Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_158

Experiment: a-Ketoglutaric (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_157 and PfGW456L13_158 are separated by 15 nucleotidesPfGW456L13_158 and PfGW456L13_159 overlap by 13 nucleotides PfGW456L13_157 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 181,841 to 183,256 _157 PfGW456L13_158 - TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 183,272 to 183,742 _158 PfGW456L13_159 - NAD(P)HX epimerase / NAD(P)HX dehydratase, at 183,730 to 185,229 _159 Position (kb) 183 184Strain fitness (log2 ratio) -3 -2 -1 0 1at 183.257 kb on - strandat 183.257 kb on - strandat 183.274 kb on - strandat 183.274 kb on - strandat 183.274 kb on - strandat 183.274 kb on - strandat 183.274 kb on - strandat 184.030 kb on - strand, within PfGW456L13_159at 184.030 kb on - strand, within PfGW456L13_159at 184.545 kb on + strand, within PfGW456L13_159at 184.546 kb on - strand, within PfGW456L13_159at 184.573 kb on - strand, within PfGW456L13_159

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Per-strain Table

Position Strand Gene LocusTag Fraction a-Ketoglutaric (C)
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183,257 - -2.3
183,257 - +1.4
183,274 - -1.1
183,274 - +0.7
183,274 - -2.9
183,274 - -0.9
183,274 - +1.2
184,030 - PfGW456L13_159 0.20 -3.2
184,030 - PfGW456L13_159 0.20 +0.7
184,545 + PfGW456L13_159 0.54 +0.6
184,546 - PfGW456L13_159 0.54 -1.2
184,573 - PfGW456L13_159 0.56 -1.0

Or see this region's nucleotide sequence