.8 36,332 + -3.1 36,332 + +0.7 36,332 + -2.6 36,332 + -2.6 36,333 - +0.5 36,339 + -2.8 36,339 + +0.7 36,340 - +1.4 36,340 - +1.0 36,424 - +0.8 36,424 - +2.0 36,424 - +0.3 36,424 - +2.5 36,426 - -0.1 36,529 + -0.2 36,529 + -2.5 36,529 + +2.7 36,529 + +1.6 36,530 - -2.0 36,530 - +1.8 36,530 - -0.7 36,530 - -3.7 36,609 + PfGW456L13_30 0.15 +1.1 36,609 + PfGW456L13_30 0.15 +0.2 36,609 + PfGW456L13_30 0.15 +0.9 36,609 + PfGW456L13_30 0.15 -0.3 36,609 + PfGW456L13_30 0.15 -1.0 36,610 - PfGW456L13_30 0.15 +1.0 36,610 - PfGW456L13_30 0.15 -2.5 36,610 - PfGW456L13_30 0.15 +1.2 36,610 - PfGW456L13_30 0.15 +0.8 36,615 + PfGW456L13_30 0.15 -2.6 36,615 + PfGW456L13_30 0.15 -1.8 36,615 + PfGW456L13_30 0.15 +1.0 36,615 + PfGW456L13_30 0.15 +2.4 36,615 + PfGW456L13_30 0.15 -1.2 36,615 + PfGW456L13_30 0.15 -1.3 36,616 - PfGW456L13_30 0.15 -2.5 36,616 - PfGW456L13_30 0.15 +2.2 36,877 + PfGW456L13_30 0.34 +3.5 36,878 - PfGW456L13_30 0.35 -1.4 36,906 + PfGW456L13_30 0.37 -1.0 36,906 + PfGW456L13_30 0.37 -1.4 36,906 + PfGW456L13_30 0.37 -1.6 36,906 + PfGW456L13_30 0.37 -3.1 36,906 + PfGW456L13_30 0.37 -1.4 36,906 + PfGW456L13_30 0.37 +1.3 36,906 + PfGW456L13_30 0.37 -3.4 36,906 + PfGW456L13_30 0.37 +1.0 36,907 - PfGW456L13_30 0.37 +0.7 36,907 - PfGW456L13_30 0.37 -2.3 36,907 - PfGW456L13_30 0.37 -1.4 36,907 - PfGW456L13_30 0.37 +1.2 36,907 - PfGW456L13_30 0.37 -1.7 36,907 - PfGW456L13_30 0.37 +1.6 37,330 - PfGW456L13_30 0.68 -0.5 37,372 + PfGW456L13_30 0.71 -0.2 37,372 + PfGW456L13_30 0.71 -0.4 37,372 + PfGW456L13_30 0.71 +0.8 37,373 - PfGW456L13_30 0.71 +1.9 37,373 - PfGW456L13_30 0.71 +1.8 37,476 + PfGW456L13_30 0.79 -1.2 37,736 - +0.4 37,761 + -2.9 37,761 + -2.5 37,817 + +2.0 37,818 - +0.4 37,818 - -1.0 37,818 - -1.7 37,964 + PfGW456L13_31 0.23 -2.5 37,964 + PfGW456L13_31 0.23 +1.2 37,965 - PfGW456L13_31 0.23 +2.8 38,068 - PfGW456L13_31 0.35 +1.4 38,068 - PfGW456L13_31 0.35 +1.2 38,345 + PfGW456L13_31 0.68 -2.4 38,345 + PfGW456L13_31 0.68 -3.5 38,345 + PfGW456L13_31 0.68 -1.2 38,346 - PfGW456L13_31 0.68 -0.4 38,590 + +2.1 38,590 + +1.5 38,590 + +0.6 38,590 + +0.1 38,590 + -3.0 38,591 - -1.9 38,591 - -0.2 38,591 - +1.8 38,591 - +0.2 38,591 - -1.0 38,591 - +1.5 38,631 - +1.0 38,699 + +0.1
Or see this region's nucleotide sequence