Experiment: 30_an_nonmag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS16275 and AMB_RS16280 overlap by 4 nucleotides AMB_RS16280 and AMB_RS16285 are separated by 184 nucleotides AMB_RS16285 and AMB_RS16290 are separated by 3 nucleotides AMB_RS16290 and AMB_RS16295 are separated by 157 nucleotides AMB_RS16295 and AMB_RS16300 overlap by 4 nucleotides
AMB_RS16275: AMB_RS16275 - metallophosphoesterase, at 3,486,666 to 3,487,484
_RS16275
AMB_RS16280: AMB_RS16280 - 5-formyltetrahydrofolate cyclo-ligase, at 3,487,481 to 3,488,062
_RS16280
AMB_RS16285: AMB_RS16285 - cell division protein ZapA, at 3,488,247 to 3,488,573
_RS16285
AMB_RS16290: AMB_RS16290 - hypothetical protein, at 3,488,577 to 3,488,789
_RS16290
AMB_RS16295: AMB_RS16295 - DUF3124 domain-containing protein, at 3,488,947 to 3,489,450
_RS16295
AMB_RS16300: AMB_RS16300 - hypothetical protein, at 3,489,447 to 3,489,773
_RS16300
Position (kb) 3488
3489 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3487.471 kb on + strand at 3487.471 kb on + strand at 3487.471 kb on + strand at 3487.472 kb on - strand at 3487.472 kb on - strand at 3487.472 kb on - strand at 3487.521 kb on - strand at 3487.521 kb on - strand at 3487.521 kb on - strand at 3487.641 kb on + strand, within AMB_RS16280 at 3487.641 kb on + strand, within AMB_RS16280 at 3487.642 kb on - strand, within AMB_RS16280 at 3487.642 kb on - strand, within AMB_RS16280 at 3487.642 kb on - strand, within AMB_RS16280 at 3487.838 kb on + strand, within AMB_RS16280 at 3487.838 kb on + strand, within AMB_RS16280 at 3487.838 kb on + strand, within AMB_RS16280 at 3487.839 kb on - strand, within AMB_RS16280 at 3487.905 kb on + strand, within AMB_RS16280 at 3488.140 kb on - strand at 3488.142 kb on - strand at 3488.185 kb on + strand at 3488.908 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_3 remove 3,487,471 + +0.4 3,487,471 + -3.3 3,487,471 + -0.9 3,487,472 - -2.4 3,487,472 - -1.1 3,487,472 - -1.4 3,487,521 - +0.4 3,487,521 - -1.9 3,487,521 - -0.2 3,487,641 + AMB_RS16280 0.27 -0.1 3,487,641 + AMB_RS16280 0.27 -1.7 3,487,642 - AMB_RS16280 0.28 +0.4 3,487,642 - AMB_RS16280 0.28 +0.4 3,487,642 - AMB_RS16280 0.28 -0.9 3,487,838 + AMB_RS16280 0.61 -1.9 3,487,838 + AMB_RS16280 0.61 -2.1 3,487,838 + AMB_RS16280 0.61 +1.3 3,487,839 - AMB_RS16280 0.62 -0.5 3,487,905 + AMB_RS16280 0.73 -1.1 3,488,140 - -1.1 3,488,142 - -2.1 3,488,185 + -1.6 3,488,908 + -0.1
Or see this region's nucleotide sequence