Experiment: D-Trehalose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3480 and Atu3481 overlap by 11 nucleotides Atu3481 and Atu3482 are separated by 140 nucleotides Atu3482 and Atu3483 overlap by 4 nucleotides Atu3483 and Atu3484 are separated by 34 nucleotides
Atu3480: Atu3480 - hydroxymethylglutaryl-CoA lyase, at 529,307 to 530,233
Atu3480
Atu3481: Atu3481 - enoyl-CoA hydratase, at 530,223 to 530,999
Atu3481
Atu3482: Atu3482 - pantoate--beta-alanine ligase, at 531,140 to 532,018
Atu3482
Atu3483: Atu3483 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 532,015 to 532,854
Atu3483
Atu3484: Atu3484 - hypothetical protein, at 532,889 to 534,181
Atu3484
Position (kb) 531
532
533 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 530.206 kb on + strand at 530.493 kb on + strand, within Atu3481 at 530.520 kb on + strand, within Atu3481 at 530.533 kb on + strand, within Atu3481 at 530.703 kb on - strand, within Atu3481 at 530.737 kb on + strand, within Atu3481 at 530.786 kb on + strand, within Atu3481 at 530.993 kb on + strand at 531.060 kb on - strand at 531.092 kb on + strand at 531.223 kb on + strand at 531.276 kb on - strand, within Atu3482 at 531.422 kb on - strand, within Atu3482 at 531.446 kb on + strand, within Atu3482 at 531.586 kb on + strand, within Atu3482 at 531.628 kb on - strand, within Atu3482 at 531.628 kb on - strand, within Atu3482 at 531.628 kb on - strand, within Atu3482 at 531.710 kb on - strand, within Atu3482 at 531.772 kb on + strand, within Atu3482 at 531.923 kb on - strand, within Atu3482 at 532.099 kb on - strand, within Atu3483 at 532.169 kb on + strand, within Atu3483 at 532.320 kb on + strand, within Atu3483 at 532.479 kb on - strand, within Atu3483 at 532.609 kb on - strand, within Atu3483 at 532.767 kb on + strand, within Atu3483
Per-strain Table
Position Strand Gene LocusTag Fraction D-Trehalose (C) remove 530,206 + -0.1 530,493 + Atu3481 0.35 +0.2 530,520 + Atu3481 0.38 -0.2 530,533 + Atu3481 0.40 -0.2 530,703 - Atu3481 0.62 -0.5 530,737 + Atu3481 0.66 +1.9 530,786 + Atu3481 0.72 +0.3 530,993 + -0.2 531,060 - +0.3 531,092 + -0.6 531,223 + -1.9 531,276 - Atu3482 0.15 -4.9 531,422 - Atu3482 0.32 -4.2 531,446 + Atu3482 0.35 -3.6 531,586 + Atu3482 0.51 -2.5 531,628 - Atu3482 0.56 -6.0 531,628 - Atu3482 0.56 -3.2 531,628 - Atu3482 0.56 -4.5 531,710 - Atu3482 0.65 -4.2 531,772 + Atu3482 0.72 -3.7 531,923 - Atu3482 0.89 -3.4 532,099 - Atu3483 0.10 -3.1 532,169 + Atu3483 0.18 -3.8 532,320 + Atu3483 0.36 -6.5 532,479 - Atu3483 0.55 -3.8 532,609 - Atu3483 0.71 -2.1 532,767 + Atu3483 0.90 -1.9
Or see this region's nucleotide sequence