Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_259 and PfGW456L13_260 overlap by 4 nucleotides PfGW456L13_260 and PfGW456L13_261 are separated by 16 nucleotides PfGW456L13_261 and PfGW456L13_262 are separated by 19 nucleotides PfGW456L13_262 and PfGW456L13_263 are separated by 42 nucleotides PfGW456L13_263 and PfGW456L13_264 are separated by 3 nucleotides
PfGW456L13_259 - General secretion pathway protein K, at 303,401 to 304,252
_259
PfGW456L13_260 - General secretion pathway protein J, at 304,249 to 304,767
_260
PfGW456L13_261 - General secretion pathway protein I, at 304,784 to 305,140
_261
PfGW456L13_262 - hypothetical protein, at 305,160 to 305,588
_262
PfGW456L13_263 - General secretion pathway protein G, at 305,631 to 306,086
_263
PfGW456L13_264 - General secretion pathway protein F, at 306,090 to 307,295
_264
Position (kb) 304
305
306 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 304.055 kb on + strand, within PfGW456L13_259 at 304.214 kb on + strand at 304.214 kb on + strand at 304.214 kb on + strand at 304.215 kb on - strand at 304.215 kb on - strand at 304.215 kb on - strand at 304.215 kb on - strand at 304.215 kb on - strand at 304.215 kb on - strand at 304.215 kb on - strand at 304.215 kb on - strand at 304.342 kb on - strand, within PfGW456L13_260 at 304.342 kb on - strand, within PfGW456L13_260 at 304.497 kb on - strand, within PfGW456L13_260 at 304.504 kb on + strand, within PfGW456L13_260 at 304.505 kb on - strand, within PfGW456L13_260 at 304.786 kb on - strand at 304.802 kb on + strand at 304.821 kb on + strand, within PfGW456L13_261 at 304.871 kb on + strand, within PfGW456L13_261 at 304.872 kb on - strand, within PfGW456L13_261 at 304.979 kb on + strand, within PfGW456L13_261 at 304.980 kb on - strand, within PfGW456L13_261 at 305.055 kb on + strand, within PfGW456L13_261 at 305.056 kb on - strand, within PfGW456L13_261 at 305.314 kb on + strand, within PfGW456L13_262 at 305.335 kb on - strand, within PfGW456L13_262 at 305.335 kb on - strand, within PfGW456L13_262 at 305.335 kb on - strand, within PfGW456L13_262 at 305.437 kb on + strand, within PfGW456L13_262 at 305.438 kb on - strand, within PfGW456L13_262 at 305.603 kb on + strand at 305.603 kb on + strand at 305.604 kb on - strand at 305.604 kb on - strand at 305.604 kb on - strand at 305.680 kb on - strand, within PfGW456L13_263 at 305.701 kb on + strand, within PfGW456L13_263 at 305.710 kb on + strand, within PfGW456L13_263 at 305.710 kb on + strand, within PfGW456L13_263 at 305.711 kb on - strand, within PfGW456L13_263 at 305.711 kb on - strand, within PfGW456L13_263 at 305.711 kb on - strand, within PfGW456L13_263 at 305.711 kb on - strand at 305.711 kb on - strand, within PfGW456L13_263 at 305.711 kb on - strand, within PfGW456L13_263 at 305.721 kb on + strand, within PfGW456L13_263 at 305.722 kb on - strand, within PfGW456L13_263 at 305.747 kb on - strand, within PfGW456L13_263 at 305.752 kb on + strand, within PfGW456L13_263 at 305.752 kb on + strand, within PfGW456L13_263 at 305.752 kb on + strand, within PfGW456L13_263 at 305.760 kb on - strand, within PfGW456L13_263 at 305.760 kb on - strand at 305.896 kb on + strand, within PfGW456L13_263 at 305.897 kb on - strand, within PfGW456L13_263 at 306.016 kb on - strand, within PfGW456L13_263 at 306.016 kb on - strand, within PfGW456L13_263 at 306.042 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 304,055 + PfGW456L13_259 0.77 -0.6 304,214 + +0.8 304,214 + +1.0 304,214 + +0.8 304,215 - +0.8 304,215 - -0.3 304,215 - +1.3 304,215 - -2.2 304,215 - -3.3 304,215 - +0.7 304,215 - -2.4 304,215 - +1.2 304,342 - PfGW456L13_260 0.18 -3.0 304,342 - PfGW456L13_260 0.18 +0.6 304,497 - PfGW456L13_260 0.48 +1.2 304,504 + PfGW456L13_260 0.49 +3.4 304,505 - PfGW456L13_260 0.49 -1.2 304,786 - -2.7 304,802 + -1.3 304,821 + PfGW456L13_261 0.10 -2.0 304,871 + PfGW456L13_261 0.24 +1.6 304,872 - PfGW456L13_261 0.25 +1.4 304,979 + PfGW456L13_261 0.55 +2.3 304,980 - PfGW456L13_261 0.55 +1.6 305,055 + PfGW456L13_261 0.76 -2.3 305,056 - PfGW456L13_261 0.76 +0.6 305,314 + PfGW456L13_262 0.36 +0.9 305,335 - PfGW456L13_262 0.41 -2.3 305,335 - PfGW456L13_262 0.41 +0.4 305,335 - PfGW456L13_262 0.41 -4.2 305,437 + PfGW456L13_262 0.65 +2.0 305,438 - PfGW456L13_262 0.65 +0.7 305,603 + -4.0 305,603 + +0.4 305,604 - +1.1 305,604 - -3.8 305,604 - +1.5 305,680 - PfGW456L13_263 0.11 -2.4 305,701 + PfGW456L13_263 0.15 -1.4 305,710 + PfGW456L13_263 0.17 -3.1 305,710 + PfGW456L13_263 0.17 -1.0 305,711 - PfGW456L13_263 0.18 -0.9 305,711 - PfGW456L13_263 0.18 -1.8 305,711 - PfGW456L13_263 0.18 +1.2 305,711 - -0.2 305,711 - PfGW456L13_263 0.18 -0.0 305,711 - PfGW456L13_263 0.18 -3.4 305,721 + PfGW456L13_263 0.20 -2.4 305,722 - PfGW456L13_263 0.20 -2.9 305,747 - PfGW456L13_263 0.25 +0.5 305,752 + PfGW456L13_263 0.27 +1.6 305,752 + PfGW456L13_263 0.27 -1.9 305,752 + PfGW456L13_263 0.27 -2.9 305,760 - PfGW456L13_263 0.28 -2.7 305,760 - -0.2 305,896 + PfGW456L13_263 0.58 -0.2 305,897 - PfGW456L13_263 0.58 +1.1 306,016 - PfGW456L13_263 0.84 +2.8 306,016 - PfGW456L13_263 0.84 +1.0 306,042 - +0.1
Or see this region's nucleotide sequence